Efecto de tiempo y temperatura de cocción en chorizo inoculados artificialmente con Listeria monocytogenes

Effect of cooking time and temperature on sausages artificially inoculated with Listeria monocytogenes. Objective. To find whetherthe cooking times traditionally used by consumers are enough to inactivate Listeria monocytogenes artificially inoculated into sausages.Materials and methods. A survey as...

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Main Authors: Ana K. Carrascal-Camacho, Marcela Mercado-Reyes, Silvia Nataly Molina-Moreno
Format: Article
Language:English
Published: Pontificia Universidad Javeriana 2009-12-01
Series:Universitas Scientiarum
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Online Access:http://revistas.javeriana.edu.co/index.php/scientarium/article/view/1390/852
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author Ana K. Carrascal-Camacho
Marcela Mercado-Reyes
Silvia Nataly Molina-Moreno
author_facet Ana K. Carrascal-Camacho
Marcela Mercado-Reyes
Silvia Nataly Molina-Moreno
author_sort Ana K. Carrascal-Camacho
collection DOAJ
description Effect of cooking time and temperature on sausages artificially inoculated with Listeria monocytogenes. Objective. To find whetherthe cooking times traditionally used by consumers are enough to inactivate Listeria monocytogenes artificially inoculated into sausages.Materials and methods. A survey asking about sausage cooking habits was completed with 50 housewives. We analyzed 60 samples ofsausages previously inoculated with 103 CFU g-1 from a pool of 5 strains of L. monocytogenes, then the cooking procedures described inthe survey were applied to the samples, and counting was done immediately after. Additionally, a complementary test was carried out byinoculating 20 samples of sausages with 103 CFU g-1 and exposing them to 72 oC and 73 oC for 30 seg. Results. The survey showed that15 minute boiling and 5 minute frying are the most frequent ways of preparing sausages by consumers. We established that the time andcooking conditions used in our assay had a statistically significant effect (p: 0.016) on the inoculated samples. In the complementary assay,statistical data (p: 0.0001) indicated that internal temperatures of 73 oC are enough to inactivate the pathogen at an industrial scale.Conclusion. Cooking by 15 minute boiling and 5 minute frying are enough to inactive concentrations of 103 g-1 of Listeria monocytogenesartificially inoculated into sausages.
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publisher Pontificia Universidad Javeriana
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spelling doaj-art-93a435aa51c24d72bebc0ca76aaaf1062025-08-20T02:10:10ZengPontificia Universidad JaverianaUniversitas Scientiarum0122-74832027-13522009-12-01142-3198205Efecto de tiempo y temperatura de cocción en chorizo inoculados artificialmente con Listeria monocytogenesAna K. Carrascal-CamachoMarcela Mercado-ReyesSilvia Nataly Molina-MorenoEffect of cooking time and temperature on sausages artificially inoculated with Listeria monocytogenes. Objective. To find whetherthe cooking times traditionally used by consumers are enough to inactivate Listeria monocytogenes artificially inoculated into sausages.Materials and methods. A survey asking about sausage cooking habits was completed with 50 housewives. We analyzed 60 samples ofsausages previously inoculated with 103 CFU g-1 from a pool of 5 strains of L. monocytogenes, then the cooking procedures described inthe survey were applied to the samples, and counting was done immediately after. Additionally, a complementary test was carried out byinoculating 20 samples of sausages with 103 CFU g-1 and exposing them to 72 oC and 73 oC for 30 seg. Results. The survey showed that15 minute boiling and 5 minute frying are the most frequent ways of preparing sausages by consumers. We established that the time andcooking conditions used in our assay had a statistically significant effect (p: 0.016) on the inoculated samples. In the complementary assay,statistical data (p: 0.0001) indicated that internal temperatures of 73 oC are enough to inactivate the pathogen at an industrial scale.Conclusion. Cooking by 15 minute boiling and 5 minute frying are enough to inactive concentrations of 103 g-1 of Listeria monocytogenesartificially inoculated into sausages.http://revistas.javeriana.edu.co/index.php/scientarium/article/view/1390/852listeriosisraw sausagesthermoresistance
spellingShingle Ana K. Carrascal-Camacho
Marcela Mercado-Reyes
Silvia Nataly Molina-Moreno
Efecto de tiempo y temperatura de cocción en chorizo inoculados artificialmente con Listeria monocytogenes
Universitas Scientiarum
listeriosis
raw sausages
thermoresistance
title Efecto de tiempo y temperatura de cocción en chorizo inoculados artificialmente con Listeria monocytogenes
title_full Efecto de tiempo y temperatura de cocción en chorizo inoculados artificialmente con Listeria monocytogenes
title_fullStr Efecto de tiempo y temperatura de cocción en chorizo inoculados artificialmente con Listeria monocytogenes
title_full_unstemmed Efecto de tiempo y temperatura de cocción en chorizo inoculados artificialmente con Listeria monocytogenes
title_short Efecto de tiempo y temperatura de cocción en chorizo inoculados artificialmente con Listeria monocytogenes
title_sort efecto de tiempo y temperatura de coccion en chorizo inoculados artificialmente con listeria monocytogenes
topic listeriosis
raw sausages
thermoresistance
url http://revistas.javeriana.edu.co/index.php/scientarium/article/view/1390/852
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AT marcelamercadoreyes efectodetiempoytemperaturadecoccionenchorizoinoculadosartificialmenteconlisteriamonocytogenes
AT silvianatalymolinamoreno efectodetiempoytemperaturadecoccionenchorizoinoculadosartificialmenteconlisteriamonocytogenes