Response of the nosZ-type denitrifying microbial community and metabolic characteristics to precipitation changes in the alpine wetland
The impact of climate change on the global hydrological cycle is becoming increasingly significant, with changes in precipitation patterns emerging as a key factor influencing the carbon and nitrogen cycling processes in alpine wetland ecosystems. However, the response of the nosZ-type denitrifying...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-04-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1581432/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850143111848656896 |
|---|---|
| author | Ni Zhang Ni Zhang Ni Zhang Hongchen Jiang Hongchen Jiang Zhiyun Zhou Zhiyun Zhou Zhiyun Zhou Yijun Wang Desheng Qi Desheng Qi Desheng Qi Shijia Zhou Shijia Zhou Shijia Zhou Jing Ma Jing Ma Jing Ma Kelong Chen Kelong Chen Kelong Chen |
| author_facet | Ni Zhang Ni Zhang Ni Zhang Hongchen Jiang Hongchen Jiang Zhiyun Zhou Zhiyun Zhou Zhiyun Zhou Yijun Wang Desheng Qi Desheng Qi Desheng Qi Shijia Zhou Shijia Zhou Shijia Zhou Jing Ma Jing Ma Jing Ma Kelong Chen Kelong Chen Kelong Chen |
| author_sort | Ni Zhang |
| collection | DOAJ |
| description | The impact of climate change on the global hydrological cycle is becoming increasingly significant, with changes in precipitation patterns emerging as a key factor influencing the carbon and nitrogen cycling processes in alpine wetland ecosystems. However, the response of the nosZ-type denitrifying microbial community and its metabolic characteristics in the source wetland to precipitation changes remains unclear. In this study, high-throughput sequencing of the nosZ gene and LC-MS-based metabolomics were used to investigate the response of the nosZ-type denitrifying microbial community and its metabolic characteristics to precipitation changes (4 years) in the source wetland of Qinghai Lake. The results showed that Microvirga was the key bacterial group in the source wetland of Qinghai Lake, and Azospirillum was sensitive to changes in precipitation (P < 0.05). The 50% rainfall enhancement treatment significantly increased soil moisture, and the total carbon content showed an increasing trend with the increase in precipitation (P < 0.05). pH was the most important explanatory factor for community structure, while total nitrogen content was the key explanatory factor for community diversity. Deterministic processes dominated the assemblage of the nosZ-type denitrifying microbial community in the source wetland of Qinghai Lake. Soil metabolomics analysis showed that the differential metabolites in the Source Wetland mostly exhibited significant positive correlations. Precipitation changes significantly affected the relative abundance of N-Acetylaspartic acid. In summary, lower precipitation is more favorable for maintaining carbon storage in the source wetlands of Qinghai Lake. Precipitation variation disrupted the existing nitrogen balance within the ecosystem and altered the structure of the nosZ-type denitrifying microbial community and soil metabolic characteristics. These findings imply that climate change-driven shifts in precipitation patterns may impact carbon and nitrogen dynamics in alpine wetlands, alter ecosystem stability, and have profound effects on microbial communities and biogeochemical cycles. |
| format | Article |
| id | doaj-art-9445e576809d45e284c4f328bddfc77a |
| institution | OA Journals |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-9445e576809d45e284c4f328bddfc77a2025-08-20T02:28:50ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-04-011610.3389/fmicb.2025.15814321581432Response of the nosZ-type denitrifying microbial community and metabolic characteristics to precipitation changes in the alpine wetlandNi Zhang0Ni Zhang1Ni Zhang2Hongchen Jiang3Hongchen Jiang4Zhiyun Zhou5Zhiyun Zhou6Zhiyun Zhou7Yijun Wang8Desheng Qi9Desheng Qi10Desheng Qi11Shijia Zhou12Shijia Zhou13Shijia Zhou14Jing Ma15Jing Ma16Jing Ma17Kelong Chen18Kelong Chen19Kelong Chen20Qinghai Province Key Laboratory of Physical Geography and Environmental Process, College of Geographical Science, Qinghai Normal University, Xining, ChinaKey Laboratory of Tibetan Plateau Land Surface Processes and Ecological Conservation (Ministry of Education), Qinghai Normal University, Xining, ChinaNational Positioning Observation and Research Station of Qinghai Lake Wetland Ecosystem in Qinghai, National Forestry and Grassland Administration, Hubei, Haibei, ChinaState Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, ChinaQinghai Provincial Key Laboratory of Geology and Environment of Salt Lakes, Qinghai