Cloning and Characterization of a Flavonol Synthase Gene from Scutellaria baicalensis
Flavonols are the most abundant of all the flavonoids and play pivotal roles in a variety of plants. We isolated a cDNA clone encoding flavonol synthase from Scutellaria baicalensis (SbFLS). The SbFLS cDNA is 1011 bp long, encodes 336 amino acid residues, and belongs to a family of 2-oxoglutarate-de...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/980740 |
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| author | Yeon Bok Kim KwangSoo Kim YeJi Kim Pham Anh Tuan Haeng Hoon Kim Jin Woong Cho Sang Un Park |
| author_facet | Yeon Bok Kim KwangSoo Kim YeJi Kim Pham Anh Tuan Haeng Hoon Kim Jin Woong Cho Sang Un Park |
| author_sort | Yeon Bok Kim |
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| description | Flavonols are the most abundant of all the flavonoids and play pivotal roles in a variety of plants. We isolated a cDNA clone encoding flavonol synthase from Scutellaria baicalensis (SbFLS). The SbFLS cDNA is 1011 bp long, encodes 336 amino acid residues, and belongs to a family of 2-oxoglutarate-dependent dioxygenases. The overall structure of SbFLS is very similar to that of Arabidopsis thaliana anthocyanidin synthase (AtANS), with a β jelly-roll fold surrounded by tens of short and long α-helices. SbFLS was constitutively expressed in the roots, stems, leaves, and flowers, with particularly high expression in the roots and flowers. SbFLS transcript levels in the roots were 376-, 70-, and 2.5-fold higher than in the leaves, stems, and flowers. The myricetin content was significantly higher than that of kaempferol and quercetin. Therefore, we suggest that SbFLS mediates flavonol formation in the different organs of S. baicalensis. Our study may contribute to the knowledge of the role of FLS in S. baicalensis. |
| format | Article |
| id | doaj-art-937d363dbcc845299e28bb555eabe6c1 |
| institution | OA Journals |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-937d363dbcc845299e28bb555eabe6c12025-08-20T02:10:12ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/980740980740Cloning and Characterization of a Flavonol Synthase Gene from Scutellaria baicalensisYeon Bok Kim0KwangSoo Kim1YeJi Kim2Pham Anh Tuan3Haeng Hoon Kim4Jin Woong Cho5Sang Un Park6Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 305-764, Republic of KoreaDepartment of Biochemistry and Molecular Biology, Baylor College of Medicine One Baylor Plaza, Houston, TX 77030, USADepartment of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 305-764, Republic of KoreaDepartment of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 305-764, Republic of KoreaDepartment of Well-Being Resources, Sunchon National University, 413 Jungangno, Suncheon, Jeollanam-do 540-742, Republic of KoreaDepartment of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 305-764, Republic of KoreaDepartment of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 305-764, Republic of KoreaFlavonols are the most abundant of all the flavonoids and play pivotal roles in a variety of plants. We isolated a cDNA clone encoding flavonol synthase from Scutellaria baicalensis (SbFLS). The SbFLS cDNA is 1011 bp long, encodes 336 amino acid residues, and belongs to a family of 2-oxoglutarate-dependent dioxygenases. The overall structure of SbFLS is very similar to that of Arabidopsis thaliana anthocyanidin synthase (AtANS), with a β jelly-roll fold surrounded by tens of short and long α-helices. SbFLS was constitutively expressed in the roots, stems, leaves, and flowers, with particularly high expression in the roots and flowers. SbFLS transcript levels in the roots were 376-, 70-, and 2.5-fold higher than in the leaves, stems, and flowers. The myricetin content was significantly higher than that of kaempferol and quercetin. Therefore, we suggest that SbFLS mediates flavonol formation in the different organs of S. baicalensis. Our study may contribute to the knowledge of the role of FLS in S. baicalensis.http://dx.doi.org/10.1155/2014/980740 |
| spellingShingle | Yeon Bok Kim KwangSoo Kim YeJi Kim Pham Anh Tuan Haeng Hoon Kim Jin Woong Cho Sang Un Park Cloning and Characterization of a Flavonol Synthase Gene from Scutellaria baicalensis The Scientific World Journal |
| title | Cloning and Characterization of a Flavonol Synthase Gene from Scutellaria baicalensis |
| title_full | Cloning and Characterization of a Flavonol Synthase Gene from Scutellaria baicalensis |
| title_fullStr | Cloning and Characterization of a Flavonol Synthase Gene from Scutellaria baicalensis |
| title_full_unstemmed | Cloning and Characterization of a Flavonol Synthase Gene from Scutellaria baicalensis |
| title_short | Cloning and Characterization of a Flavonol Synthase Gene from Scutellaria baicalensis |
| title_sort | cloning and characterization of a flavonol synthase gene from scutellaria baicalensis |
| url | http://dx.doi.org/10.1155/2014/980740 |
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