Zr‐MOF Carrier‐Enhanced Dual‐Mode Biosensing Platforms for Rapid and Sensitive Diagnosis of Mpox
Abstract Dual‐mode readout platforms with colorimetric and electrochemiluminescence (ECL) signal enhancement are proposed for the ultrasensitive and flexible detection of the monkeypox virus (MPXV) in different scenes. A new nanotag, Ru@U6‐Ru/Pt NPs is constructed for dual‐mode platforms by integrat...
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| Format: | Article |
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Wiley
2024-10-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202405848 |
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| author | Huiyi Yang Judun Zheng Wei Wang Jingyan Lin Jingru Wang Lunjing Liu Wenjie Wu Chengli Zhang Mingxia Zhang Yu Fu Bin Yang Yuhui Liao |
| author_facet | Huiyi Yang Judun Zheng Wei Wang Jingyan Lin Jingru Wang Lunjing Liu Wenjie Wu Chengli Zhang Mingxia Zhang Yu Fu Bin Yang Yuhui Liao |
| author_sort | Huiyi Yang |
| collection | DOAJ |
| description | Abstract Dual‐mode readout platforms with colorimetric and electrochemiluminescence (ECL) signal enhancement are proposed for the ultrasensitive and flexible detection of the monkeypox virus (MPXV) in different scenes. A new nanotag, Ru@U6‐Ru/Pt NPs is constructed for dual‐mode platforms by integrating double‐layered ECL luminophores and the nanozyme using Zr‐MOF (UiO‐66‐NH2) as the carrier, which not only generates enhanced ECL and colorimetric signals but also provide greater stability than that of commonly used nanotags. Dual‐mode platforms are used within 15 min from the “sample in” to the “result out” steps, without nucleic acid amplification. The colorimetric mode allows the screening of MPXV with the visual limit of detection (vLOD) of 0.1 pM (6 × 108 copies µL−1) and the ECL mode supports quantitative detection of MPXV with an LOD as low as 10 aM (6 copies·µL−1), resulting in a broad sensing range of 60 to 3 × 1011 copies·µL−1 (10 orders of magnitude). Validation is conducted using 50 clinical samples, which is 100% concordant to those of quantitative polymerase chain reaction (qPCR), indicating that Ru@U6‐Ru/Pt NPs‐based dual‐mode sensing platforms showed great promise as rapid, sensitive, and accurate tools for diagnosis of the nucleic acid of MPXV and other infectious pathogens. |
| format | Article |
| id | doaj-art-94364a825e9f4e71962e979b52eccadc |
| institution | OA Journals |
| issn | 2198-3844 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-94364a825e9f4e71962e979b52eccadc2025-08-20T02:17:10ZengWileyAdvanced Science2198-38442024-10-011138n/an/a10.1002/advs.202405848Zr‐MOF Carrier‐Enhanced Dual‐Mode Biosensing Platforms for Rapid and Sensitive Diagnosis of MpoxHuiyi Yang0Judun Zheng1Wei Wang2Jingyan Lin3Jingru Wang4Lunjing Liu5Wenjie Wu6Chengli Zhang7Mingxia Zhang8Yu Fu9Bin Yang10Yuhui Liao11Molecular Diagnosis and Treatment Center for Infectious Diseases Dermatology Hospital Southern Medical University Guangzhou 510000 ChinaMolecular Diagnosis and Treatment Center for Infectious Diseases Dermatology Hospital Southern Medical University Guangzhou 510000 ChinaInstitute for Engineering Medicine Kunming Medical University Kunming 650500 ChinaNational Clinical Research Center for Infectious Disease The Second Affiliated Hospital of Southern University of Science and Technology Shenzhen Third People's Hospital Shenzhen 518000 ChinaInstitute for Engineering Medicine Kunming Medical University Kunming 650500 ChinaNHC Key Laboratory of Metabolic Cardiovascular Diseases Research Ningxia Key Laboratory of Vascular Injury and