<span style="font-variant: small-caps">d</span>-Xylitol Production from Sugar Beet Press Pulp Hydrolysate with Engineered <i>Aspergillus niger</i>
<span style="font-variant: small-caps;">d</span>-Xylitol is a low-calorie and anti-cariogenic sweetener suitable for diabetic patients, making it a valuable ingredient in various health-related applications. In this study, we investigated the production of <span style="...
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MDPI AG
2024-12-01
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| Online Access: | https://www.mdpi.com/2076-2607/12/12/2489 |
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| author | Melanie Knesebeck Marcel Rüllke Veronika Schönrock J. Philipp Benz Dirk Weuster-Botz |
| author_facet | Melanie Knesebeck Marcel Rüllke Veronika Schönrock J. Philipp Benz Dirk Weuster-Botz |
| author_sort | Melanie Knesebeck |
| collection | DOAJ |
| description | <span style="font-variant: small-caps;">d</span>-Xylitol is a low-calorie and anti-cariogenic sweetener suitable for diabetic patients, making it a valuable ingredient in various health-related applications. In this study, we investigated the production of <span style="font-variant: small-caps;">d</span>-xylitol from<span style="font-variant: small-caps;"> l</span>-arabinose derived from sugar beet press pulp (SBPP) hydrolysate using an engineered <i>Aspergillus niger</i> strain. Initial batch studies applying stirred tank bioreactors demonstrated <span style="font-variant: small-caps;">d</span>-xylitol production of 4.6 g L<sup>−1</sup> with a yield of 0.37 g <span style="font-variant: small-caps;">d</span>-xylitol g<sup>−1</sup> <span style="font-variant: small-caps;">l</span>-arabinose with a synthetic medium. Subsequently, the conversion of enzymatically produced and clarified SBPP hydrolysate was studied. We found that pre-treatment of the enzymatic hydrolysate with activated carbon was essential to remove inhibitory components. Moreover, an automated aeration switch-off was implemented based on the CO<sub>2</sub> signal of the off-gas analyzer of the stirred tank bioreactor to prevent <span style="font-variant: small-caps;">d</span>-xylitol degradation after <span style="font-variant: small-caps;">l</span>-arabinose depletion. This resulted in a final <span style="font-variant: small-caps;">d</span>-xylitol concentration of 4.3 g L<sup>−1</sup> with an improved yield of 0.43 g <span style="font-variant: small-caps;">d</span>-xylitol g<sup>−1</sup> <span style="font-variant: small-caps;">l</span>-arabinose. The feasibility of utilizing the agricultural residue SBPP for <span style="font-variant: small-caps;">d</span>-xylitol production was successfully demonstrated with engineered <i>A. niger</i>. |
| format | Article |
| id | doaj-art-94260af7c90c4cc3aa8a2b43708bdef0 |
| institution | DOAJ |
| issn | 2076-2607 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj-art-94260af7c90c4cc3aa8a2b43708bdef02025-08-20T02:51:07ZengMDPI AGMicroorganisms2076-26072024-12-011212248910.3390/microorganisms12122489<span style="font-variant: small-caps">d</span>-Xylitol Production from Sugar Beet Press Pulp Hydrolysate with Engineered <i>Aspergillus niger</i>Melanie Knesebeck0Marcel Rüllke1Veronika Schönrock2J. Philipp Benz3Dirk Weuster-Botz4Biochemical Engineering, TUM School of Engineering and Design, Technical University of Munich, Boltzmannstraße 15, 85748 Garching, GermanyFungal Biotechnology in Wood Science, TUM School of Life Sciences, Technical University of Munich, Hans-Carl-von-Carlowitz-Platz 2, 85354 Freising, GermanyFungal Biotechnology in Wood Science, TUM School of Life Sciences, Technical University of Munich, Hans-Carl-von-Carlowitz-Platz 2, 85354 Freising, GermanyFungal Biotechnology in Wood Science, TUM School of Life Sciences, Technical University of Munich, Hans-Carl-von-Carlowitz-Platz 2, 85354 Freising, GermanyBiochemical Engineering, TUM School of