Seasonal changes in patterns of gene expression in avian song control brain regions.
Photoperiod and hormonal cues drive dramatic seasonal changes in structure and function of the avian song control system. Little is known, however, about the patterns of gene expression associated with seasonal changes. Here we address this issue by altering the hormonal and photoperiodic conditions...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0035119&type=printable |
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| _version_ | 1850138080165494784 |
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| author | Christopher K Thompson John Meitzen Kirstin Replogle Jenny Drnevich Karin L Lent Anne Marie Wissman Federico M Farin Theo K Bammler Richard P Beyer David F Clayton David J Perkel Eliot A Brenowitz |
| author_facet | Christopher K Thompson John Meitzen Kirstin Replogle Jenny Drnevich Karin L Lent Anne Marie Wissman Federico M Farin Theo K Bammler Richard P Beyer David F Clayton David J Perkel Eliot A Brenowitz |
| author_sort | Christopher K Thompson |
| collection | DOAJ |
| description | Photoperiod and hormonal cues drive dramatic seasonal changes in structure and function of the avian song control system. Little is known, however, about the patterns of gene expression associated with seasonal changes. Here we address this issue by altering the hormonal and photoperiodic conditions in seasonally-breeding Gambel's white-crowned sparrows and extracting RNA from the telencephalic song control nuclei HVC and RA across multiple time points that capture different stages of growth and regression. We chose HVC and RA because while both nuclei change in volume across seasons, the cellular mechanisms underlying these changes differ. We thus hypothesized that different genes would be expressed between HVC and RA. We tested this by using the extracted RNA to perform a cDNA microarray hybridization developed by the SoNG initiative. We then validated these results using qRT-PCR. We found that 363 genes varied by more than 1.5 fold (>log(2) 0.585) in expression in HVC and/or RA. Supporting our hypothesis, only 59 of these 363 genes were found to vary in both nuclei, while 132 gene expression changes were HVC specific and 172 were RA specific. We then assigned many of these genes to functional categories relevant to the different mechanisms underlying seasonal change in HVC and RA, including neurogenesis, apoptosis, cell growth, dendrite arborization and axonal growth, angiogenesis, endocrinology, growth factors, and electrophysiology. This revealed categorical differences in the kinds of genes regulated in HVC and RA. These results show that different molecular programs underlie seasonal changes in HVC and RA, and that gene expression is time specific across different reproductive conditions. Our results provide insights into the complex molecular pathways that underlie adult neural plasticity. |
| format | Article |
| id | doaj-art-93c187de22fa42ccb8ff054057788bb2 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-93c187de22fa42ccb8ff054057788bb22025-08-20T02:30:39ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0174e3511910.1371/journal.pone.0035119Seasonal changes in patterns of gene expression in avian song control brain regions.Christopher K ThompsonJohn MeitzenKirstin ReplogleJenny DrnevichKarin L LentAnne Marie WissmanFederico M FarinTheo K BammlerRichard P BeyerDavid F ClaytonDavid J PerkelEliot A BrenowitzPhotoperiod and hormonal cues drive dramatic seasonal changes in structure and function of the avian song control system. Little is known, however, about the patterns of gene expression associated with seasonal changes. Here we address this issue by altering the hormonal and photoperiodic conditions in seasonally-breeding Gambel's white-crowned sparrows and extracting RNA from the telencephalic song control nuclei HVC and RA across multiple time points that capture different stages of growth and regression. We chose HVC and RA because while both nuclei change in volume across seasons, the cellular mechanisms underlying these changes differ. We thus hypothesized that different genes would be expressed between HVC and RA. We tested this by using the extracted RNA to perform a cDNA microarray hybridization developed by the SoNG initiative. We then validated these results using qRT-PCR. We found that 363 genes varied by more than 1.5 fold (>log(2) 0.585) in expression in HVC and/or RA. Supporting our hypothesis, only 59 of these 363 genes were found to vary in both nuclei, while 132 gene expression changes were HVC specific and 172 were RA specific. We then assigned many of these genes to functional categories relevant to the different mechanisms underlying seasonal change in HVC and RA, including neurogenesis, apoptosis, cell growth, dendrite arborization and axonal growth, angiogenesis, endocrinology, growth factors, and electrophysiology. This revealed categorical differences in the kinds of genes regulated in HVC and RA. These results show that different molecular programs underlie seasonal changes in HVC and RA, and that gene expression is time specific across different reproductive conditions. Our results provide insights into the complex molecular pathways that underlie adult neural plasticity.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0035119&type=printable |
| spellingShingle | Christopher K Thompson John Meitzen Kirstin Replogle Jenny Drnevich Karin L Lent Anne Marie Wissman Federico M Farin Theo K Bammler Richard P Beyer David F Clayton David J Perkel Eliot A Brenowitz Seasonal changes in patterns of gene expression in avian song control brain regions. PLoS ONE |
| title | Seasonal changes in patterns of gene expression in avian song control brain regions. |
| title_full | Seasonal changes in patterns of gene expression in avian song control brain regions. |
| title_fullStr | Seasonal changes in patterns of gene expression in avian song control brain regions. |
| title_full_unstemmed | Seasonal changes in patterns of gene expression in avian song control brain regions. |
| title_short | Seasonal changes in patterns of gene expression in avian song control brain regions. |
| title_sort | seasonal changes in patterns of gene expression in avian song control brain regions |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0035119&type=printable |
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