Coordinated Dispersion and Aggregation of Gold Nanorod in Aptamer-Mediated Gestational Hypertension Analysis
Gestational hypertension is one of the complicated disorders during pregnancy; it causes the significant risks, such as placental abruption, neonatal deaths, and maternal deaths. Hypertension is also responsible for the metabolic and cardiovascular issues to the mother after the years of pregnancy....
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| Format: | Article |
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Wiley
2019-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2019/5676159 |
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| author | Xiucui Bao Gaoxiang Huo Li Li Xuebin Cao Yamei Liu Thangavel Lakshmipriya Yeng Chen Firdaus Hariri Subash C. B. Gopinath |
| author_facet | Xiucui Bao Gaoxiang Huo Li Li Xuebin Cao Yamei Liu Thangavel Lakshmipriya Yeng Chen Firdaus Hariri Subash C. B. Gopinath |
| author_sort | Xiucui Bao |
| collection | DOAJ |
| description | Gestational hypertension is one of the complicated disorders during pregnancy; it causes the significant risks, such as placental abruption, neonatal deaths, and maternal deaths. Hypertension is also responsible for the metabolic and cardiovascular issues to the mother after the years of pregnancy. Identifying and treating gestational hypertension during pregnancy by a suitable biomarker is mandatory for the healthy mother and foetus development. Cortisol has been found as a steroid hormone that is secreted by the adrenal gland and plays a pivotal role in gestational hypertension. A normal circulating level of cortisol is involved in the regulation of blood pressure, and it is necessary to monitor the changes in the level of cortisol during pregnancy. In this work, aptamer-based colorimetric assay is demonstrated as a model with gold nanorod to quantify the level of cortisol using the coordinated aggregation (at 500 mM of NaCl) and dispersion (with 10 μM of aptamer), evidenced by the scanning electron microscopy observation and UV-visible spectroscopy analysis. This colorimetric assay is an easier visual detection and reached the limit of detection of cortisol at 0.25 mg/mL. This method is reliable to identify the condition of gestational hypertension during the pregnancy period. |
| format | Article |
| id | doaj-art-93984eae62114b028a2b56bda472fac4 |
| institution | OA Journals |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-93984eae62114b028a2b56bda472fac42025-08-20T02:10:12ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732019-01-01201910.1155/2019/56761595676159Coordinated Dispersion and Aggregation of Gold Nanorod in Aptamer-Mediated Gestational Hypertension AnalysisXiucui Bao0Gaoxiang Huo1Li Li2Xuebin Cao3Yamei Liu4Thangavel Lakshmipriya5Yeng Chen6Firdaus Hariri7Subash C. B. Gopinath8Department of Obstetrics, Yihe Maternity District of Cangzhou People’s Hospital, Cangzhou, Hebei 061000, ChinaDepartment of Obstetrics, Yihe Maternity District of Cangzhou People’s Hospital, Cangzhou, Hebei 061000, ChinaDepartment of Obstetrics, Yihe Maternity District of Cangzhou People’s Hospital, Cangzhou, Hebei 061000, ChinaDepartment of General Surgery, Cangxian Hospital, Cangzhou, Hebei 061000, ChinaDepartment of Obstetrics, Yihe Maternity District of Cangzhou People’s Hospital, Cangzhou, Hebei 061000, ChinaInstitute of Nano Electronic Engineering, Universiti Malaysia Perlis, 01000 Kangar, Perlis, MalaysiaDepartment of Oral & Craniofacial Sciences, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, MalaysiaDepartment of Oral and Maxillofacial Clinical Sciences, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, MalaysiaInstitute of Nano Electronic Engineering, Universiti Malaysia Perlis, 01000 Kangar, Perlis, MalaysiaGestational hypertension is one of the complicated disorders during pregnancy; it causes the significant risks, such as placental abruption, neonatal deaths, and maternal deaths. Hypertension is also responsible for the metabolic and cardiovascular issues to the mother after the years of pregnancy. Identifying and treating gestational hypertension during pregnancy by a suitable biomarker is mandatory for the healthy mother and foetus development. Cortisol has been found as a steroid hormone that is secreted by the adrenal gland and plays a pivotal role in gestational hypertension. A normal circulating level of cortisol is involved in the regulation of blood pressure, and it is necessary to monitor the changes in the level of cortisol during pregnancy. In this work, aptamer-based colorimetric assay is demonstrated as a model with gold nanorod to quantify the level of cortisol using the coordinated aggregation (at 500 mM of NaCl) and dispersion (with 10 μM of aptamer), evidenced by the scanning electron microscopy observation and UV-visible spectroscopy analysis. This colorimetric assay is an easier visual detection and reached the limit of detection of cortisol at 0.25 mg/mL. This method is reliable to identify the condition of gestational hypertension during the pregnancy period.http://dx.doi.org/10.1155/2019/5676159 |
| spellingShingle | Xiucui Bao Gaoxiang Huo Li Li Xuebin Cao Yamei Liu Thangavel Lakshmipriya Yeng Chen Firdaus Hariri Subash C. B. Gopinath Coordinated Dispersion and Aggregation of Gold Nanorod in Aptamer-Mediated Gestational Hypertension Analysis Journal of Analytical Methods in Chemistry |
| title | Coordinated Dispersion and Aggregation of Gold Nanorod in Aptamer-Mediated Gestational Hypertension Analysis |
| title_full | Coordinated Dispersion and Aggregation of Gold Nanorod in Aptamer-Mediated Gestational Hypertension Analysis |
| title_fullStr | Coordinated Dispersion and Aggregation of Gold Nanorod in Aptamer-Mediated Gestational Hypertension Analysis |
| title_full_unstemmed | Coordinated Dispersion and Aggregation of Gold Nanorod in Aptamer-Mediated Gestational Hypertension Analysis |
| title_short | Coordinated Dispersion and Aggregation of Gold Nanorod in Aptamer-Mediated Gestational Hypertension Analysis |
| title_sort | coordinated dispersion and aggregation of gold nanorod in aptamer mediated gestational hypertension analysis |
| url | http://dx.doi.org/10.1155/2019/5676159 |
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