Tape Strip Profiling of Checkpoint Inhibitor–Associated Dermatitis Highlights Pan-T-Cell Activation: A Pilot Study

Immune checkpoint inhibitors (ICIs) have become mainstay therapy in the treatment of certain cancers. However, they are frequently associated with adverse effects in nontumor tissues. Cutaneous immune-related adverse events are the most prevalent toxicities, yet their underlying mechanisms remain po...

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Main Authors: Camille M. Powers, Madeline Kim, Annie Chang, Benjamin D. Hu, Brandon R. Block, Austin J. Piontkowski, Jeremy Orloff, Jade N. Young, Yeriel D. Estrada, Digpal S. Gour, Emma Guttman-Yassky, Nicholas Gulati
Format: Article
Language:English
Published: Elsevier 2025-07-01
Series:JID Innovations
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Online Access:http://www.sciencedirect.com/science/article/pii/S2667026725000311
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author Camille M. Powers
Madeline Kim
Annie Chang
Benjamin D. Hu
Brandon R. Block
Austin J. Piontkowski
Jeremy Orloff
Jade N. Young
Yeriel D. Estrada
Digpal S. Gour
Emma Guttman-Yassky
Nicholas Gulati
author_facet Camille M. Powers
Madeline Kim
Annie Chang
Benjamin D. Hu
Brandon R. Block
Austin J. Piontkowski
Jeremy Orloff
Jade N. Young
Yeriel D. Estrada
Digpal S. Gour
Emma Guttman-Yassky
Nicholas Gulati
author_sort Camille M. Powers
collection DOAJ
description Immune checkpoint inhibitors (ICIs) have become mainstay therapy in the treatment of certain cancers. However, they are frequently associated with adverse effects in nontumor tissues. Cutaneous immune-related adverse events are the most prevalent toxicities, yet their underlying mechanisms remain poorly understood. This pilot study investigated the molecular phenotype of ICI-associated eczematous dermatitis and ICI–associated lichen planus using minimally invasive tape strip sampling to compare these conditions with patients with atopic dermatitis and healthy controls. Transcriptomic analysis revealed significant T helper 1 upregulation in lesional ICI-associated eczematous dermatitis and ICI–associated lichen planus, surpassing the dysregulation seen in atopic dermatitis. T helper 2–related markers, including IL4R, were elevated in both ICI subtypes, aligning with prior clinical reports of dupilumab efficacy for cutaneous immune-related adverse events. Notably, JAK3 modulation was uniquely observed in lesional ICI-associated eczematous dermatitis. Lesional and nonlesional ICI–associated lichen planus demonstrated broad immune dysregulation, suggesting possible early inflammatory activity in seemingly unaffected skin. These findings highlight distinct immune pathway alterations in cutaneous immune-related adverse events compared with their ICI-independent counterparts, shedding light on potential therapeutic targets to manage these conditions without compromising ICI efficacy. Future studies in larger cohorts are warranted to validate these observations and evaluate targeted interventions for cutaneous immune-related adverse events.
