Triclosan induces pyroptosis by activation of the caspase-9/3/gasdermin E axis
The high concentrations of TCS in personal care products, and the potential for even greater exposure in occupational settings, raise significant concerns about its cytotoxic effects. Numorous studies highlight the importance of understanding the molecular mechanisms of pyroptosis in toxicological r...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2025-03-01
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| Series: | Emerging Contaminants |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405665024001264 |
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| author | Shiqi Wu Lei You Shan He Wenqaing Liu Jinlin Lei Jiahui Yang Xiangyin Luo Zhenxiu Ye Yonghong Zhang Jing Wang Huailan Guo Yan Zheng Lanlan Zheng Chen Li |
| author_facet | Shiqi Wu Lei You Shan He Wenqaing Liu Jinlin Lei Jiahui Yang Xiangyin Luo Zhenxiu Ye Yonghong Zhang Jing Wang Huailan Guo Yan Zheng Lanlan Zheng Chen Li |
| author_sort | Shiqi Wu |
| collection | DOAJ |
| description | The high concentrations of TCS in personal care products, and the potential for even greater exposure in occupational settings, raise significant concerns about its cytotoxic effects. Numorous studies highlight the importance of understanding the molecular mechanisms of pyroptosis in toxicological research on environmental pollutants. However, it remains unclear whether TCS exposure could induce GSDME-mediated pyroptosis. In this study, we aimed to investigate the cytotoxic effects of 200 μM TCS on L02 cells and elucidate the molecular mechanisms involved in TCS-induced pyroptosis, a novel form of cell death. Our results demonstrate that TCS inhibits the proliferation of L02 cells in a dose-dependent manner and triggers caspase-dependent cell death, leading to mitochondrial dysfunction and subsequent pyroptosis through the activation of the caspase-9/3/GSDME axis. Furthermore, through transcriptional and metabolomic analyses, we identified alterations in the PI3K-Akt and MAPK cellular signaling pathways, as well as changes in carbon and nitrogen metabolism. Our data provide valuable insights into the biotoxicity of high TCS concentrations and establish a theoretical basis for future studies on its impact and risk. |
| format | Article |
| id | doaj-art-939f8dfeaf354f8c808c985f4e55c4f5 |
| institution | OA Journals |
| issn | 2405-6650 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Emerging Contaminants |
| spelling | doaj-art-939f8dfeaf354f8c808c985f4e55c4f52025-08-20T02:27:47ZengKeAi Communications Co., Ltd.Emerging Contaminants2405-66502025-03-0111110042510.1016/j.emcon.2024.100425Triclosan induces pyroptosis by activation of the caspase-9/3/gasdermin E axisShiqi Wu0Lei You1Shan He2Wenqaing Liu3Jinlin Lei4Jiahui Yang5Xiangyin Luo6Zhenxiu Ye7Yonghong Zhang8Jing Wang9Huailan Guo10Yan Zheng11Lanlan Zheng12Chen Li13Laboratory of Medicinal Plant, Hubei Key Laboratory of Embryonic Stem Cell Research, School of Basic Medicine, Hubei University of Medicine, Shiyan, 442000, ChinaLaboratory of Medicinal Plant, Hubei Key Laboratory of Embryonic Stem Cell Research, School of Basic Medicine, Hubei University of Medicine, Shiyan, 442000, ChinaLaboratory of Medicinal Plant, Hubei Key Laboratory of Embryonic Stem Cell Research, School of Basic Medicine, Hubei University of Medicine, Shiyan, 442000, ChinaLaboratory of Medicinal Plant, Hubei Key Laboratory of Embryonic Stem Cell Research, School of Basic Medicine, Hubei University of Medicine, Shiyan, 442000, ChinaResearch Center of Environment and Healh of South-to-North Waler Diversion Area, Hubei University of Medicine, Shiyan, 442000, ChinaLaboratory of Medicinal Plant, Hubei Key Laboratory of Embryonic