Targeted Urinary Profiling as a Biomarker of Dietary Flavonoid Intake from Fruits and Vegetables and its Relationship to the Timing of Dietary Assessment
Background: Dietary intake of flavonoids, a class of plant (poly)phenols, is hypothesized to mediate a variety of beneficial health outcomes linked to fruits and vegetables (FV) intake. Absorbed flavonoids are metabolized and excreted in urine and may serve as biomarkers of FV intake and host metabo...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-09-01
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| Series: | Current Developments in Nutrition |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2475299125029816 |
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| author | Emily B Hill Anna Pashkova Elizabeth M Grainger Kristen M Roberts Chureeporn Chitchumroonchokchai Ashlea C Braun Robin A Ralston Ken M Riedl Colleen K Spees Steven K Clinton |
| author_facet | Emily B Hill Anna Pashkova Elizabeth M Grainger Kristen M Roberts Chureeporn Chitchumroonchokchai Ashlea C Braun Robin A Ralston Ken M Riedl Colleen K Spees Steven K Clinton |
| author_sort | Emily B Hill |
| collection | DOAJ |
| description | Background: Dietary intake of flavonoids, a class of plant (poly)phenols, is hypothesized to mediate a variety of beneficial health outcomes linked to fruits and vegetables (FV) intake. Absorbed flavonoids are metabolized and excreted in urine and may serve as biomarkers of FV intake and host metabolism, but defining the relationship of urinary biomarkers to the specific time of intake is unclear. Objectives: To characterize a targeted panel of 6 urinary flavonoids and determine the relationship to estimated dietary flavonoid intake over time in adults. Methods: Dietary flavonoid intake was estimated from both a 30-d food frequency questionnaire and 3-d diet records using the Phenol-Explorer database in 17 adults. Twenty-four-hour urine was collected, and a targeted panel of 6 urinary flavonoids (quercetin, phloretin, naringenin, hesperetin, kaempferol, and isorhamnetin) was quantified using high-pressure liquid chromatography-diode array detection (DAD). Results: Compared to estimated intakes from a 1 d or 3 d diet record, total urinary flavonoids were most strongly associated with total FV (excluding potatoes) (rs = 0.53, P = 0.028) and total FV flavonoid (rs = 0.60, P = 0.011) intakes estimated by a 2 d diet record (including the day before and the day of the 24-h urine collection). There were no significant correlations between urinary concentrations of flavonoids and estimated FV intake using a 30-d food frequency questionnaire (rs = 0.36, P = 0.16). Conclusions: Targeted flavonoid concentrations in a 24-h urine collection may serve as a biomarker for estimating dietary exposures over 2 d prior to completion of the urine collection, but not more distant dietary exposures.This trial was registered at clinicaltrials.gov as NCT03489213. |
| format | Article |
| id | doaj-art-93e2f6d89b674d8682f7af0aa88d196e |
| institution | Kabale University |
| issn | 2475-2991 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Developments in Nutrition |
| spelling | doaj-art-93e2f6d89b674d8682f7af0aa88d196e2025-08-24T05:13:58ZengElsevierCurrent Developments in Nutrition2475-29912025-09-019910752010.1016/j.cdnut.2025.107520Targeted Urinary Profiling as a Biomarker of Dietary Flavonoid Intake from Fruits and Vegetables and its Relationship to the Timing of Dietary AssessmentEmily B Hill0Anna Pashkova1Elizabeth M Grainger2Kristen M Roberts3Chureeporn Chitchumroonchokchai4Ashlea C Braun5Robin A Ralston6Ken M Riedl7Colleen K Spees8Steven K Clinton9School of Health and Rehabilitation Sciences, College of Medicine, The Ohio State University, Columbus, OH, United States; Department of Pediatrics, Section of Nutrition, University of Colorado Anschutz Medical Campus, Aurora, CO, United StatesSchool of Health and Rehabilitation Sciences, College of Medicine, The Ohio State University, Columbus, OH, United StatesComprehensive Cancer Center, The Ohio State University, Columbus, OH, United States; Division of Medical Oncology, Department of Internal Medicine, The Ohio State University Wexner Medical Center, Columbus, OH, United States; Corresponding author.School of Health and Rehabilitation Sciences, College of Medicine, The Ohio State University, Columbus, OH, United StatesComprehensive Cancer Center, The Ohio State University, Columbus, OH, United States; Division of Medical Oncology, Department of Internal Medicine, The Ohio State University Wexner Medical Center, Columbus, OH, United StatesSchool