Characterization of a novel cell line established from mice gastrointestinal stromal model by chemical induction
Background: Gastrointestinal stromal tumors (GISTs) are a type of tumor that originates from gastrointestinal mesenchymal tissue. Although several somatic or germline mutation GIST mice were established, however, there is still a lack of an authentic mice GIST cell lines for further experimental stu...
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Elsevier
2025-06-01
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| Series: | Translational Oncology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1936523325001196 |
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| author | Zhan Zhao Shenghui Qiu Xiangwei Zhang Shijin Liu Lu Wang Hanyang Guan Jiashuai He Yangzhi Hu Xiaobo Li Simin Luo Zuyang Chen Tianmu Mo Yiran Zhang Xiaoxu Zhao Yunlong Pan Hui Ding Jie Cao Jinghua Pan |
| author_facet | Zhan Zhao Shenghui Qiu Xiangwei Zhang Shijin Liu Lu Wang Hanyang Guan Jiashuai He Yangzhi Hu Xiaobo Li Simin Luo Zuyang Chen Tianmu Mo Yiran Zhang Xiaoxu Zhao Yunlong Pan Hui Ding Jie Cao Jinghua Pan |
| author_sort | Zhan Zhao |
| collection | DOAJ |
| description | Background: Gastrointestinal stromal tumors (GISTs) are a type of tumor that originates from gastrointestinal mesenchymal tissue. Although several somatic or germline mutation GIST mice were established, however, there is still a lack of an authentic mice GIST cell lines for further experimental study. Methods: We developed a chemically induced C57BL/6 J GIST model using 3- methylcholanthrene. Tumor characteristics were confirmed through histology and IHC. Primary cells were isolated to establish the mGSTc01 cell line, and molecular profiling was conducted. Additionally, we established GIST model in immunocompetent mice to evaluate their sensitivity to imatinib. Results: Our study successfully developed a chemically induced murine GIST model, characterized by positive staining of c-kit and DOG-1. The mGSTc01 monoclonal cell line exhibited slender morphology and expressed the c-kit marker, Whole exome sequencing uncovered mutations of Lamb1, MMP9, and c-kit in GIST cells and provided a detailed picture of the entire genome's copy number variations. RNA sequencing indicated genes associated with cell adhesion and focal adhesion were enriched in mGSTc01 cells. The mGSTc01 cells demonstrated obvious malignant behaviors, notably elevated migration, adhesion, and proliferation. In immunocompetent mice, subcutaneous xenografts not only reserved the aggressive phenotype but also displayed a response to imatinib, underscoring the model's applicability for advancing therapeutic research. Conclusion: We firstly established a mGSTc01 cell line derived from C57BL/6 J mice GIST tumor offers, which closely mimicking human disease characteristics. It is a potent platform for investigating tumor microenvironment of GIST in mice model, and provides a novel way for new therapeutic discoveries in GIST. |
| format | Article |
| id | doaj-art-93acbe3d05ed4712aadfc3df1651caa7 |
| institution | DOAJ |
| issn | 1936-5233 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Translational Oncology |
| spelling | doaj-art-93acbe3d05ed4712aadfc3df1651caa72025-08-20T03:12:02ZengElsevierTranslational Oncology1936-52332025-06-015610238810.1016/j.tranon.2025.102388Characterization of a novel cell line established from mice gastrointestinal stromal model by chemical inductionZhan Zhao0Shenghui Qiu1Xiangwei Zhang2Shijin Liu3Lu Wang4Hanyang Guan5Jiashuai He6Yangzhi Hu7Xiaobo Li8Simin Luo9Zuyang Chen10Tianmu Mo11Yiran Zhang12Xiaoxu Zhao13Yunlong Pan14Hui Ding15Jie Cao16Jinghua Pan17Department of General Surgery, The First Affiliated Hospital of Jinan University, 510632, Guangzhou, Guangdong, PR ChinaDepartment of General Surgery, The First Affiliated Hospital of Jinan University, 510632, Guangzhou, Guangdong, PR China; Department of General Surgery, Guangzhou First People's Hospital, Guangzhou, 510180, PR ChinaDepartment of General Surgery, The First Affiliated Hospital of Jinan University, 510632, Guangzhou, Guangdong, PR ChinaDepartment of General Surgery, The First Affiliated Hospital of Jinan University, 510632, Guangzhou, Guangdong, PR ChinaInstitute of Precision Cancer Medicine and Pathology, Jinan University Medical College, Guangzhou, Guangdong, 510632, PR ChinaDepartment of General Surgery, The First Affiliated Hospital of Jinan University, 510632, Guangzhou, Guangdong, PR ChinaDepartment of General Surgery, The First Affiliated Hospital of Jinan University, 510632, Guangzhou, Guangdong, PR ChinaThe Affiliated Hospital of Xiangnan University, Chenzhou, Hunan, PR ChinaState Key Laboratory of Bioactive Molecules and Druggability Assessment, Jinan University, Guangzhou, 510632, ChinaDepartment of Bone and Joint Surgery, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong