Research Progresses in Vitrification by Minimum Volume Method
Vitrification is an effective method in the field of cryopreservation since it can prevent the formation of ice crystals. Vitrification requires ultra-fast cooling rates and high concentrations of cryoprotective agents. However, osmotic injury and toxic damage can be caused by high concentration of...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
Journal of Refrigeration Magazines Agency Co., Ltd.
2018-01-01
|
| Series: | Zhileng xuebao |
| Subjects: | |
| Online Access: | http://www.zhilengxuebao.com/thesisDetails#10.3969/j.issn.0253-4339.2018.04.063 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849705342005411840 |
|---|---|
| author | Zhang Xiaomin Zhou Xinli |
| author_facet | Zhang Xiaomin Zhou Xinli |
| author_sort | Zhang Xiaomin |
| collection | DOAJ |
| description | Vitrification is an effective method in the field of cryopreservation since it can prevent the formation of ice crystals. Vitrification requires ultra-fast cooling rates and high concentrations of cryoprotective agents. However, osmotic injury and toxic damage can be caused by high concentration of cryoprotective agents. The minimum volume methods can significantly increase the cooling rate and reduce the concentration of cryoprotective agents in vitrification. In this paper, the latest research progresses in vitrification by minimum volume methods are reviewed. The minimum volume methods include carrier-based droplet vitrification, spray droplet vitrification, bio-printing droplet vitrification, and microfluidic encapsulation droplet vitrification. Among these, spray droplet vitrification, bio-printing droplet vitrification, and microfluidic encapsulation droplet vitrification are the combination of emerging droplet generation technology and vitrification technology. Because of the rapid generation of uniform droplets and high throughput, they have great potential in the field of cryopreservation. |
| format | Article |
| id | doaj-art-943573f47daf4f6da91ce48410adb0fd |
| institution | DOAJ |
| issn | 0253-4339 |
| language | zho |
| publishDate | 2018-01-01 |
| publisher | Journal of Refrigeration Magazines Agency Co., Ltd. |
| record_format | Article |
| series | Zhileng xuebao |
| spelling | doaj-art-943573f47daf4f6da91ce48410adb0fd2025-08-20T03:16:29ZzhoJournal of Refrigeration Magazines Agency Co., Ltd.Zhileng xuebao0253-43392018-01-013966512583Research Progresses in Vitrification by Minimum Volume MethodZhang XiaominZhou XinliVitrification is an effective method in the field of cryopreservation since it can prevent the formation of ice crystals. Vitrification requires ultra-fast cooling rates and high concentrations of cryoprotective agents. However, osmotic injury and toxic damage can be caused by high concentration of cryoprotective agents. The minimum volume methods can significantly increase the cooling rate and reduce the concentration of cryoprotective agents in vitrification. In this paper, the latest research progresses in vitrification by minimum volume methods are reviewed. The minimum volume methods include carrier-based droplet vitrification, spray droplet vitrification, bio-printing droplet vitrification, and microfluidic encapsulation droplet vitrification. Among these, spray droplet vitrification, bio-printing droplet vitrification, and microfluidic encapsulation droplet vitrification are the combination of emerging droplet generation technology and vitrification technology. Because of the rapid generation of uniform droplets and high throughput, they have great potential in the field of cryopreservation.http://www.zhilengxuebao.com/thesisDetails#10.3969/j.issn.0253-4339.2018.04.063minimum volume methodsvitrificationspray dropletbio-printingmicrofluidic encapsulation |
| spellingShingle | Zhang Xiaomin Zhou Xinli Research Progresses in Vitrification by Minimum Volume Method Zhileng xuebao minimum volume methods vitrification spray droplet bio-printing microfluidic encapsulation |
| title | Research Progresses in Vitrification by Minimum Volume Method |
| title_full | Research Progresses in Vitrification by Minimum Volume Method |
| title_fullStr | Research Progresses in Vitrification by Minimum Volume Method |
| title_full_unstemmed | Research Progresses in Vitrification by Minimum Volume Method |
| title_short | Research Progresses in Vitrification by Minimum Volume Method |
| title_sort | research progresses in vitrification by minimum volume method |
| topic | minimum volume methods vitrification spray droplet bio-printing microfluidic encapsulation |
| url | http://www.zhilengxuebao.com/thesisDetails#10.3969/j.issn.0253-4339.2018.04.063 |
| work_keys_str_mv | AT zhangxiaomin researchprogressesinvitrificationbyminimumvolumemethod AT zhouxinli researchprogressesinvitrificationbyminimumvolumemethod |