Mechanism of engineered macrophage membrane bionic gene-carrying nanospheres for targeted drug delivery to promote wound repair in deep second-degree burns
Abstract Hepatocyte growth factor (HGF) is a substance that stimulates the proliferation of hepatocytes which promote healing. We developed a macrophage membrane-encapsulated nanosphere drug delivery system containing HGF for the study of burn wound healing. Twenty-seven Sprague–Dawley rats were ran...
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2025-01-01
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| author | Zhihan Zhu Xinghua Zhu Shichen Miao Bolin Wang Zihan Li Dinghao Zhang Shentian Zou Yi Zhang Qingrong Zhang Kesu Hu |
| author_facet | Zhihan Zhu Xinghua Zhu Shichen Miao Bolin Wang Zihan Li Dinghao Zhang Shentian Zou Yi Zhang Qingrong Zhang Kesu Hu |
| author_sort | Zhihan Zhu |
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| description | Abstract Hepatocyte growth factor (HGF) is a substance that stimulates the proliferation of hepatocytes which promote healing. We developed a macrophage membrane-encapsulated nanosphere drug delivery system containing HGF for the study of burn wound healing. Twenty-seven Sprague–Dawley rats were randomly divided into three groups: a saline control (NS) group, an engineered macrophage membrane-encapsulated nanospheres (ETMM@NPS) group, and an engineered macrophage membrane-encapsulated nanospheres treatment with HGF-loaded gene (HGF@ETMM@NPS) group.The wound tissue sections were examined histologically using hematoxylin and eosin (H&E) and Masson trichrome staining. Immunohistochemistry and Western blotting were performed to determine the expression of relevant proteins. The wound-healing, blood flow and complete epithelialization rates were significantly better in the HGF@ETMM@NPS group compared to the NS and ETMM@NPS groups. Expression of B-cell lymphoma 2-associated X-protein was significantly lower, and B-cell lymphoma 2, cluster of differentiation 31, HGF, alpha smooth muscle actin, and PCNA expression was significantly higher in the HGF@ETMM@NPS group compared with the other two groups. PCNA and HGF expression was significantly up-regulated in the HGF@ETMM@NPS group. The HGF@ETMM@NPS complex drug delivery system used in this research promoted wound healing via effective delivery of HGF to burn wounds, thereby accelerating skin cell growth and migration. |
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| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
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| spelling | doaj-art-9415b67f95e04405a0f84eafb381ffef2025-01-26T12:24:54ZengNature PortfolioScientific Reports2045-23222025-01-0115111710.1038/s41598-025-86716-2Mechanism of engineered macrophage membrane bionic gene-carrying nanospheres for targeted drug delivery to promote wound repair in deep second-degree burnsZhihan Zhu0Xinghua Zhu1Shichen Miao2Bolin Wang3Zihan Li4Dinghao Zhang5Shentian Zou6Yi Zhang7Qingrong Zhang8Kesu Hu9Department of Burn and Plastic Surgery, Medical School of Nantong UniversityDepartment of Burn and Plastic Surgery, Affiliated Hospital of Nantong UniversityMedical School of Nantong UniversityDepartment of Burn and Plastic Surgery, Medical School of Nantong UniversityDepartment of Burn and Plastic Surgery, Medical School of Nantong UniversityMedical School of Nantong UniversityHainan Medical UniversityDepartment of Burn and Plastic Surgery, Affiliated Hospital of Nantong UniversityDepartment of Burn and Plastic Surgery, Affiliated Hospital of Nantong UniversityDepartment of Burn and Plastic Surgery, Affiliated Hospital of Nantong UniversityAbstract Hepatocyte growth factor (HGF) is a substance that stimulates the proliferation of hepatocytes which promote healing. We developed a macrophage membrane-encapsulated nanosphere drug delivery system containing HGF for the study of burn wound healing. Twenty-seven Sprague–Dawley rats were randomly divided into three groups: a saline control (NS) group, an engineered macrophage membrane-encapsulated nanospheres (ETMM@NPS) group, and an engineered macrophage membrane-encapsulated nanospheres treatment with HGF-loaded gene (HGF@ETMM@NPS) group.The wound tissue sections were examined histologically using hematoxylin and eosin (H&E) and Masson trichrome staining. Immunohistochemistry and Western blotting were performed to determine the expression of relevant proteins. The wound-healing, blood flow and complete epithelialization rates were significantly better in the HGF@ETMM@NPS group compared to the NS and ETMM@NPS groups. Expression of B-cell lymphoma 2-associated X-protein was significantly lower, and B-cell lymphoma 2, cluster of differentiation 31, HGF, alpha smooth muscle actin, and PCNA expression was significantly higher in the HGF@ETMM@NPS group compared with the other two groups. PCNA and HGF expression was significantly up-regulated in the HGF@ETMM@NPS group. The HGF@ETMM@NPS complex drug delivery system used in this research promoted wound healing via effective delivery of HGF to burn wounds, thereby accelerating skin cell growth and migration.https://doi.org/10.1038/s41598-025-86716-2Deep second-degree burnsTrabecular repairTargeted drug delivery systemPLGA nanospheresEngineered macrophage membranes |
| spellingShingle | Zhihan Zhu Xinghua Zhu Shichen Miao Bolin Wang Zihan Li Dinghao Zhang Shentian Zou Yi Zhang Qingrong Zhang Kesu Hu Mechanism of engineered macrophage membrane bionic gene-carrying nanospheres for targeted drug delivery to promote wound repair in deep second-degree burns Scientific Reports Deep second-degree burns Trabecular repair Targeted drug delivery system PLGA nanospheres Engineered macrophage membranes |
| title | Mechanism of engineered macrophage membrane bionic gene-carrying nanospheres for targeted drug delivery to promote wound repair in deep second-degree burns |
| title_full | Mechanism of engineered macrophage membrane bionic gene-carrying nanospheres for targeted drug delivery to promote wound repair in deep second-degree burns |
| title_fullStr | Mechanism of engineered macrophage membrane bionic gene-carrying nanospheres for targeted drug delivery to promote wound repair in deep second-degree burns |
| title_full_unstemmed | Mechanism of engineered macrophage membrane bionic gene-carrying nanospheres for targeted drug delivery to promote wound repair in deep second-degree burns |
| title_short | Mechanism of engineered macrophage membrane bionic gene-carrying nanospheres for targeted drug delivery to promote wound repair in deep second-degree burns |
| title_sort | mechanism of engineered macrophage membrane bionic gene carrying nanospheres for targeted drug delivery to promote wound repair in deep second degree burns |
| topic | Deep second-degree burns Trabecular repair Targeted drug delivery system PLGA nanospheres Engineered macrophage membranes |
| url | https://doi.org/10.1038/s41598-025-86716-2 |
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