Serovar and multilocus sequence typing analysis of Pasteurella multocida from diseased pigs in Taiwan
Abstract Background Pasteurella multocida causes progressive atrophic rhinitis and suppurative bronchopneumonia in pigs, which results in severe economic losses in swine industry. This study aimed to determine the serovar, genotype and prevalence of toxA virulence gene of Pasteurella multocida isola...
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BMC
2025-02-01
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| Series: | BMC Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s12917-025-04595-1 |
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| author | Ching-Fen Wu Che-Cheng Liao Chi-Chung Chou Chao-Min Wang Szu-Wei Huang Hung-Chih Kuo |
| author_facet | Ching-Fen Wu Che-Cheng Liao Chi-Chung Chou Chao-Min Wang Szu-Wei Huang Hung-Chih Kuo |
| author_sort | Ching-Fen Wu |
| collection | DOAJ |
| description | Abstract Background Pasteurella multocida causes progressive atrophic rhinitis and suppurative bronchopneumonia in pigs, which results in severe economic losses in swine industry. This study aimed to determine the serovar, genotype and prevalence of toxA virulence gene of Pasteurella multocida isolates collected in Taiwan. A total of 164 Pasteurella multocida isolates from 161 diseased pigs were characterized by serotyping, multilocus sequence typing (MLST), antimicrobial susceptibility testing, and the presence of virulence gene (toxA) and antibiotic resistance gene (floR). Results The majority of Pasteurella multocida strains were serovar D:L6 (48.2%; 79/164) followed by A:L6 (28.7%; 47/164) and A:L3 (19.5%; 32/164). More than 80% of strains carrying toxA gene belonged to serovar A:L6 (82.6%; 19/23). The MLST data showed five sequence types (STs), where multi-host ST10 was the most dominant. Most Pasteurella multocida strains of multi-host ST10 were serovar A:L6 (93.9%; 31/33), which suggested that STs were highly associated with specific serovars. Most of the floR-carrying Pasteurella multocida strains belonged to serovar D:L6 with significantly high resistance to some antimicrobial agents, especially florfenicol. Conclusions This study demonstrated that serovar D:L6 and multi-host ST10 was the most prevalent Pasteurella multocida strain in Taiwan. A:L6 accounted for the majority of toxA-positive strains and the presence of floR gene may be responsible for the antimicrobial resistance to florfenicol. |
| format | Article |
| id | doaj-art-939f04cd54734ac8856e50a087bcedb6 |
| institution | OA Journals |
| issn | 1746-6148 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
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| series | BMC Veterinary Research |
| spelling | doaj-art-939f04cd54734ac8856e50a087bcedb62025-08-20T02:16:40ZengBMCBMC Veterinary Research1746-61482025-02-0121111010.1186/s12917-025-04595-1Serovar and multilocus sequence typing analysis of Pasteurella multocida from diseased pigs in TaiwanChing-Fen Wu0Che-Cheng Liao1Chi-Chung Chou2Chao-Min Wang3Szu-Wei Huang4Hung-Chih Kuo5Department of Veterinary Medicine, National Chiayi UniversityDepartment of Veterinary Medicine, National Chiayi UniversityDepartment of Veterinary Medicine, National Chung Hsing UniversityDepartment of Veterinary Medicine, National Chiayi UniversityDepartment of Veterinary Medicine, National Chiayi UniversityDepartment of Veterinary Medicine, National Chiayi UniversityAbstract Background Pasteurella multocida causes progressive atrophic rhinitis and suppurative bronchopneumonia in pigs, which results in severe economic losses in swine industry. This study aimed to determine the serovar, genotype and prevalence of toxA virulence gene of Pasteurella multocida isolates collected in Taiwan. A total of 164 Pasteurella multocida isolates from 161 diseased pigs were characterized by serotyping, multilocus sequence typing (MLST), antimicrobial susceptibility testing, and the presence of virulence gene (toxA) and antibiotic resistance gene (floR). Results The majority of Pasteurella multocida strains were serovar D:L6 (48.2%; 79/164) followed by A:L6 (28.7%; 47/164) and A:L3 (19.5%; 32/164). More than 80% of strains carrying toxA gene belonged to serovar A:L6 (82.6%; 19/23). The MLST data showed five sequence types (STs), where multi-host ST10 was the most dominant. Most Pasteurella multocida strains of multi-host ST10 were serovar A:L6 (93.9%; 31/33), which suggested that STs were highly associated with specific serovars. Most of the floR-carrying Pasteurella multocida strains belonged to serovar D:L6 with significantly high resistance to some antimicrobial agents, especially florfenicol. Conclusions This study demonstrated that serovar D:L6 and multi-host ST10 was the most prevalent Pasteurella multocida strain in Taiwan. A:L6 accounted for the majority of toxA-positive strains and the presence of floR gene may be responsible for the antimicrobial resistance to florfenicol.https://doi.org/10.1186/s12917-025-04595-1Pasteurella multocidaMolecular serotypingMultilocus sequence typingAntimicrobial susceptibility testingfloR gene |
| spellingShingle | Ching-Fen Wu Che-Cheng Liao Chi-Chung Chou Chao-Min Wang Szu-Wei Huang Hung-Chih Kuo Serovar and multilocus sequence typing analysis of Pasteurella multocida from diseased pigs in Taiwan BMC Veterinary Research Pasteurella multocida Molecular serotyping Multilocus sequence typing Antimicrobial susceptibility testing floR gene |
| title | Serovar and multilocus sequence typing analysis of Pasteurella multocida from diseased pigs in Taiwan |
| title_full | Serovar and multilocus sequence typing analysis of Pasteurella multocida from diseased pigs in Taiwan |
| title_fullStr | Serovar and multilocus sequence typing analysis of Pasteurella multocida from diseased pigs in Taiwan |
| title_full_unstemmed | Serovar and multilocus sequence typing analysis of Pasteurella multocida from diseased pigs in Taiwan |
| title_short | Serovar and multilocus sequence typing analysis of Pasteurella multocida from diseased pigs in Taiwan |
| title_sort | serovar and multilocus sequence typing analysis of pasteurella multocida from diseased pigs in taiwan |
| topic | Pasteurella multocida Molecular serotyping Multilocus sequence typing Antimicrobial susceptibility testing floR gene |
| url | https://doi.org/10.1186/s12917-025-04595-1 |
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