Province, ChinaDepartment of General Surgery, The First Affiliated Hospital of Jinan University, 510632, Guangzhou, Guangdong, PR ChinaDepartment of General Surgery, The First Affiliated Hospital of Jinan University, 510632, Guangzhou, Guangdong, PR ChinaDepartment of General Surgery, The First Affiliated Hospital of Jinan University, 510632, Guangzhou, Guangdong, PR ChinaDepartment of General Surgery, The First Affiliated Hospital of Jinan University, 510632, Guangzhou, Guangdong, PR ChinaDepartment of General Surgery, The First Affiliated Hospital of Jinan University, 510632, Guangzhou, Guangdong, PR ChinaDepartment of General Surgery, The First Affiliated Hospital of Jinan University, 510632, Guangzhou, Guangdong, PR China; Corresponding authors at: Department of General Surgery, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong, 510632, PR China.Department of General Surgery, The First Affiliated Hospital of Jinan University, 510632, Guangzhou, Guangdong, PR China; Department of General Surgery, Guangzhou First People's Hospital, Guangzhou, 510180, PR China; Corresponding authors at: Department of General Surgery, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong, 510632, PR China.Department of General Surgery, The First Affiliated Hospital of Jinan University, 510632, Guangzhou, Guangdong, PR China; Corresponding authors at: Department of General Surgery, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong, 510632, PR China.Background: Gastrointestinal stromal tumors (GISTs) are a type of tumor that originates from gastrointestinal mesenchymal tissue. Although several somatic or germline mutation GIST mice were established, however, there is still a lack of an authentic mice GIST cell lines for further experimental study. Methods: We developed a chemically induced C57BL/6 J GIST model using 3- methylcholanthrene. Tumor characteristics were confirmed through histology and IHC. Primary cells were isolated to establish the mGSTc01 cell line, and molecular profiling was conducted. Additionally, we established GIST model in immunocompetent mice to evaluate their sensitivity to imatinib. Results: Our study successfully developed a chemically induced murine GIST model, characterized by positive staining of c-kit and DOG-1. The mGSTc01 monoclonal cell line exhibited slender morphology and expressed the c-kit marker, Whole exome sequencing uncovered mutations of Lamb1, MMP9, and c-kit in GIST cells and provided a detailed picture of the entire genome's copy number variations. RNA sequencing indicated genes associated with cell adhesion and focal adhesion were enriched in mGSTc01 cells. The mGSTc01 cells demonstrated obvious malignant behaviors, notably elevated migration, adhesion, and proliferation. In immunocompetent mice, subcutaneous xenografts not only reserved the aggressive phenotype but also displayed a response to imatinib, underscoring the model's applicability for advancing therapeutic research. Conclusion: We firstly established a mGSTc01 cell line derived from C57BL/6 J mice GIST tumor offers, which closely mimicking human disease characteristics. It is a potent platform for investigating tumor microenvironment of GIST in mice model, and provides a novel way for new therapeutic discoveries in GIST.http://www.sciencedirect.com/science/article/pii/S1936523325001196Gastrointestinal stromal tumors (GIST)mGSTc01Mouse cell lineC57BL/6 JC-kit |
| spellingShingle | Zhan Zhao Shenghui Qiu Xiangwei Zhang Shijin Liu Lu Wang Hanyang Guan Jiashuai He Yangzhi Hu Xiaobo Li Simin Luo Zuyang Chen Tianmu Mo Yiran Zhang Xiaoxu Zhao Yunlong Pan Hui Ding Jie Cao Jinghua Pan Characterization of a novel cell line established from mice gastrointestinal stromal model by chemical induction Translational Oncology Gastrointestinal stromal tumors (GIST) mGSTc01 Mouse cell line C57BL/6 J C-kit |
| title | Characterization of a novel cell line established from mice gastrointestinal stromal model by chemical induction |
| title_full | Characterization of a novel cell line established from mice gastrointestinal stromal model by chemical induction |
| title_fullStr | Characterization of a novel cell line established from mice gastrointestinal stromal model by chemical induction |
| title_full_unstemmed | Characterization of a novel cell line established from mice gastrointestinal stromal model by chemical induction |
| title_short | Characterization of a novel cell line established from mice gastrointestinal stromal model by chemical induction |
| title_sort | characterization of a novel cell line established from mice gastrointestinal stromal model by chemical induction |
| topic | Gastrointestinal stromal tumors (GIST) mGSTc01 Mouse cell line C57BL/6 J C-kit |
| url | http://www.sciencedirect.com/science/article/pii/S1936523325001196 |
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