Genomic analysis, evolution and characterization of the heat shock protein-70 gene family in foxtail millet [Setaria italica (L.) P.Beauv.] along with expression analysis under saline conditions
Under stress conditions, heat shock proteins (HSPs) serve as fundamental regulators of cellular homeostasis. The molecular chaperone HSP70 belongs to a group of stress-related proteins which play important roles across growth and developmental events. The production of HSP70 proteins significantly i...
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Society of Eminent Biological Scientists
2025-04-01
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| Series: | International Journal of Applied and Experimental Biology |
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| Online Access: | https://submissions.eminentscientists.com/index.php/IJAEB/article/view/156 |
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| author | Jawad Ali Ali Raza Muhammad Aakif Khan Aqib Zeb Maliha Ghaffar Maqsood Ahmed Khaskheli |
| author_facet | Jawad Ali Ali Raza Muhammad Aakif Khan Aqib Zeb Maliha Ghaffar Maqsood Ahmed Khaskheli |
| author_sort | Jawad Ali |
| collection | DOAJ |
| description | Under stress conditions, heat shock proteins (HSPs) serve as fundamental regulators of cellular homeostasis. The molecular chaperone HSP70 belongs to a group of stress-related proteins which play important roles across growth and developmental events. The production of HSP70 proteins significantly increases during stress events including heat exposure, and salinity and drought conditions to protect proteins and maintain crucial macromolecular structures, thereby improving overall plant stress tolerance. This study identified 28 HSP70 genes in Setaria italica through phylogenetic analysis and placed these genes into four separate clades. The preserved structural elements and functional domains and conserved sequence patterns indicated that HSP70 genes maintain evolutionary stability. The HSP70 gene family spanned nine chromosomal scaffolds and most protein products were predicted to function in the cytoplasm. The analysis of synonymous substitutions and non-synonymous substitutions showed that HSP70 genes underwent both purifying and positive selection processes leading to their functional divergence. This study outcomes deliver a significant understanding of HSP70 genes' functional roles together with their association with plant growth and developmental processes. |
| format | Article |
| id | doaj-art-93f6c08b1e4841ac8701803341da4a92 |
| institution | Kabale University |
| issn | 2790-6523 2790-6531 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Society of Eminent Biological Scientists |
| record_format | Article |
| series | International Journal of Applied and Experimental Biology |
| spelling | doaj-art-93f6c08b1e4841ac8701803341da4a922025-08-20T03:53:42ZengSociety of Eminent Biological ScientistsInternational Journal of Applied and Experimental Biology2790-65232790-65312025-04-0110.56612/ijaaeb.v1i1.156157Genomic analysis, evolution and characterization of the heat shock protein-70 gene family in foxtail millet [Setaria italica (L.) P.Beauv.] along with expression analysis under saline conditionsJawad Ali0Ali Raza1Muhammad Aakif Khan2Aqib Zeb3Maliha Ghaffar4Maqsood Ahmed Khaskheli5Department of Biochemistry, University of Okara, Okara, PakistanAnhui Province Key Laboratory of Microbial Pest Management, School of Plant Protection, Anhui Agricultural University, Hefei, 230036, Anhui, P.R. ChinaDepartment of Plant Breeding and Genetics, Faculty of Agriculture, University of Agriculture, Faisalabad, PakistanState Key Laboratory of Rice Biology, China National Centre for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, P.R. ChinaDepartment of Biology, Faculty of Life Sciences, University of Okara, Okara, PakistanDepartment of Plant Pathology, College of Agriculture, Guizhou University, Guiyang, 550001, P.R. ChinaUnder stress conditions, heat shock proteins (HSPs) serve as fundamental regulators of cellular homeostasis. The molecular chaperone HSP70 belongs to a group of stress-related proteins which play important roles across growth and developmental events. The production of HSP70 proteins significantly increases during stress events including heat exposure, and salinity and drought conditions to protect proteins and maintain crucial macromolecular structures, thereby improving overall plant stress tolerance. This study identified 28 HSP70 genes in Setaria italica through phylogenetic analysis and placed these genes into four separate clades. The preserved structural elements and functional domains and conserved sequence patterns indicated that HSP70 genes maintain evolutionary stability. The HSP70 gene family spanned nine chromosomal scaffolds and most protein products were predicted to function in the cytoplasm. The analysis of synonymous substitutions and non-synonymous substitutions showed that HSP70 genes underwent both purifying and positive selection processes leading to their functional divergence. This study outcomes deliver a significant understanding of HSP70 genes' functional roles together with their association with plant growth and developmental processes.https://submissions.eminentscientists.com/index.php/IJAEB/article/view/156gene structuregenome-wide analysisheat shock proteinshsp70 genestress tolerance |
| spellingShingle | Jawad Ali Ali Raza Muhammad Aakif Khan Aqib Zeb Maliha Ghaffar Maqsood Ahmed Khaskheli Genomic analysis, evolution and characterization of the heat shock protein-70 gene family in foxtail millet [Setaria italica (L.) P.Beauv.] along with expression analysis under saline conditions International Journal of Applied and Experimental Biology gene structure genome-wide analysis heat shock proteins hsp70 gene stress tolerance |
| title | Genomic analysis, evolution and characterization of the heat shock protein-70 gene family in foxtail millet [Setaria italica (L.) P.Beauv.] along with expression analysis under saline conditions |
| title_full | Genomic analysis, evolution and characterization of the heat shock protein-70 gene family in foxtail millet [Setaria italica (L.) P.Beauv.] along with expression analysis under saline conditions |
| title_fullStr | Genomic analysis, evolution and characterization of the heat shock protein-70 gene family in foxtail millet [Setaria italica (L.) P.Beauv.] along with expression analysis under saline conditions |
| title_full_unstemmed | Genomic analysis, evolution and characterization of the heat shock protein-70 gene family in foxtail millet [Setaria italica (L.) P.Beauv.] along with expression analysis under saline conditions |
| title_short | Genomic analysis, evolution and characterization of the heat shock protein-70 gene family in foxtail millet [Setaria italica (L.) P.Beauv.] along with expression analysis under saline conditions |
| title_sort | genomic analysis evolution and characterization of the heat shock protein 70 gene family in foxtail millet setaria italica l p beauv along with expression analysis under saline conditions |
| topic | gene structure genome-wide analysis heat shock proteins hsp70 gene stress tolerance |
| url | https://submissions.eminentscientists.com/index.php/IJAEB/article/view/156 |
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