Insights on the enhancement of chilling tolerance in Rice through over-expression and knock-out studies of OsRBCS3
Chilling stress is an important environmental factor that affects rice (Oryza sativa L.) growth and yield, and the booting stage is the most sensitive stage of rice to chilling stress. In this study, we focused on OsRBCS3, a rice gene related to chilling tolerance at the booting stage, which encodes...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Plant Signaling & Behavior |
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| Online Access: | http://dx.doi.org/10.1080/15592324.2024.2318514 |
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| author | Yueting Hu Chongbing Tian Shiyu Song Rongtian Li |
| author_facet | Yueting Hu Chongbing Tian Shiyu Song Rongtian Li |
| author_sort | Yueting Hu |
| collection | DOAJ |
| description | Chilling stress is an important environmental factor that affects rice (Oryza sativa L.) growth and yield, and the booting stage is the most sensitive stage of rice to chilling stress. In this study, we focused on OsRBCS3, a rice gene related to chilling tolerance at the booting stage, which encodes the key enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) small subunit in photosynthesis. The aim of this study was to elucidate the role and mechanism of OsRBCS3 in rice chilling tolerance at the booting stage. The expression levels of OsRBCS3 under chilling stress were compared in two japonica rice cultivars with different chilling tolerances: Kongyu131 (KY131) and Longjing11 (LJ11). A positive correlation was found between OsRBCS3 expression and chilling tolerance. Over-expression (OE) and knock-out (KO) lines of OsRBCS3 were constructed using over-expression and CRISPR/Cas9 technology, respectively, and their chilling tolerance was evaluated at the seedling and booting stages. The results showed that OE lines exhibited higher chilling tolerance than wild-type (WT) lines at both seedling and booting stages, while KO lines showed lower chilling tolerance than WT lines. Furthermore, the antioxidant enzyme activities, malondialdehyde (MDA) content and Rubisco activity of four rice lines under chilling stress were measured, and it was found that OE lines had stronger antioxidant and photosynthetic capacities, while KO lines had the opposite effects. This study validated that OsRBCS3 plays an important role in rice chilling tolerance at the booting stage, providing new molecular tools and a theoretical basis for rice chilling tolerance breeding. |
| format | Article |
| id | doaj-art-9388beb9e38e4e56a483e2dca6dbc1a3 |
| institution | Kabale University |
| issn | 1559-2316 1559-2324 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
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| series | Plant Signaling & Behavior |
| spelling | doaj-art-9388beb9e38e4e56a483e2dca6dbc1a32025-01-02T11:44:26ZengTaylor & Francis GroupPlant Signaling & Behavior1559-23161559-23242024-12-0119110.1080/15592324.2024.23185142318514Insights on the enhancement of chilling tolerance in Rice through over-expression and knock-out studies of OsRBCS3Yueting Hu0Chongbing Tian1Shiyu Song2Rongtian Li3Heilongjiang Academy of Agricultural SciencesHeilongjiang Academy of Agricultural SciencesHeilongjiang UniversityHeilongjiang UniversityChilling stress is an important environmental factor that affects rice (Oryza sativa L.) growth and yield, and the booting stage is the most sensitive stage of rice to chilling stress. In this study, we focused on OsRBCS3, a rice gene related to chilling tolerance at the booting stage, which encodes the key enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) small subunit in photosynthesis. The aim of this study was to elucidate the role and mechanism of OsRBCS3 in rice chilling tolerance at the booting stage. The expression levels of OsRBCS3 under chilling stress were compared in two japonica rice cultivars with different chilling tolerances: Kongyu131 (KY131) and Longjing11 (LJ11). A positive correlation was found between OsRBCS3 expression and chilling tolerance. Over-expression (OE) and knock-out (KO) lines of OsRBCS3 were constructed using over-expression and CRISPR/Cas9 technology, respectively, and their chilling tolerance was evaluated at the seedling and booting stages. The results showed that OE lines exhibited higher chilling tolerance than wild-type (WT) lines at both seedling and booting stages, while KO lines showed lower chilling tolerance than WT lines. Furthermore, the antioxidant enzyme activities, malondialdehyde (MDA) content and Rubisco activity of four rice lines under chilling stress were measured, and it was found that OE lines had stronger antioxidant and photosynthetic capacities, while KO lines had the opposite effects. This study validated that OsRBCS3 plays an important role in rice chilling tolerance at the booting stage, providing new molecular tools and a theoretical basis for rice chilling tolerance breeding.http://dx.doi.org/10.1080/15592324.2024.2318514riceosrbcs3chilling toleranceover-expressionknock-out |
| spellingShingle | Yueting Hu Chongbing Tian Shiyu Song Rongtian Li Insights on the enhancement of chilling tolerance in Rice through over-expression and knock-out studies of OsRBCS3 Plant Signaling & Behavior rice osrbcs3 chilling tolerance over-expression knock-out |
| title | Insights on the enhancement of chilling tolerance in Rice through over-expression and knock-out studies of OsRBCS3 |
| title_full | Insights on the enhancement of chilling tolerance in Rice through over-expression and knock-out studies of OsRBCS3 |
| title_fullStr | Insights on the enhancement of chilling tolerance in Rice through over-expression and knock-out studies of OsRBCS3 |
| title_full_unstemmed | Insights on the enhancement of chilling tolerance in Rice through over-expression and knock-out studies of OsRBCS3 |
| title_short | Insights on the enhancement of chilling tolerance in Rice through over-expression and knock-out studies of OsRBCS3 |
| title_sort | insights on the enhancement of chilling tolerance in rice through over expression and knock out studies of osrbcs3 |
| topic | rice osrbcs3 chilling tolerance over-expression knock-out |
| url | http://dx.doi.org/10.1080/15592324.2024.2318514 |
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