Antifungal effect of soil Bacillus bacteria on pathogenic species of the fungal genera Aspergillus and Trichophyton
Background and Objectives: The increasing prevalence of fungal infections due to antifungal resistance underscores the need for novel treatment strategies. The present study aimed to investigate the inhibitory effects of soil-originated antagonistic bacteria against Aspergillus and Trichophyton spe...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2025-04-01
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| Series: | Iranian Journal of Microbiology |
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| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/5092 |
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| author | Mahnour Alsadat Taghavi Maryam Ahmadi Davoud Dehghan-Nayeri Zahra Salehi Masoomeh Shams-Ghahfarokhi Fatemehsadat Jamzivar Mehdi Razzaghi-Abyaneh |
| author_facet | Mahnour Alsadat Taghavi Maryam Ahmadi Davoud Dehghan-Nayeri Zahra Salehi Masoomeh Shams-Ghahfarokhi Fatemehsadat Jamzivar Mehdi Razzaghi-Abyaneh |
| author_sort | Mahnour Alsadat Taghavi |
| collection | DOAJ |
| description |
Background and Objectives: The increasing prevalence of fungal infections due to antifungal resistance underscores the need for novel treatment strategies. The present study aimed to investigate the inhibitory effects of soil-originated antagonistic bacteria against Aspergillus and Trichophyton species.
Materials and Methods: Fifty soil samples collected from Isfahan and Khuzestan provinces by using the Zig-Zag method were cultured on glucose-yeast extract (GY) agar around fungal colonies to isolate antagonistic bacteria. Antifungal activity was assessed by measuring clear zones around the colonies of A. niger, A. fumigatus, T. rubrum, and T. mentagrophytes by co-culture linear method. Potent antagonistic bacteria were identified by 16S rRNA sequencing, and evaluated for antifungal activity using disk diffusion assays compared with amphotericin B and ketoconazole.
Results: Among 50 samples, fifteen showed antifungal effects, yielding 55 bacterial strains. Four isolates with strong antifungal activity against all tested fungi were identified as Bacillus subtilis, B. licheniformis, B. axarquiensis, and Bacillus sp. These bacteria were distributed in distinct clusters phylogenitically and showed diverse antifungal activity.
Conclusion: The results suggest the potential of soil-derived Bacillus species as promising antifungal agents. Further studies are recommended to identify their inhibitory metabolites, their ability as biocontrol agents against soil habitated fungi and to explore their mechanism of action and spectrum of activity.
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| format | Article |
| id | doaj-art-939493904ea345eb89d14d3f3d760027 |
| institution | DOAJ |
| issn | 2008-3289 2008-4447 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj-art-939493904ea345eb89d14d3f3d7600272025-08-20T03:09:07ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472025-04-0117210.18502/ijm.v17i2.18397Antifungal effect of soil Bacillus bacteria on pathogenic species of the fungal genera Aspergillus and TrichophytonMahnour Alsadat Taghavi0Maryam Ahmadi1Davoud Dehghan-Nayeri2Zahra Salehi3Masoomeh Shams-Ghahfarokhi4Fatemehsadat Jamzivar5Mehdi Razzaghi-Abyaneh6Department of Microbiology, Faculty of Pharmacy, Islamic Azad University, Tehran, IranDepartment of Microbiology, Faculty of Pharmacy, Islamic Azad University, Tehran, IranDepartment of Microbiology, Faculty of Pharmacy, Islamic Azad University, Tehran, IranDepartment of Mycology, Pasteur Institute of Iran, Tehran, IranDepartmentt of Mycology, Faculty of Medical Science, Tarbiat Modares University, Tehran, IranDepartment of Mycology, Pasteur Institute of Iran, Tehran, IranDepartment of Mycology, Pasteur Institute of Iran, Tehran, Iran Background and Objectives: The increasing prevalence of fungal infections due to antifungal resistance underscores the need for novel treatment strategies. The present study aimed to investigate the inhibitory effects of soil-originated antagonistic bacteria against Aspergillus and Trichophyton species. Materials and Methods: Fifty soil samples collected from Isfahan and Khuzestan provinces by using the Zig-Zag method were cultured on glucose-yeast extract (GY) agar around fungal colonies to isolate antagonistic bacteria. Antifungal activity was assessed by measuring clear zones around the colonies of A. niger, A. fumigatus, T. rubrum, and T. mentagrophytes by co-culture linear method. Potent antagonistic bacteria were identified by 16S rRNA sequencing, and evaluated for antifungal activity using disk diffusion assays compared with amphotericin B and ketoconazole. Results: Among 50 samples, fifteen showed antifungal effects, yielding 55 bacterial strains. Four isolates with strong antifungal activity against all tested fungi were identified as Bacillus subtilis, B. licheniformis, B. axarquiensis, and Bacillus sp. These bacteria were distributed in distinct clusters phylogenitically and showed diverse antifungal activity. Conclusion: The results suggest the potential of soil-derived Bacillus species as promising antifungal agents. Further studies are recommended to identify their inhibitory metabolites, their ability as biocontrol agents against soil habitated fungi and to explore their mechanism of action and spectrum of activity. https://ijm.tums.ac.ir/index.php/ijm/article/view/5092Antifungal activity;Aspergillus;Trichophyton;Bacillus;Molecular identification;Soil bacteria |
| spellingShingle | Mahnour Alsadat Taghavi Maryam Ahmadi Davoud Dehghan-Nayeri Zahra Salehi Masoomeh Shams-Ghahfarokhi Fatemehsadat Jamzivar Mehdi Razzaghi-Abyaneh Antifungal effect of soil Bacillus bacteria on pathogenic species of the fungal genera Aspergillus and Trichophyton Iranian Journal of Microbiology Antifungal activity; Aspergillus; Trichophyton; Bacillus; Molecular identification; Soil bacteria |
| title | Antifungal effect of soil Bacillus bacteria on pathogenic species of the fungal genera Aspergillus and Trichophyton |
| title_full | Antifungal effect of soil Bacillus bacteria on pathogenic species of the fungal genera Aspergillus and Trichophyton |
| title_fullStr | Antifungal effect of soil Bacillus bacteria on pathogenic species of the fungal genera Aspergillus and Trichophyton |
| title_full_unstemmed | Antifungal effect of soil Bacillus bacteria on pathogenic species of the fungal genera Aspergillus and Trichophyton |
| title_short | Antifungal effect of soil Bacillus bacteria on pathogenic species of the fungal genera Aspergillus and Trichophyton |
| title_sort | antifungal effect of soil bacillus bacteria on pathogenic species of the fungal genera aspergillus and trichophyton |
| topic | Antifungal activity; Aspergillus; Trichophyton; Bacillus; Molecular identification; Soil bacteria |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/5092 |
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