A simple method of hiPSCs differentiation into insulin-producing cells is improved with vitamin C and RepSox.
Human induced pluripotent stem cells (hiPSCs) are considered a promising source of pancreatic β-cells for the treatment of diabetes. However, this approach is limited by issues such as low efficiency and high cost. Here, we have developed a new protocol to induce insulin-producing cells. To reduce c...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2021-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0254373&type=printable |
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| author | Ayumi Horikawa Keiko Mizuno Kyoko Tsuda Takayoshi Yamamoto Tatsuo Michiue |
| author_facet | Ayumi Horikawa Keiko Mizuno Kyoko Tsuda Takayoshi Yamamoto Tatsuo Michiue |
| author_sort | Ayumi Horikawa |
| collection | DOAJ |
| description | Human induced pluripotent stem cells (hiPSCs) are considered a promising source of pancreatic β-cells for the treatment of diabetes. However, this approach is limited by issues such as low efficiency and high cost. Here, we have developed a new protocol to induce insulin-producing cells. To reduce costs, we decreased the number of reagents and replaced protein reagents with chemical compounds. In this method, we increased induction efficiency with ascorbic acid (vitamin C) and an ALK5 inhibitor, RepSox. In 2D culture, the majority of cells were immature β-cells with low glucose-stimulated insulin secretion. Transferring to 3D culture immediately after endocrine progenitor cell differentiation, however, improved glucose-stimulated insulin secretion. This simplified method will contribute to realizing transplantation therapy of β-cells using iPSCs. |
| format | Article |
| id | doaj-art-938938cb370142d4a928cbde5fdd4754 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-938938cb370142d4a928cbde5fdd47542025-08-20T02:17:53ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-01167e025437310.1371/journal.pone.0254373A simple method of hiPSCs differentiation into insulin-producing cells is improved with vitamin C and RepSox.Ayumi HorikawaKeiko MizunoKyoko TsudaTakayoshi YamamotoTatsuo MichiueHuman induced pluripotent stem cells (hiPSCs) are considered a promising source of pancreatic β-cells for the treatment of diabetes. However, this approach is limited by issues such as low efficiency and high cost. Here, we have developed a new protocol to induce insulin-producing cells. To reduce costs, we decreased the number of reagents and replaced protein reagents with chemical compounds. In this method, we increased induction efficiency with ascorbic acid (vitamin C) and an ALK5 inhibitor, RepSox. In 2D culture, the majority of cells were immature β-cells with low glucose-stimulated insulin secretion. Transferring to 3D culture immediately after endocrine progenitor cell differentiation, however, improved glucose-stimulated insulin secretion. This simplified method will contribute to realizing transplantation therapy of β-cells using iPSCs.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0254373&type=printable |
| spellingShingle | Ayumi Horikawa Keiko Mizuno Kyoko Tsuda Takayoshi Yamamoto Tatsuo Michiue A simple method of hiPSCs differentiation into insulin-producing cells is improved with vitamin C and RepSox. PLoS ONE |
| title | A simple method of hiPSCs differentiation into insulin-producing cells is improved with vitamin C and RepSox. |
| title_full | A simple method of hiPSCs differentiation into insulin-producing cells is improved with vitamin C and RepSox. |
| title_fullStr | A simple method of hiPSCs differentiation into insulin-producing cells is improved with vitamin C and RepSox. |
| title_full_unstemmed | A simple method of hiPSCs differentiation into insulin-producing cells is improved with vitamin C and RepSox. |
| title_short | A simple method of hiPSCs differentiation into insulin-producing cells is improved with vitamin C and RepSox. |
| title_sort | simple method of hipscs differentiation into insulin producing cells is improved with vitamin c and repsox |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0254373&type=printable |
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