Stem Cell Research, School of Basic Medicine, Hubei University of Medicine, Shiyan, 442000, ChinaLaboratory of Medicinal Plant, Hubei Key Laboratory of Embryonic Stem Cell Research, School of Basic Medicine, Hubei University of Medicine, Shiyan, 442000, ChinaLaboratory of Medicinal Plant, Hubei Key Laboratory of Embryonic Stem Cell Research, School of Basic Medicine, Hubei University of Medicine, Shiyan, 442000, ChinaLaboratory of Medicinal Plant, Hubei Key Laboratory of Embryonic Stem Cell Research, School of Basic Medicine, Hubei University of Medicine, Shiyan, 442000, ChinaResearch Center of Environment and Healh of South-to-North Waler Diversion Area, Hubei University of Medicine, Shiyan, 442000, ChinaResearch Center of Environment and Healh of South-to-North Waler Diversion Area, Hubei University of Medicine, Shiyan, 442000, ChinaDepartment of Pharmacy, Hubei Aerospace Hospital, Xiaogan, Hubei, 432000, ChinaLaboratory of Medicinal Plant, Hubei Key Laboratory of Embryonic Stem Cell Research, School of Basic Medicine, Hubei University of Medicine, Shiyan, 442000, ChinaLaboratory of Medicinal Plant, Hubei Key Laboratory of Embryonic Stem Cell Research, School of Basic Medicine, Hubei University of Medicine, Shiyan, 442000, China; Corresponding author.The high concentrations of TCS in personal care products, and the potential for even greater exposure in occupational settings, raise significant concerns about its cytotoxic effects. Numorous studies highlight the importance of understanding the molecular mechanisms of pyroptosis in toxicological research on environmental pollutants. However, it remains unclear whether TCS exposure could induce GSDME-mediated pyroptosis. In this study, we aimed to investigate the cytotoxic effects of 200 μM TCS on L02 cells and elucidate the molecular mechanisms involved in TCS-induced pyroptosis, a novel form of cell death. Our results demonstrate that TCS inhibits the proliferation of L02 cells in a dose-dependent manner and triggers caspase-dependent cell death, leading to mitochondrial dysfunction and subsequent pyroptosis through the activation of the caspase-9/3/GSDME axis. Furthermore, through transcriptional and metabolomic analyses, we identified alterations in the PI3K-Akt and MAPK cellular signaling pathways, as well as changes in carbon and nitrogen metabolism. Our data provide valuable insights into the biotoxicity of high TCS concentrations and establish a theoretical basis for future studies on its impact and risk.http://www.sciencedirect.com/science/article/pii/S2405665024001264Environmental toxicologyCytotoxicityTriclosanCell death |
| spellingShingle | Shiqi Wu Lei You Shan He Wenqaing Liu Jinlin Lei Jiahui Yang Xiangyin Luo Zhenxiu Ye Yonghong Zhang Jing Wang Huailan Guo Yan Zheng Lanlan Zheng Chen Li Triclosan induces pyroptosis by activation of the caspase-9/3/gasdermin E axis Emerging Contaminants Environmental toxicology Cytotoxicity Triclosan Cell death |
| title | Triclosan induces pyroptosis by activation of the caspase-9/3/gasdermin E axis |
| title_full | Triclosan induces pyroptosis by activation of the caspase-9/3/gasdermin E axis |
| title_fullStr | Triclosan induces pyroptosis by activation of the caspase-9/3/gasdermin E axis |
| title_full_unstemmed | Triclosan induces pyroptosis by activation of the caspase-9/3/gasdermin E axis |
| title_short | Triclosan induces pyroptosis by activation of the caspase-9/3/gasdermin E axis |
| title_sort | triclosan induces pyroptosis by activation of the caspase 9 3 gasdermin e axis |
| topic | Environmental toxicology Cytotoxicity Triclosan Cell death |
| url | http://www.sciencedirect.com/science/article/pii/S2405665024001264 |
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