Experimental Infection and Detection of Necrotizing Hepatopancreatitis Bacterium in the American Lobster Homarus americanus
Necrotizing hepatopancreatitis bacterium (NHPB) is an obligated intracellular bacteria causing severe hepatopancreatic damages and mass mortalities in penaeid shrimp. The worldwide distribution of penaeid shrimp as alien species threatens the life cycle of other crustacean species. The aim of the ex...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/979381 |
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| author | Luz A. Avila-Villa Teresa Gollas-Galván Marcel Martínez-Porchas Fernando Mendoza-Cano Jorge Hernández-López |
| author_facet | Luz A. Avila-Villa Teresa Gollas-Galván Marcel Martínez-Porchas Fernando Mendoza-Cano Jorge Hernández-López |
| author_sort | Luz A. Avila-Villa |
| collection | DOAJ |
| description | Necrotizing hepatopancreatitis bacterium (NHPB) is an obligated intracellular bacteria causing severe hepatopancreatic damages and mass mortalities in penaeid shrimp. The worldwide distribution of penaeid shrimp as alien species threatens the life cycle of other crustacean species. The aim of the experiment was to evaluate the possibility of experimentally infecting the American lobster (Homarus americanus) with NHPB extracted from shrimp hepatopancreas. Homogenates from infected shrimp were fed by force to lobsters. Other group of lobsters was fed with homogenates of NHPB-free hepatopancreas. After the 15th day from initial inoculation, the presence of NHPB was detected by polymerase chain reaction in feces and hepatopancreas from lobsters inoculated with infected homogenates. Necrotized spots were observed in the surface of lobster hepatopancreas. In contrast, lobsters fed on NHPB-free homogenates resulted negative for NHPB. Evidence suggests the plasticity of NHPB which can infect crustacean from different species and inhabiting diverse latitudes. Considering the results, the American lobster could be a good candidate to maintain available NHPB in vivo. |
| format | Article |
| id | doaj-art-93ce7eb1e63e4dceb6fb46aa2db6204a |
| institution | OA Journals |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-93ce7eb1e63e4dceb6fb46aa2db6204a2025-08-20T02:10:10ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/979381979381Experimental Infection and Detection of Necrotizing Hepatopancreatitis Bacterium in the American Lobster Homarus americanusLuz A. Avila-Villa0Teresa Gollas-Galván1Marcel Martínez-Porchas2Fernando Mendoza-Cano3Jorge Hernández-López4Centro de Investigación en Alimentación y Desarrollo, A.C., P.O. Box 1735, 83304 Hermosillo, SON, MexicoCentro de Investigación en Alimentación y Desarrollo, A.C., P.O. Box 1735, 83304 Hermosillo, SON, MexicoCentro de Investigación en Alimentación y Desarrollo, A.C., P.O. Box 1735, 83304 Hermosillo, SON, MexicoAquaculture Department, Centro de Investigaciones Biológicas del Noroeste, Avenida Centenario No. 53, 83000 Hermosillo, SON, MexicoAquaculture Department, Centro de Investigaciones Biológicas del Noroeste, Avenida Centenario No. 53, 83000 Hermosillo, SON, MexicoNecrotizing hepatopancreatitis bacterium (NHPB) is an obligated intracellular bacteria causing severe hepatopancreatic damages and mass mortalities in penaeid shrimp. The worldwide distribution of penaeid shrimp as alien species threatens the life cycle of other crustacean species. The aim of the experiment was to evaluate the possibility of experimentally infecting the American lobster (Homarus americanus) with NHPB extracted from shrimp hepatopancreas. Homogenates from infected shrimp were fed by force to lobsters. Other group of lobsters was fed with homogenates of NHPB-free hepatopancreas. After the 15th day from initial inoculation, the presence of NHPB was detected by polymerase chain reaction in feces and hepatopancreas from lobsters inoculated with infected homogenates. Necrotized spots were observed in the surface of lobster hepatopancreas. In contrast, lobsters fed on NHPB-free homogenates resulted negative for NHPB. Evidence suggests the plasticity of NHPB which can infect crustacean from different species and inhabiting diverse latitudes. Considering the results, the American lobster could be a good candidate to maintain available NHPB in vivo.http://dx.doi.org/10.1100/2012/979381 |
| spellingShingle | Luz A. Avila-Villa Teresa Gollas-Galván Marcel Martínez-Porchas Fernando Mendoza-Cano Jorge Hernández-López Experimental Infection and Detection of Necrotizing Hepatopancreatitis Bacterium in the American Lobster Homarus americanus The Scientific World Journal |
| title | Experimental Infection and Detection of Necrotizing Hepatopancreatitis Bacterium in the American Lobster Homarus americanus |
| title_full | Experimental Infection and Detection of Necrotizing Hepatopancreatitis Bacterium in the American Lobster Homarus americanus |
| title_fullStr | Experimental Infection and Detection of Necrotizing Hepatopancreatitis Bacterium in the American Lobster Homarus americanus |
| title_full_unstemmed | Experimental Infection and Detection of Necrotizing Hepatopancreatitis Bacterium in the American Lobster Homarus americanus |
| title_short | Experimental Infection and Detection of Necrotizing Hepatopancreatitis Bacterium in the American Lobster Homarus americanus |
| title_sort | experimental infection and detection of necrotizing hepatopancreatitis bacterium in the american lobster homarus americanus |
| url | http://dx.doi.org/10.1100/2012/979381 |
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