Identification of circRNA/miRNA/mRNA regulatory network involving (+)-catechin ameliorates diabetic nephropathy mice
(+)-Catechin (CE) is mainly found in green and black tea and has many biological effects, such as anti-inflammatory, anti-cancer, anti-viral effects, protecting human organs, especially the kidney. This study aims to identify the circRNAs induced by CE in db/db mice and their roles in diabetic nephr...
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Tsinghua University Press
2022-05-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453021001518 |
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| author | Chao Chen Dina Zhu Shuai Zhang Wensheng Zhang |
| author_facet | Chao Chen Dina Zhu Shuai Zhang Wensheng Zhang |
| author_sort | Chao Chen |
| collection | DOAJ |
| description | (+)-Catechin (CE) is mainly found in green and black tea and has many biological effects, such as anti-inflammatory, anti-cancer, anti-viral effects, protecting human organs, especially the kidney. This study aims to identify the circRNAs induced by CE in db/db mice and their roles in diabetic nephropathy progression. After the db/db mice were treated with CE, RNA-seq was performed to identify the differentially expressed circRNA and mRNAs. The ceRNA regulatory network was constructed and analyzed using bioinformatics software and public databases (Cytoscape, ClueGO, MiRWalk, STRING, et al.). Our results revealed that 6 differentially expressed circRNAs are most associated with the cholinergic synapse, neurotrophin signaling pathway, and insulin signaling pathway. Among these, circRNA.5549 and circRNA.4712 might regulate Cd36, Cyp26b1, C8a, Cyp2j13, Grem2 genes through ceRNA regulatory mechanism in the presence of CE treatment. The expanded network of proteins interacting with these 5 genes shows that the TGF-β signaling pathway, signaling pathways regulating pluripotency of stem cell, fat digestion and absorption, and PPAR signaling pathway was highly enriched. Overall, circRNA.5549 and circRNA.4712 exhibit a promotive function in CE-treated db/db mice, especially in circRNA.5549/miR-29a-5P/Cd36 regulatory network, and this evidence suggest that their ceRNA regulatory network might be a therapeutic target for DN in humans. |
| format | Article |
| id | doaj-art-9459750c7fc7468fa17f505f0376d686 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2022-05-01 |
| publisher | Tsinghua University Press |
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| series | Food Science and Human Wellness |
| spelling | doaj-art-9459750c7fc7468fa17f505f0376d6862025-02-03T05:38:31ZengTsinghua University PressFood Science and Human Wellness2213-45302022-05-01113660668Identification of circRNA/miRNA/mRNA regulatory network involving (+)-catechin ameliorates diabetic nephropathy miceChao Chen0Dina Zhu1Shuai Zhang2Wensheng Zhang3Zhuhai Branch of State Key Laboratory of Earth Surface Processes and Resource Ecology, Advanced Institute of Natural Sciences, Beijing Normal University, Zhuhai 519087, China; Engineering Research Center of Natural Medicine, Ministry of Education, Advanced Institute of Natural Sciences, Beijing Normal University, Zhuhai 519087, ChinaZhuhai Branch of State Key Laboratory of Earth Surface Processes and Resource Ecology, Advanced Institute of Natural Sciences, Beijing Normal University, Zhuhai 519087, China; Engineering Research Center of Natural Medicine, Ministry of Education, Advanced Institute of Natural Sciences, Beijing Normal University, Zhuhai 519087, ChinaInternational Cooperation Laboratory of Molecular Medicine, Academy of Chinese Medical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaZhuhai Branch of State Key Laboratory of Earth Surface Processes and Resource Ecology, Advanced Institute of Natural Sciences, Beijing Normal University, Zhuhai 519087, China; Engineering Research Center of Natural Medicine, Ministry of Education, Advanced Institute of Natural Sciences, Beijing Normal University, Zhuhai 519087, China; Beijing Key Laboratory of Traditional Chinese Medicine Protection and Utilization, Faculty of Geographical Science, Beijing Normal University, Beijing 100875, China; Corresponding author at: Beijing Normal University, Beijing 100875.(+)-Catechin (CE) is mainly found in green and black tea and has many biological effects, such as anti-inflammatory, anti-cancer, anti-viral effects, protecting human organs, especially the kidney. This study aims to identify the circRNAs induced by CE in db/db mice and their roles in diabetic nephropathy progression. After the db/db mice were treated with CE, RNA-seq was performed to identify the differentially expressed circRNA and mRNAs. The ceRNA regulatory network was constructed and analyzed using bioinformatics software and public databases (Cytoscape, ClueGO, MiRWalk, STRING, et al.). Our results revealed that 6 differentially expressed circRNAs are most associated with the cholinergic synapse, neurotrophin signaling pathway, and insulin signaling pathway. Among these, circRNA.5549 and circRNA.4712 might regulate Cd36, Cyp26b1, C8a, Cyp2j13, Grem2 genes through ceRNA regulatory mechanism in the presence of CE treatment. The expanded network of proteins interacting with these 5 genes shows that the TGF-β signaling pathway, signaling pathways regulating pluripotency of stem cell, fat digestion and absorption, and PPAR signaling pathway was highly enriched. Overall, circRNA.5549 and circRNA.4712 exhibit a promotive function in CE-treated db/db mice, especially in circRNA.5549/miR-29a-5P/Cd36 regulatory network, and this evidence suggest that their ceRNA regulatory network might be a therapeutic target for DN in humans.http://www.sciencedirect.com/science/article/pii/S2213453021001518CatechinDiabetic nephropathyCircRNAmiRNAceRNA |
| spellingShingle | Chao Chen Dina Zhu Shuai Zhang Wensheng Zhang Identification of circRNA/miRNA/mRNA regulatory network involving (+)-catechin ameliorates diabetic nephropathy mice Food Science and Human Wellness Catechin Diabetic nephropathy CircRNA miRNA ceRNA |
| title | Identification of circRNA/miRNA/mRNA regulatory network involving (+)-catechin ameliorates diabetic nephropathy mice |
| title_full | Identification of circRNA/miRNA/mRNA regulatory network involving (+)-catechin ameliorates diabetic nephropathy mice |
| title_fullStr | Identification of circRNA/miRNA/mRNA regulatory network involving (+)-catechin ameliorates diabetic nephropathy mice |
| title_full_unstemmed | Identification of circRNA/miRNA/mRNA regulatory network involving (+)-catechin ameliorates diabetic nephropathy mice |
| title_short | Identification of circRNA/miRNA/mRNA regulatory network involving (+)-catechin ameliorates diabetic nephropathy mice |
| title_sort | identification of circrna mirna mrna regulatory network involving catechin ameliorates diabetic nephropathy mice |
| topic | Catechin Diabetic nephropathy CircRNA miRNA ceRNA |
| url | http://www.sciencedirect.com/science/article/pii/S2213453021001518 |
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