MMP-Activated Fluorescence Imaging Detects Early Joint Inflammation in Collagen-Antibody-Induced Arthritis in CC-Chemokine Receptor-2-Null Mice, In-Vivo
The Standard measures of experimental arthritis fail to detect, visualize, and quantify early inflammation and disease activity. Here, we describe the use of an injectable MMP-activated fluorescence agent for in vivo quantification of acute inflammation produced by collagen-antibody-induced arthrit...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | International Journal of Inflammation |
| Online Access: | http://dx.doi.org/10.4061/2011/691587 |
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| _version_ | 1832556078205763584 |
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| author | Jessica M. Ibarra Fabio Jimenez Hernan G. Martinez Kassandra Clark Seema S. Ahuja |
| author_facet | Jessica M. Ibarra Fabio Jimenez Hernan G. Martinez Kassandra Clark Seema S. Ahuja |
| author_sort | Jessica M. Ibarra |
| collection | DOAJ |
| description | The Standard measures of experimental arthritis fail to detect, visualize, and quantify early inflammation and disease activity. Here, we describe the use of an injectable MMP-activated fluorescence agent for in vivo quantification of acute inflammation produced by collagen-antibody-induced arthritis (CAIA) in CC chemokine receptor-2 (Ccr2−/−) null mice. Although Ccr2−/− DBA1/J mice were highly susceptible to and rapidly developed CAIA, the standard clinical assessment of fore or hind paw thicknesses was unable to detect significant acute inflammatory changes (days 3–10). Remarkably, noninvasive, in situ, MMP-activatable fluorescent imaging of Ccr2−/− DBA1/J mice with CAIA displayed acute joint pathology in advance of clinically measurable acute inflammation (days 5, 7, and 10). These results were confirmed by the histology of ankle joints, which showed significant inflammation, bone loss, and synovial hyperplasia, compared to control mice at postimmunization day 5. The MMP-mediated fluorescence technique holds tremendous implications for quantifiable examination of arthritis disease activity of acute joint inflammation. |
| format | Article |
| id | doaj-art-941f22f4a8084a34a27d0a924cda71f2 |
| institution | Kabale University |
| issn | 2042-0099 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Inflammation |
| spelling | doaj-art-941f22f4a8084a34a27d0a924cda71f22025-02-03T05:46:26ZengWileyInternational Journal of Inflammation2042-00992011-01-01201110.4061/2011/691587691587MMP-Activated Fluorescence Imaging Detects Early Joint Inflammation in Collagen-Antibody-Induced Arthritis in CC-Chemokine Receptor-2-Null Mice, In-VivoJessica M. Ibarra0Fabio Jimenez1Hernan G. Martinez2Kassandra Clark3Seema S. Ahuja4Department of Medicine, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229-3900, USADepartment of Medicine, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229-3900, USADepartment of Medicine, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229-3900, USADepartment of Medicine, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229-3900, USADepartment of Medicine, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229-3900, USAThe Standard measures of experimental arthritis fail to detect, visualize, and quantify early inflammation and disease activity. Here, we describe the use of an injectable MMP-activated fluorescence agent for in vivo quantification of acute inflammation produced by collagen-antibody-induced arthritis (CAIA) in CC chemokine receptor-2 (Ccr2−/−) null mice. Although Ccr2−/− DBA1/J mice were highly susceptible to and rapidly developed CAIA, the standard clinical assessment of fore or hind paw thicknesses was unable to detect significant acute inflammatory changes (days 3–10). Remarkably, noninvasive, in situ, MMP-activatable fluorescent imaging of Ccr2−/− DBA1/J mice with CAIA displayed acute joint pathology in advance of clinically measurable acute inflammation (days 5, 7, and 10). These results were confirmed by the histology of ankle joints, which showed significant inflammation, bone loss, and synovial hyperplasia, compared to control mice at postimmunization day 5. The MMP-mediated fluorescence technique holds tremendous implications for quantifiable examination of arthritis disease activity of acute joint inflammation.http://dx.doi.org/10.4061/2011/691587 |
| spellingShingle | Jessica M. Ibarra Fabio Jimenez Hernan G. Martinez Kassandra Clark Seema S. Ahuja MMP-Activated Fluorescence Imaging Detects Early Joint Inflammation in Collagen-Antibody-Induced Arthritis in CC-Chemokine Receptor-2-Null Mice, In-Vivo International Journal of Inflammation |
| title | MMP-Activated Fluorescence Imaging Detects Early Joint Inflammation in Collagen-Antibody-Induced Arthritis in CC-Chemokine Receptor-2-Null Mice, In-Vivo |
| title_full | MMP-Activated Fluorescence Imaging Detects Early Joint Inflammation in Collagen-Antibody-Induced Arthritis in CC-Chemokine Receptor-2-Null Mice, In-Vivo |
| title_fullStr | MMP-Activated Fluorescence Imaging Detects Early Joint Inflammation in Collagen-Antibody-Induced Arthritis in CC-Chemokine Receptor-2-Null Mice, In-Vivo |
| title_full_unstemmed | MMP-Activated Fluorescence Imaging Detects Early Joint Inflammation in Collagen-Antibody-Induced Arthritis in CC-Chemokine Receptor-2-Null Mice, In-Vivo |
| title_short | MMP-Activated Fluorescence Imaging Detects Early Joint Inflammation in Collagen-Antibody-Induced Arthritis in CC-Chemokine Receptor-2-Null Mice, In-Vivo |
| title_sort | mmp activated fluorescence imaging detects early joint inflammation in collagen antibody induced arthritis in cc chemokine receptor 2 null mice in vivo |
| url | http://dx.doi.org/10.4061/2011/691587 |
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