Pseudomonas aeruginosa Detection Using Conventional PCR and Quantitative Real-Time PCR Based on Species-Specific Novel Gene Targets Identified by Pangenome Analysis

Pseudomonas aeruginosa Detection Using Conventional PCR and Quantitative Real-Time PCR Based on Species-Specific Novel Gene Targets Identified by Pangenome Analysis

Mining novel specific molecular targets and establishing efficient identification methods are significant for detecting Pseudomonas aeruginosa, which can enable P. aeruginosa tracing in food and water. Pangenome analysis was used to analyze the whole genomic sequences of 2017 strains (including 1,00...

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Bibliographic Details
Main Authors: Chufang Wang, Qinghua Ye, Aiming Jiang, Jumei Zhang, Yuting Shang, Fan Li, Baoqing Zhou, Xinran Xiang, Qihui Gu, Rui Pang, Yu Ding, Shi Wu, Moutong Chen, Qingping Wu, Juan Wang
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-05-01
Series:Frontiers in Microbiology
Subjects:
novel target gene
Pseudomonas aeruginosa
pangenome analysis
PCR
ready-to-eat vegetables
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2022.820431/full
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