Differential impact of glycoprotein glycosylation on Akkermansia muciniphila growth dynamics
Akkermansia muciniphila, a pivotal symbiotic bacterium resident in the human gut mucosal layer, significantly influences the intricate balance between health and disease states. This organism primarily thrives on mucin, a glycoprotein constituting the mucosal layer's main structural component....
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Maximum Academic Press
2024-01-01
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| Series: | Food Materials Research |
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| Online Access: | https://www.maxapress.com/article/doi/10.48130/fmr-0024-0013 |
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| author | Jia-Xin Zhang Yun-Sheng Lyu Josef Voglmeir Li Liu |
| author_facet | Jia-Xin Zhang Yun-Sheng Lyu Josef Voglmeir Li Liu |
| author_sort | Jia-Xin Zhang |
| collection | DOAJ |
| description | Akkermansia muciniphila, a pivotal symbiotic bacterium resident in the human gut mucosal layer, significantly influences the intricate balance between health and disease states. This organism primarily thrives on mucin, a glycoprotein constituting the mucosal layer's main structural component. Despite the recognized importance of mucin as a nutrient source for this bacterium, the specific contributions of its constituent protein and glycan chains to the probiotic effects of A. muciniphila remain unexplored. Herein, the individual and combined impacts of the protein backbone and O-linked glycans of mucin on bacterial growth are investigated. Traditional deglycosylation techniques, which tend to disrupt protein integrity, were bypassed by employing recombinant mucin variants, facilitating an accurate assessment. The present findings reveal that both protein and O-glycan components of mucin conspicuously foster A. muciniphila proliferation, with the O-glycans playing a paramount role. By assessing foodstuff glycoproteins as a nutritional source, a differential influence on A. muciniphila growth was observed, where glycan structures from milk and eggs exhibited a negligible or, in the case of milk, inhibitory effect. The study progresses to unravel the variations in A. muciniphila's response to different substrates through its exoglycosidase activity, alongside examining the growth modulation by distinct monosaccharide compositions and glycan chain structures. The present results show the complex interactions between A. muciniphila’s dietary interactions and underscore the necessity for in-depth studies focused on monosaccharide composition and glycan structural nuances to better comprehend their functional roles in A. muciniphila's probiotic activity. |
| format | Article |
| id | doaj-art-93e9f214f25d4a189bbd0e2b07c16350 |
| institution | DOAJ |
| issn | 2771-4683 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Maximum Academic Press |
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| series | Food Materials Research |
| spelling | doaj-art-93e9f214f25d4a189bbd0e2b07c163502025-08-20T03:18:54ZengMaximum Academic PressFood Materials Research2771-46832024-01-014111110.48130/fmr-0024-0013fmr-0024-0013Differential impact of glycoprotein glycosylation on Akkermansia muciniphila growth dynamicsJia-Xin Zhang0Yun-Sheng Lyu1Josef Voglmeir2Li Liu3Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaGlycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaGlycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaGlycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaAkkermansia muciniphila, a pivotal symbiotic bacterium resident in the human gut mucosal layer, significantly influences the intricate balance between health and disease states. This organism primarily thrives on mucin, a glycoprotein constituting the mucosal layer's main structural component. Despite the recognized importance of mucin as a nutrient source for this bacterium, the specific contributions of its constituent protein and glycan chains to the probiotic effects of A. muciniphila remain unexplored. Herein, the individual and combined impacts of the protein backbone and O-linked glycans of mucin on bacterial growth are investigated. Traditional deglycosylation techniques, which tend to disrupt protein integrity, were bypassed by employing recombinant mucin variants, facilitating an accurate assessment. The present findings reveal that both protein and O-glycan components of mucin conspicuously foster A. muciniphila proliferation, with the O-glycans playing a paramount role. By assessing foodstuff glycoproteins as a nutritional source, a differential influence on A. muciniphila growth was observed, where glycan structures from milk and eggs exhibited a negligible or, in the case of milk, inhibitory effect. The study progresses to unravel the variations in A. muciniphila's response to different substrates through its exoglycosidase activity, alongside examining the growth modulation by distinct monosaccharide compositions and glycan chain structures. The present results show the complex interactions between A. muciniphila’s dietary interactions and underscore the necessity for in-depth studies focused on monosaccharide composition and glycan structural nuances to better comprehend their functional roles in A. muciniphila's probiotic activity.https://www.maxapress.com/article/doi/10.48130/fmr-0024-0013akkermansia muciniphilamucin o-glycanfoodstuff glycoproteinsglycoprotein glycosylationprobiotic function. |
| spellingShingle | Jia-Xin Zhang Yun-Sheng Lyu Josef Voglmeir Li Liu Differential impact of glycoprotein glycosylation on Akkermansia muciniphila growth dynamics Food Materials Research akkermansia muciniphila mucin o-glycan foodstuff glycoproteins glycoprotein glycosylation probiotic function. |
| title | Differential impact of glycoprotein glycosylation on Akkermansia muciniphila growth dynamics |
| title_full | Differential impact of glycoprotein glycosylation on Akkermansia muciniphila growth dynamics |
| title_fullStr | Differential impact of glycoprotein glycosylation on Akkermansia muciniphila growth dynamics |
| title_full_unstemmed | Differential impact of glycoprotein glycosylation on Akkermansia muciniphila growth dynamics |
| title_short | Differential impact of glycoprotein glycosylation on Akkermansia muciniphila growth dynamics |
| title_sort | differential impact of glycoprotein glycosylation on akkermansia muciniphila growth dynamics |
| topic | akkermansia muciniphila mucin o-glycan foodstuff glycoproteins glycoprotein glycosylation probiotic function. |
| url | https://www.maxapress.com/article/doi/10.48130/fmr-0024-0013 |
| work_keys_str_mv | AT jiaxinzhang differentialimpactofglycoproteinglycosylationonakkermansiamuciniphilagrowthdynamics AT yunshenglyu differentialimpactofglycoproteinglycosylationonakkermansiamuciniphilagrowthdynamics AT josefvoglmeir differentialimpactofglycoproteinglycosylationonakkermansiamuciniphilagrowthdynamics AT liliu differentialimpactofglycoproteinglycosylationonakkermansiamuciniphilagrowthdynamics |