Repair Research Ningxia Medical University Yinchuan 750004 ChinaInstitute for Engineering Medicine Kunming Medical University Kunming 650500 ChinaMolecular Diagnosis and Treatment Center for Infectious Diseases Dermatology Hospital Southern Medical University Guangzhou 510000 ChinaNational Clinical Research Center for Infectious Disease The Second Affiliated Hospital of Southern University of Science and Technology Shenzhen Third People's Hospital Shenzhen 518000 ChinaNHC Key Laboratory of Metabolic Cardiovascular Diseases Research Ningxia Key Laboratory of Vascular Injury and Repair Research Ningxia Medical University Yinchuan 750004 ChinaMolecular Diagnosis and Treatment Center for Infectious Diseases Dermatology Hospital Southern Medical University Guangzhou 510000 ChinaInstitute for Engineering Medicine Kunming Medical University Kunming 650500 ChinaAbstract Dual‐mode readout platforms with colorimetric and electrochemiluminescence (ECL) signal enhancement are proposed for the ultrasensitive and flexible detection of the monkeypox virus (MPXV) in different scenes. A new nanotag, Ru@U6‐Ru/Pt NPs is constructed for dual‐mode platforms by integrating double‐layered ECL luminophores and the nanozyme using Zr‐MOF (UiO‐66‐NH2) as the carrier, which not only generates enhanced ECL and colorimetric signals but also provide greater stability than that of commonly used nanotags. Dual‐mode platforms are used within 15 min from the “sample in” to the “result out” steps, without nucleic acid amplification. The colorimetric mode allows the screening of MPXV with the visual limit of detection (vLOD) of 0.1 pM (6 × 108 copies µL−1) and the ECL mode supports quantitative detection of MPXV with an LOD as low as 10 aM (6 copies·µL−1), resulting in a broad sensing range of 60 to 3 × 1011 copies·µL−1 (10 orders of magnitude). Validation is conducted using 50 clinical samples, which is 100% concordant to those of quantitative polymerase chain reaction (qPCR), indicating that Ru@U6‐Ru/Pt NPs‐based dual‐mode sensing platforms showed great promise as rapid, sensitive, and accurate tools for diagnosis of the nucleic acid of MPXV and other infectious pathogens.https://doi.org/10.1002/advs.202405848amplification‐freecolorimetric‐ECL dual‐modemonkeypox virusRu@U6‐Ru/Pt NPs nanotagsignal amplification |
| spellingShingle | Huiyi Yang Judun Zheng Wei Wang Jingyan Lin Jingru Wang Lunjing Liu Wenjie Wu Chengli Zhang Mingxia Zhang Yu Fu Bin Yang Yuhui Liao Zr‐MOF Carrier‐Enhanced Dual‐Mode Biosensing Platforms for Rapid and Sensitive Diagnosis of Mpox Advanced Science amplification‐free colorimetric‐ECL dual‐mode monkeypox virus Ru@U6‐Ru/Pt NPs nanotag signal amplification |
| title | Zr‐MOF Carrier‐Enhanced Dual‐Mode Biosensing Platforms for Rapid and Sensitive Diagnosis of Mpox |
| title_full | Zr‐MOF Carrier‐Enhanced Dual‐Mode Biosensing Platforms for Rapid and Sensitive Diagnosis of Mpox |
| title_fullStr | Zr‐MOF Carrier‐Enhanced Dual‐Mode Biosensing Platforms for Rapid and Sensitive Diagnosis of Mpox |
| title_full_unstemmed | Zr‐MOF Carrier‐Enhanced Dual‐Mode Biosensing Platforms for Rapid and Sensitive Diagnosis of Mpox |
| title_short | Zr‐MOF Carrier‐Enhanced Dual‐Mode Biosensing Platforms for Rapid and Sensitive Diagnosis of Mpox |
| title_sort | zr mof carrier enhanced dual mode biosensing platforms for rapid and sensitive diagnosis of mpox |
| topic | amplification‐free colorimetric‐ECL dual‐mode monkeypox virus Ru@U6‐Ru/Pt NPs nanotag signal amplification |
| url | https://doi.org/10.1002/advs.202405848 |
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