Optimization of Ultrasound-Assisted Extraction of Morphine from Capsules of Papaver somniferum by Response Surface Methodology

In this study, amount of morphine from poppy capsules (Papaver somniferum) was investigated using ultrasonic assisted extraction (UAE). Response surface methodology was used to estimate effective experimental conditions on the content extraction of poppy capsules. For this purpose, solvent/solid rat...

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Main Authors: Ibrahim Bulduk, Bahdışen Gezer, Mustafa Cengiz
Format: Article
Language:English
Published: Wiley 2015-01-01
Series:International Journal of Analytical Chemistry
Online Access:http://dx.doi.org/10.1155/2015/796349
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author Ibrahim Bulduk
Bahdışen Gezer
Mustafa Cengiz
author_facet Ibrahim Bulduk
Bahdışen Gezer
Mustafa Cengiz
author_sort Ibrahim Bulduk
collection DOAJ
description In this study, amount of morphine from poppy capsules (Papaver somniferum) was investigated using ultrasonic assisted extraction (UAE). Response surface methodology was used to estimate effective experimental conditions on the content extraction of poppy capsules. For this purpose, solvent/solid ratio (10–20 mL/500 mg sample), pH (1–13), time (30–60 min), and temperature (30–50°C) were chosen as experimental variables. The affected response is extraction recovery values for morphine from poppy straw. For interpreting the relationship between experimental factors and response, a design table was established with combinations of three different concentrations levels of this compound in 29 trials. The second order quadratic model gave a satisfactory description of the experimental data. In our study, R-Squared (0.96), Adj-R-Squared (0.92), and Pred R-Squared (0.78) values for extraction yield display good accuracy of the derived model. The predicted optimal conditions for the highest morphine level (3.38 mg morphine/500 mg-sample) were found at 19.99 mL solvent/500 mg solid ratio, 59.94 min extraction time, 1.10 pH, and 42.36°C temperature. In the optimal extraction conditions, the experimental values are very close to the predicted values. Consequently, the response surface modeling can be achieved sufficiently to predict extraction yield from poppy straw by ultrasound assisted extraction.
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spelling doaj-art-93d8cb29c12c4302b8d0dd2ea3f1c3882025-08-20T02:10:11ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792015-01-01201510.1155/2015/796349796349Optimization of Ultrasound-Assisted Extraction of Morphine from Capsules of Papaver somniferum by Response Surface MethodologyIbrahim Bulduk0Bahdışen Gezer1Mustafa Cengiz2Uşak University, Department of Chemical Engineering, 64200 Uşak, TurkeyUşak University, Department of Chemical Engineering, 64200 Uşak, TurkeySüleyman Demirel University, Department of Chemistry, 32000 Isparta, TurkeyIn this study, amount of morphine from poppy capsules (Papaver somniferum) was investigated using ultrasonic assisted extraction (UAE). Response surface methodology was used to estimate effective experimental conditions on the content extraction of poppy capsules. For this purpose, solvent/solid ratio (10–20 mL/500 mg sample), pH (1–13), time (30–60 min), and temperature (30–50°C) were chosen as experimental variables. The affected response is extraction recovery values for morphine from poppy straw. For interpreting the relationship between experimental factors and response, a design table was established with combinations of three different concentrations levels of this compound in 29 trials. The second order quadratic model gave a satisfactory description of the experimental data. In our study, R-Squared (0.96), Adj-R-Squared (0.92), and Pred R-Squared (0.78) values for extraction yield display good accuracy of the derived model. The predicted optimal conditions for the highest morphine level (3.38 mg morphine/500 mg-sample) were found at 19.99 mL solvent/500 mg solid ratio, 59.94 min extraction time, 1.10 pH, and 42.36°C temperature. In the optimal extraction conditions, the experimental values are very close to the predicted values. Consequently, the response surface modeling can be achieved sufficiently to predict extraction yield from poppy straw by ultrasound assisted extraction.http://dx.doi.org/10.1155/2015/796349
spellingShingle Ibrahim Bulduk
Bahdışen Gezer
Mustafa Cengiz
Optimization of Ultrasound-Assisted Extraction of Morphine from Capsules of Papaver somniferum by Response Surface Methodology
International Journal of Analytical Chemistry
title Optimization of Ultrasound-Assisted Extraction of Morphine from Capsules of Papaver somniferum by Response Surface Methodology
title_full Optimization of Ultrasound-Assisted Extraction of Morphine from Capsules of Papaver somniferum by Response Surface Methodology
title_fullStr Optimization of Ultrasound-Assisted Extraction of Morphine from Capsules of Papaver somniferum by Response Surface Methodology
title_full_unstemmed Optimization of Ultrasound-Assisted Extraction of Morphine from Capsules of Papaver somniferum by Response Surface Methodology
title_short Optimization of Ultrasound-Assisted Extraction of Morphine from Capsules of Papaver somniferum by Response Surface Methodology
title_sort optimization of ultrasound assisted extraction of morphine from capsules of papaver somniferum by response surface methodology
url http://dx.doi.org/10.1155/2015/796349
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AT bahdısengezer optimizationofultrasoundassistedextractionofmorphinefromcapsulesofpapaversomniferumbyresponsesurfacemethodology
AT mustafacengiz optimizationofultrasoundassistedextractionofmorphinefromcapsulesofpapaversomniferumbyresponsesurfacemethodology