Institute of Salt Lakes, Chinese Academy of Sciences, Xining, ChinaQinghai Province Key Laboratory of Physical Geography and Environmental Process, College of Geographical Science, Qinghai Normal University, Xining, ChinaKey Laboratory of Tibetan Plateau Land Surface Processes and Ecological Conservation (Ministry of Education), Qinghai Normal University, Xining, ChinaNational Positioning Observation and Research Station of Qinghai Lake Wetland Ecosystem in Qinghai, National Forestry and Grassland Administration, Hubei, Haibei, ChinaKey Laboratory of Tibetan Plateau Land Surface Processes and Ecological Conservation (Ministry of Education), Qinghai Normal University, Xining, ChinaQinghai Province Key Laboratory of Physical Geography and Environmental Process, College of Geographical Science, Qinghai Normal University, Xining, ChinaKey Laboratory of Tibetan Plateau Land Surface Processes and Ecological Conservation (Ministry of Education), Qinghai Normal University, Xining, ChinaNational Positioning Observation and Research Station of Qinghai Lake Wetland Ecosystem in Qinghai, National Forestry and Grassland Administration, Hubei, Haibei, ChinaQinghai Province Key Laboratory of Physical Geography and Environmental Process, College of Geographical Science, Qinghai Normal University, Xining, ChinaKey Laboratory of Tibetan Plateau Land Surface Processes and Ecological Conservation (Ministry of Education), Qinghai Normal University, Xining, ChinaNational Positioning Observation and Research Station of Qinghai Lake Wetland Ecosystem in Qinghai, National Forestry and Grassland Administration, Hubei, Haibei, ChinaQinghai Province Key Laboratory of Physical Geography and Environmental Process, College of Geographical Science, Qinghai Normal University, Xining, ChinaKey Laboratory of Tibetan Plateau Land Surface Processes and Ecological Conservation (Ministry of Education), Qinghai Normal University, Xining, ChinaNational Positioning Observation and Research Station of Qinghai Lake Wetland Ecosystem in Qinghai, National Forestry and Grassland Administration, Hubei, Haibei, ChinaQinghai Province Key Laboratory of Physical Geography and Environmental Process, College of Geographical Science, Qinghai Normal University, Xining, ChinaKey Laboratory of Tibetan Plateau Land Surface Processes and Ecological Conservation (Ministry of Education), Qinghai Normal University, Xining, ChinaNational Positioning Observation and Research Station of Qinghai Lake Wetland Ecosystem in Qinghai, National Forestry and Grassland Administration, Hubei, Haibei, ChinaThe impact of climate change on the global hydrological cycle is becoming increasingly significant, with changes in precipitation patterns emerging as a key factor influencing the carbon and nitrogen cycling processes in alpine wetland ecosystems. However, the response of the nosZ-type denitrifying microbial community and its metabolic characteristics in the source wetland to precipitation changes remains unclear. In this study, high-throughput sequencing of the nosZ gene and LC-MS-based metabolomics were used to investigate the response of the nosZ-type denitrifying microbial community and its metabolic characteristics to precipitation changes (4 years) in the source wetland of Qinghai Lake. The results showed that Microvirga was the key bacterial group in the source wetland of Qinghai Lake, and Azospirillum was sensitive to changes in precipitation (P < 0.05). The 50% rainfall enhancement treatment significantly increased soil moisture, and the total carbon content showed an increasing trend with the increase in precipitation (P < 0.05). pH was the most important explanatory factor for community structure, while total nitrogen content was the key explanatory factor for community diversity. Deterministic processes dominated the assemblage of the nosZ-type denitrifying microbial community in the source wetland of Qinghai Lake. Soil metabolomics analysis showed that the differential metabolites in the Source Wetland mostly exhibited significant positive correlations. Precipitation changes significantly affected the relative abundance of N-Acetylaspartic acid. In summary, lower precipitation is more favorable for maintaining carbon storage in the source wetlands of Qinghai Lake. Precipitation variation disrupted the existing nitrogen balance within the ecosystem and altered the structure of the nosZ-type denitrifying microbial community and soil metabolic characteristics. These findings imply that climate change-driven shifts in precipitation patterns may impact carbon and nitrogen dynamics in alpine wetlands, alter ecosystem stability, and have profound effects on microbial communities and biogeochemical cycles.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1581432/fullQinghai-Tibetan Plateauclimate changeextreme precipitationcarbon and nitrogen cyclingLC/MS |