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spelling doaj-art-9395917f756f4095b8a7902ffd9de7ec2025-08-20T03:34:00ZengElsevierJID Innovations2667-02672025-07-015410037510.1016/j.xjidi.2025.100375Tape Strip Profiling of Checkpoint Inhibitor–Associated Dermatitis Highlights Pan-T-Cell Activation: A Pilot StudyCamille M. Powers0Madeline Kim1Annie Chang2Benjamin D. Hu3Brandon R. Block4Austin J. Piontkowski5Jeremy Orloff6Jade N. Young7Yeriel D. Estrada8Digpal S. Gour9Emma Guttman-Yassky10Nicholas Gulati11Department of Dermatology, Icahn School of Medicine at Mount Sinai, New York, New York, USADepartment of Dermatology, Icahn School of Medicine at Mount Sinai, New York, New York, USADepartment of Dermatology, Icahn School of Medicine at Mount Sinai, New York, New York, USADepartment of Dermatology, Icahn School of Medicine at Mount Sinai, New York, New York, USADepartment of Dermatology, Icahn School of Medicine at Mount Sinai, New York, New York, USADepartment of Dermatology, Icahn School of Medicine at Mount Sinai, New York, New York, USADepartment of Dermatology, Icahn School of Medicine at Mount Sinai, New York, New York, USADepartment of Dermatology, Icahn School of Medicine at Mount Sinai, New York, New York, USADepartment of Dermatology, Icahn School of Medicine at Mount Sinai, New York, New York, USADepartment of Dermatology, Icahn School of Medicine at Mount Sinai, New York, New York, USADepartment of Dermatology, Icahn School of Medicine at Mount Sinai, New York, New York, USADepartment of Dermatology, Icahn School of Medicine at Mount Sinai, New York, New York, USA; Correspondence: Nicholas Gulati, Department of Dermatology, Icahn School of Medicine at Mount Sinai, 5 East 98th Street, 5th Floor, New York, New York 10029, USA.Immune checkpoint inhibitors (ICIs) have become mainstay therapy in the treatment of certain cancers. However, they are frequently associated with adverse effects in nontumor tissues. Cutaneous immune-related adverse events are the most prevalent toxicities, yet their underlying mechanisms remain poorly understood. This pilot study investigated the molecular phenotype of ICI-associated eczematous dermatitis and ICI–associated lichen planus using minimally invasive tape strip sampling to compare these conditions with patients with atopic dermatitis and healthy controls. Transcriptomic analysis revealed significant T helper 1 upregulation in lesional ICI-associated eczematous dermatitis and ICI–associated lichen planus, surpassing the dysregulation seen in atopic dermatitis. T helper 2–related markers, including IL4R, were elevated in both ICI subtypes, aligning with prior clinical reports of dupilumab efficacy for cutaneous immune-related adverse events. Notably, JAK3 modulation was uniquely observed in lesional ICI-associated eczematous dermatitis. Lesional and nonlesional ICI–associated lichen planus demonstrated broad immune dysregulation, suggesting possible early inflammatory activity in seemingly unaffected skin. These findings highlight distinct immune pathway alterations in cutaneous immune-related adverse events compared with their ICI-independent counterparts, shedding light on potential therapeutic targets to manage these conditions without compromising ICI efficacy. Future studies in larger cohorts are warranted to validate these observations and evaluate targeted interventions for cutaneous immune-related adverse events.http://www.sciencedirect.com/science/article/pii/S2667026725000311Atopic dermatitisCutaneous immune-related adverse eventsOncodermatologyRNA sequencingTape strips
spellingShingle Camille M. Powers
Madeline Kim
Annie Chang
Benjamin D. Hu
Brandon R. Block
Austin J. Piontkowski
Jeremy Orloff
Jade N. Young
Yeriel D. Estrada
Digpal S. Gour
Emma Guttman-Yassky
Nicholas Gulati
Tape Strip Profiling of Checkpoint Inhibitor–Associated Dermatitis Highlights Pan-T-Cell Activation: A Pilot Study
JID Innovations
Atopic dermatitis
Cutaneous immune-related adverse events
Oncodermatology
RNA sequencing
Tape strips
title Tape Strip Profiling of Checkpoint Inhibitor–Associated Dermatitis Highlights Pan-T-Cell Activation: A Pilot Study
title_full Tape Strip Profiling of Checkpoint Inhibitor–Associated Dermatitis Highlights Pan-T-Cell Activation: A Pilot Study
title_fullStr Tape Strip Profiling of Checkpoint Inhibitor–Associated Dermatitis Highlights Pan-T-Cell Activation: A Pilot Study
title_full_unstemmed Tape Strip Profiling of Checkpoint Inhibitor–Associated Dermatitis Highlights Pan-T-Cell Activation: A Pilot Study
title_short Tape Strip Profiling of Checkpoint Inhibitor–Associated Dermatitis Highlights Pan-T-Cell Activation: A Pilot Study
title_sort tape strip profiling of checkpoint inhibitor associated dermatitis highlights pan t cell activation a pilot study
topic Atopic dermatitis
Cutaneous immune-related adverse events
Oncodermatology
RNA sequencing
Tape strips
url http://www.sciencedirect.com/science/article/pii/S2667026725000311
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