Optimization of factors affecting biogas production from dairy wastewater using Response Surface Methodology and its kinetic and microbial analysis
Anaerobic digestion is one of the most widely used treatment methods for producing methane as an alternative energy source. This study aimed to produce biogas from milk waste via an anaerobic digestion process, which involves high nutrient concentrations and microorganisms. Two batch digesters were...
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Elsevier
2025-06-01
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| Series: | Results in Engineering |
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| author | Sadok Saadia Benmehdi Houcine Baraka Abdelhak Makhloufi Ahmed |
| author_facet | Sadok Saadia Benmehdi Houcine Baraka Abdelhak Makhloufi Ahmed |
| author_sort | Sadok Saadia |
| collection | DOAJ |
| description | Anaerobic digestion is one of the most widely used treatment methods for producing methane as an alternative energy source. This study aimed to produce biogas from milk waste via an anaerobic digestion process, which involves high nutrient concentrations and microorganisms. Two batch digesters were designed to be capable of producing biogas under the experimental protocol, and the highest daily gas yield was monitored for 64 days. In this context, we conducted an experimental study using the design of experiments (DOE) method for the optimization of biogas production and determined the ideal conditions for biogas generation, which depend on the temperature, humidity, pH, and retention time. The design used in response surface methodology (RSM) studies is a central composite design (MODDE 5.0 software). The various factors affecting anaerobic digestion were optimized with the following parameters: temperature T = 39 °C, pH = 7.45, moisture content 90 % and retention time 59 h. The percentages of nutrients before digestion were 70.6 % organic matter, 40.94 %, 2.85 mg/L and 5.56 % total organic carbon, phosphorus and nitrogen, respectively. The total volume of biogas formed was 17.206 L. The microbiological analysis focused on six kinds of bacteria: mesophilic anaerobic flora, mesophilic aerobic flora, coliforms, Staphylococcus, Pseudomonas and fungal flora. The results of this study indicate that biotechnological processes are adequate for treating milk waste to produce important products such as good-quality methane, which is very rich in methane (49.6 %). |
| format | Article |
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| institution | OA Journals |
| issn | 2590-1230 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
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| series | Results in Engineering |
| spelling | doaj-art-943ddfffcb3444f1b1770cebbd79ba4b2025-08-20T02:12:16ZengElsevierResults in Engineering2590-12302025-06-012610478110.1016/j.rineng.2025.104781Optimization of factors affecting biogas production from dairy wastewater using Response Surface Methodology and its kinetic and microbial analysisSadok Saadia0Benmehdi Houcine1Baraka Abdelhak2Makhloufi Ahmed3Laboratory of Chemistry and Environmental Sciences, Faculty of Technology, University of Bechar Tahri Mohammed, P.O. Box 417, Bechar 08000, Algeria; Corresponding author.Laboratory of Chemistry and Environmental Sciences, Faculty of Technology, University of Bechar Tahri Mohammed, P.O. Box 417, Bechar 08000, AlgeriaEMIA (Eco-Materials Innovation & Application) Laboratory, University of Bechar Tahri Mohammed, P.O. Box 417, Bechar 08000, AlgeriaLaboratory of Valorization of Vegetal Resource and Food Security in Semi-arid Areas, South West of Algeria, University of Bechar Tahri Mohammed, P.O. Box 417, Bechar 08000, AlgeriaAnaerobic digestion is one of the most widely used treatment methods for producing methane as an alternative energy source. This study aimed to produce biogas from milk waste via an anaerobic digestion process, which involves high nutrient concentrations and microorganisms. Two batch digesters were designed to be capable of producing biogas under the experimental protocol, and the highest daily gas yield was monitored for 64 days. In this context, we conducted an experimental study using the design of experiments (DOE) method for the optimization of biogas production and determined the ideal conditions for biogas generation, which depend on the temperature, humidity, pH, and retention time. The design used in response surface methodology (RSM) studies is a central composite design (MODDE 5.0 software). The various factors affecting anaerobic digestion were optimized with the following parameters: temperature T = 39 °C, pH = 7.45, moisture content 90 % and retention time 59 h. The percentages of nutrients before digestion were 70.6 % organic matter, 40.94 %, 2.85 mg/L and 5.56 % total organic carbon, phosphorus and nitrogen, respectively. The total volume of biogas formed was 17.206 L. The microbiological analysis focused on six kinds of bacteria: mesophilic anaerobic flora, mesophilic aerobic flora, coliforms, Staphylococcus, Pseudomonas and fungal flora. The results of this study indicate that biotechnological processes are adequate for treating milk waste to produce important products such as good-quality methane, which is very rich in methane (49.6 %).http://www.sciencedirect.com/science/article/pii/S2590123025008588Anaerobic digestionBiogasMilk wasteNutrients |
| spellingShingle | Sadok Saadia Benmehdi Houcine Baraka Abdelhak Makhloufi Ahmed Optimization of factors affecting biogas production from dairy wastewater using Response Surface Methodology and its kinetic and microbial analysis Results in Engineering Anaerobic digestion Biogas Milk waste Nutrients |
| title | Optimization of factors affecting biogas production from dairy wastewater using Response Surface Methodology and its kinetic and microbial analysis |
| title_full | Optimization of factors affecting biogas production from dairy wastewater using Response Surface Methodology and its kinetic and microbial analysis |
| title_fullStr | Optimization of factors affecting biogas production from dairy wastewater using Response Surface Methodology and its kinetic and microbial analysis |
| title_full_unstemmed | Optimization of factors affecting biogas production from dairy wastewater using Response Surface Methodology and its kinetic and microbial analysis |
| title_short | Optimization of factors affecting biogas production from dairy wastewater using Response Surface Methodology and its kinetic and microbial analysis |
| title_sort | optimization of factors affecting biogas production from dairy wastewater using response surface methodology and its kinetic and microbial analysis |
| topic | Anaerobic digestion Biogas Milk waste Nutrients |
| url | http://www.sciencedirect.com/science/article/pii/S2590123025008588 |
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