Engineering and Design, Technical University of Munich, Boltzmannstraße 15, 85748 Garching, Germany<span style="font-variant: small-caps;">d</span>-Xylitol is a low-calorie and anti-cariogenic sweetener suitable for diabetic patients, making it a valuable ingredient in various health-related applications. In this study, we investigated the production of <span style="font-variant: small-caps;">d</span>-xylitol from<span style="font-variant: small-caps;"> l</span>-arabinose derived from sugar beet press pulp (SBPP) hydrolysate using an engineered <i>Aspergillus niger</i> strain. Initial batch studies applying stirred tank bioreactors demonstrated <span style="font-variant: small-caps;">d</span>-xylitol production of 4.6 g L<sup>−1</sup> with a yield of 0.37 g <span style="font-variant: small-caps;">d</span>-xylitol g<sup>−1</sup> <span style="font-variant: small-caps;">l</span>-arabinose with a synthetic medium. Subsequently, the conversion of enzymatically produced and clarified SBPP hydrolysate was studied. We found that pre-treatment of the enzymatic hydrolysate with activated carbon was essential to remove inhibitory components. Moreover, an automated aeration switch-off was implemented based on the CO<sub>2</sub> signal of the off-gas analyzer of the stirred tank bioreactor to prevent <span style="font-variant: small-caps;">d</span>-xylitol degradation after <span style="font-variant: small-caps;">l</span>-arabinose depletion. This resulted in a final <span style="font-variant: small-caps;">d</span>-xylitol concentration of 4.3 g L<sup>−1</sup> with an improved yield of 0.43 g <span style="font-variant: small-caps;">d</span>-xylitol g<sup>−1</sup> <span style="font-variant: small-caps;">l</span>-arabinose. The feasibility of utilizing the agricultural residue SBPP for <span style="font-variant: small-caps;">d</span>-xylitol production was successfully demonstrated with engineered <i>A. niger</i>.https://www.mdpi.com/2076-2607/12/12/2489<span style="font-variant: small-caps">d</span>-xylitol production<i>Aspergillus niger</i> NRRL3sugar beet press pulp hydrolysate<span style="font-variant: small-caps">l</span>-arabinosebioconversion |
| spellingShingle | Melanie Knesebeck Marcel Rüllke Veronika Schönrock J. Philipp Benz Dirk Weuster-Botz <span style="font-variant: small-caps">d</span>-Xylitol Production from Sugar Beet Press Pulp Hydrolysate with Engineered <i>Aspergillus niger</i> Microorganisms <span style="font-variant: small-caps">d</span>-xylitol production <i>Aspergillus niger</i> NRRL3 sugar beet press pulp hydrolysate <span style="font-variant: small-caps">l</span>-arabinose bioconversion |
| title | <span style="font-variant: small-caps">d</span>-Xylitol Production from Sugar Beet Press Pulp Hydrolysate with Engineered <i>Aspergillus niger</i> |
| title_full | <span style="font-variant: small-caps">d</span>-Xylitol Production from Sugar Beet Press Pulp Hydrolysate with Engineered <i>Aspergillus niger</i> |
| title_fullStr | <span style="font-variant: small-caps">d</span>-Xylitol Production from Sugar Beet Press Pulp Hydrolysate with Engineered <i>Aspergillus niger</i> |
| title_full_unstemmed | <span style="font-variant: small-caps">d</span>-Xylitol Production from Sugar Beet Press Pulp Hydrolysate with Engineered <i>Aspergillus niger</i> |
| title_short | <span style="font-variant: small-caps">d</span>-Xylitol Production from Sugar Beet Press Pulp Hydrolysate with Engineered <i>Aspergillus niger</i> |
| title_sort | span style font variant small caps d span xylitol production from sugar beet press pulp hydrolysate with engineered i aspergillus niger i |
| topic | <span style="font-variant: small-caps">d</span>-xylitol production <i>Aspergillus niger</i> NRRL3 sugar beet press pulp hydrolysate <span style="font-variant: small-caps">l</span>-arabinose bioconversion |
| url | https://www.mdpi.com/2076-2607/12/12/2489 |
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