Development of an automated plaque-counting program for the quantification of the Chikungunya virus

Development of an automated plaque-counting program for the quantification of the Chikungunya virus

Abstract Chikungunya virus (CHIKV) induces a massive cytopathic effect (CPE) on various cell types. Therefore, the plaque assay, a CPE-based virus titration method, remains the gold standard for quantifying the infectious units of CHIKV. However, manual plaque counting is often a labor-intensive tas...

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Bibliographic Details
Main Authors: Akino Emi, Youichi Suzuki, Chinami Odake, Shoichi Sakaguchi, Hong Wu, Takashi Nakano
Format: Article
Language:English
Published: Nature Portfolio 2025-04-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-025-97590-3
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