of Health and Rehabilitation Sciences, College of Medicine, The Ohio State University, Columbus, OH, United States; Department of Health Promotion Sciences, Hudson College of Public Health, The University of Oklahoma Health Sciences, Oklahoma City, OK, United StatesComprehensive Cancer Center, The Ohio State University, Columbus, OH, United StatesComprehensive Cancer Center, The Ohio State University, Columbus, OH, United States; Department of Food Science and Technology, The Ohio State University, Columbus, OH, United StatesSchool of Health and Rehabilitation Sciences, College of Medicine, The Ohio State University, Columbus, OH, United States; Comprehensive Cancer Center, The Ohio State University, Columbus, OH, United StatesComprehensive Cancer Center, The Ohio State University, Columbus, OH, United States; Division of Medical Oncology, Department of Internal Medicine, The Ohio State University Wexner Medical Center, Columbus, OH, United StatesBackground: Dietary intake of flavonoids, a class of plant (poly)phenols, is hypothesized to mediate a variety of beneficial health outcomes linked to fruits and vegetables (FV) intake. Absorbed flavonoids are metabolized and excreted in urine and may serve as biomarkers of FV intake and host metabolism, but defining the relationship of urinary biomarkers to the specific time of intake is unclear. Objectives: To characterize a targeted panel of 6 urinary flavonoids and determine the relationship to estimated dietary flavonoid intake over time in adults. Methods: Dietary flavonoid intake was estimated from both a 30-d food frequency questionnaire and 3-d diet records using the Phenol-Explorer database in 17 adults. Twenty-four-hour urine was collected, and a targeted panel of 6 urinary flavonoids (quercetin, phloretin, naringenin, hesperetin, kaempferol, and isorhamnetin) was quantified using high-pressure liquid chromatography-diode array detection (DAD). Results: Compared to estimated intakes from a 1 d or 3 d diet record, total urinary flavonoids were most strongly associated with total FV (excluding potatoes) (rs = 0.53, P = 0.028) and total FV flavonoid (rs = 0.60, P = 0.011) intakes estimated by a 2 d diet record (including the day before and the day of the 24-h urine collection). There were no significant correlations between urinary concentrations of flavonoids and estimated FV intake using a 30-d food frequency questionnaire (rs = 0.36, P = 0.16). Conclusions: Targeted flavonoid concentrations in a 24-h urine collection may serve as a biomarker for estimating dietary exposures over 2 d prior to completion of the urine collection, but not more distant dietary exposures.This trial was registered at clinicaltrials.gov as NCT03489213.http://www.sciencedirect.com/science/article/pii/S2475299125029816dietary assessmentdietary biomarkersflavonoids(poly)phenolstargeted urinary metabolites |
| spellingShingle | Emily B Hill Anna Pashkova Elizabeth M Grainger Kristen M Roberts Chureeporn Chitchumroonchokchai Ashlea C Braun Robin A Ralston Ken M Riedl Colleen K Spees Steven K Clinton Targeted Urinary Profiling as a Biomarker of Dietary Flavonoid Intake from Fruits and Vegetables and its Relationship to the Timing of Dietary Assessment Current Developments in Nutrition dietary assessment dietary biomarkers flavonoids (poly)phenols targeted urinary metabolites |
| title | Targeted Urinary Profiling as a Biomarker of Dietary Flavonoid Intake from Fruits and Vegetables and its Relationship to the Timing of Dietary Assessment |
| title_full | Targeted Urinary Profiling as a Biomarker of Dietary Flavonoid Intake from Fruits and Vegetables and its Relationship to the Timing of Dietary Assessment |
| title_fullStr | Targeted Urinary Profiling as a Biomarker of Dietary Flavonoid Intake from Fruits and Vegetables and its Relationship to the Timing of Dietary Assessment |
| title_full_unstemmed | Targeted Urinary Profiling as a Biomarker of Dietary Flavonoid Intake from Fruits and Vegetables and its Relationship to the Timing of Dietary Assessment |
| title_short | Targeted Urinary Profiling as a Biomarker of Dietary Flavonoid Intake from Fruits and Vegetables and its Relationship to the Timing of Dietary Assessment |
| title_sort | targeted urinary profiling as a biomarker of dietary flavonoid intake from fruits and vegetables and its relationship to the timing of dietary assessment |
| topic | dietary assessment dietary biomarkers flavonoids (poly)phenols targeted urinary metabolites |
| url | http://www.sciencedirect.com/science/article/pii/S2475299125029816 |
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