Demodulation and purification of ellagic acid from pomegranate fruit and study antimicrobial activity against the pathogenic bacteria

The ellagic acid is isolated from  pomegranate fruit by liquid–liquid extraction method. This method includes extraction  with 20% (v/v) methanol under reflux conditions for  purification of acid by preparative TLC method using glass plates (20 × 20 cm) coated with silica gel .This acid was detecte...

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Main Authors: Hiba H. R, Eesa. M. Thalij, Sundus J. M.
Format: Article
Language:English
Published: Tikrit University 2019-11-01
Series:Tikrit Journal of Pure Science
Subjects:
Online Access:https://tjpsj.org/index.php/tjps/article/view/435
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author Hiba H. R
Eesa. M. Thalij
Sundus J. M.
author_facet Hiba H. R
Eesa. M. Thalij
Sundus J. M.
author_sort Hiba H. R
collection DOAJ
description The ellagic acid is isolated from  pomegranate fruit by liquid–liquid extraction method. This method includes extraction  with 20% (v/v) methanol under reflux conditions for  purification of acid by preparative TLC method using glass plates (20 × 20 cm) coated with silica gel .This acid was detected by TLC, HPLC, UV methods and functional group of the acidwhich was extracted by FTIR technique. The antibacterial activity of Ellagic acid extract of pomegranate fruit  powder was defined by petri Dish technique versus different microbial types, Gram-positive (Staphylococcus aureus), Gram-negative (Escherichia coli, Pseudomonas aeruginosa) and two Yeasts(C. glabrata and C. albicams).
format Article
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institution Kabale University
issn 1813-1662
2415-1726
language English
publishDate 2019-11-01
publisher Tikrit University
record_format Article
series Tikrit Journal of Pure Science
spelling doaj-art-943ba07eee704711b7ae853a2bc735e72025-08-20T03:34:26ZengTikrit UniversityTikrit Journal of Pure Science1813-16622415-17262019-11-0124610.25130/tjps.v24i6.435Demodulation and purification of ellagic acid from pomegranate fruit and study antimicrobial activity against the pathogenic bacteriaHiba H. REesa. M. ThalijSundus J. M. The ellagic acid is isolated from  pomegranate fruit by liquid–liquid extraction method. This method includes extraction  with 20% (v/v) methanol under reflux conditions for  purification of acid by preparative TLC method using glass plates (20 × 20 cm) coated with silica gel .This acid was detected by TLC, HPLC, UV methods and functional group of the acidwhich was extracted by FTIR technique. The antibacterial activity of Ellagic acid extract of pomegranate fruit  powder was defined by petri Dish technique versus different microbial types, Gram-positive (Staphylococcus aureus), Gram-negative (Escherichia coli, Pseudomonas aeruginosa) and two Yeasts(C. glabrata and C. albicams). https://tjpsj.org/index.php/tjps/article/view/435Ellagic acidliquid–liquid extractionFT-IRpomegranate fruit
spellingShingle Hiba H. R
Eesa. M. Thalij
Sundus J. M.
Demodulation and purification of ellagic acid from pomegranate fruit and study antimicrobial activity against the pathogenic bacteria
Tikrit Journal of Pure Science
Ellagic acid
liquid–liquid extraction
FT-IR
pomegranate fruit
title Demodulation and purification of ellagic acid from pomegranate fruit and study antimicrobial activity against the pathogenic bacteria
title_full Demodulation and purification of ellagic acid from pomegranate fruit and study antimicrobial activity against the pathogenic bacteria
title_fullStr Demodulation and purification of ellagic acid from pomegranate fruit and study antimicrobial activity against the pathogenic bacteria
title_full_unstemmed Demodulation and purification of ellagic acid from pomegranate fruit and study antimicrobial activity against the pathogenic bacteria
title_short Demodulation and purification of ellagic acid from pomegranate fruit and study antimicrobial activity against the pathogenic bacteria
title_sort demodulation and purification of ellagic acid from pomegranate fruit and study antimicrobial activity against the pathogenic bacteria
topic Ellagic acid
liquid–liquid extraction
FT-IR
pomegranate fruit
url https://tjpsj.org/index.php/tjps/article/view/435
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