| spellingShingle | Ni Zhang Ni Zhang Ni Zhang Hongchen Jiang Hongchen Jiang Zhiyun Zhou Zhiyun Zhou Zhiyun Zhou Yijun Wang Desheng Qi Desheng Qi Desheng Qi Shijia Zhou Shijia Zhou Shijia Zhou Jing Ma Jing Ma Jing Ma Kelong Chen Kelong Chen Kelong Chen Response of the nosZ-type denitrifying microbial community and metabolic characteristics to precipitation changes in the alpine wetland Frontiers in Microbiology Qinghai-Tibetan Plateau climate change extreme precipitation carbon and nitrogen cycling LC/MS |
| title | Response of the nosZ-type denitrifying microbial community and metabolic characteristics to precipitation changes in the alpine wetland |
| title_full | Response of the nosZ-type denitrifying microbial community and metabolic characteristics to precipitation changes in the alpine wetland |
| title_fullStr | Response of the nosZ-type denitrifying microbial community and metabolic characteristics to precipitation changes in the alpine wetland |
| title_full_unstemmed | Response of the nosZ-type denitrifying microbial community and metabolic characteristics to precipitation changes in the alpine wetland |
| title_short | Response of the nosZ-type denitrifying microbial community and metabolic characteristics to precipitation changes in the alpine wetland |
| title_sort | response of the nosz type denitrifying microbial community and metabolic characteristics to precipitation changes in the alpine wetland |
| topic | Qinghai-Tibetan Plateau climate change extreme precipitation carbon and nitrogen cycling LC/MS |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1581432/full |
| work_keys_str_mv | AT nizhang responseofthenosztypedenitrifyingmicrobialcommunityandmetaboliccharacteristicstoprecipitationchangesinthealpinewetland AT nizhang responseofthenosztypedenitrifyingmicrobialcommunityandmetaboliccharacteristicstoprecipitationchangesinthealpinewetland AT nizhang responseofthenosztypedenitrifyingmicrobialcommunityandmetaboliccharacteristicstoprecipitationchangesinthealpinewetland AT hongchenjiang responseofthenosztypedenitrifyingmicrobialcommunityandmetaboliccharacteristicstoprecipitationchangesinthealpinewetland AT hongchenjiang responseofthenosztypedenitrifyingmicrobialcommunityandmetaboliccharacteristicstoprecipitationchangesinthealpinewetland AT zhiyunzhou responseofthenosztypedenitrifyingmicrobialcommunityandmetaboliccharacteristicstoprecipitationchangesinthealpinewetland AT zhiyunzhou responseofthenosztypedenitrifyingmicrobialcommunityandmetaboliccharacteristicstoprecipitationchangesinthealpinewetland AT zhiyunzhou responseofthenosztypedenitrifyingmicrobialcommunityandmetaboliccharacteristicstoprecipitationchangesinthealpinewetland AT yijunwang responseofthenosztypedenitrifyingmicrobialcommunityandmetaboliccharacteristicstoprecipitationchangesinthealpinewetland AT deshengqi responseofthenosztypedenitrifyingmicrobialcommunityandmetaboliccharacteristicstoprecipitationchangesinthealpinewetland AT deshengqi responseofthenosztypedenitrifyingmicrobialcommunityandmetaboliccharacteristicstoprecipitationchangesinthealpinewetland AT deshengqi responseofthenosztypedenitrifyingmicrobialcommunityandmetaboliccharacteristicstoprecipitationchangesinthealpinewetland AT shijiazhou responseofthenosztypedenitrifyingmicrobialcommunityandmetaboliccharacteristicstoprecipitationchangesinthealpinewetland AT shijiazhou responseofthenosztypedenitrifyingmicrobialcommunityandmetaboliccharacteristicstoprecipitationchangesinthealpinewetland AT shijiazhou responseofthenosztypedenitrifyingmicrobialcommunityandmetaboliccharacteristicstoprecipitationchangesinthealpinewetland AT jingma responseofthenosztypedenitrifyingmicrobialcommunityandmetaboliccharacteristicstoprecipitationchangesinthealpinewetland AT jingma responseofthenosztypedenitrifyingmicrobialcommunityandmetaboliccharacteristicstoprecipitationchangesinthealpinewetland AT jingma responseofthenosztypedenitrifyingmicrobialcommunityandmetaboliccharacteristicstoprecipitationchangesinthealpinewetland AT kelongchen responseofthenosztypedenitrifyingmicrobialcommunityandmetaboliccharacteristicstoprecipitationchangesinthealpinewetland AT kelongchen responseofthenosztypedenitrifyingmicrobialcommunityandmetaboliccharacteristicstoprecipitationchangesinthealpinewetland AT kelongchen responseofthenosztypedenitrifyingmicrobialcommunityandmetaboliccharacteristicstoprecipitationchangesinthealpinewetland |