Sugarcane: an unexpected habitat for black yeasts in Chaetothyriales
Abstract Sugarcane (Saccharum officinarum, Poaceae) is cultivated on a large scale in (sub)tropical regions such as Brazil and has considerable economic value for sugar and biofuel production. The plant is a rich substrate for endo- and epiphytic fungi. Black yeasts in the family Herpotrichiellaceae...
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BMC
2023-10-01
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| Online Access: | https://doi.org/10.1186/s43008-023-00124-7 |
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| author | Flávia de F. Costa Rafael S. C. de Souza Morgana F. Voidaleski Renata R. Gomes Guilherme F. Reis Bruna J. F. de S. Lima Giovanna Z. Candido Marlon R. Geraldo Jade M. B. Soares Gabriela X. Schneider Edvaldo da S. Trindade Israel H. Bini Leandro F. Moreno Amanda Bombassaro Flávio Queiroz-Telles Roberto T. Raittz Yu Quan Paulo Arruda Derlene Attili-Angelis Sybren de Hoog Vania A. Vicente |
| author_facet | Flávia de F. Costa Rafael S. C. de Souza Morgana F. Voidaleski Renata R. Gomes Guilherme F. Reis Bruna J. F. de S. Lima Giovanna Z. Candido Marlon R. Geraldo Jade M. B. Soares Gabriela X. Schneider Edvaldo da S. Trindade Israel H. Bini Leandro F. Moreno Amanda Bombassaro Flávio Queiroz-Telles Roberto T. Raittz Yu Quan Paulo Arruda Derlene Attili-Angelis Sybren de Hoog Vania A. Vicente |
| author_sort | Flávia de F. Costa |
| collection | DOAJ |
| description | Abstract Sugarcane (Saccharum officinarum, Poaceae) is cultivated on a large scale in (sub)tropical regions such as Brazil and has considerable economic value for sugar and biofuel production. The plant is a rich substrate for endo- and epiphytic fungi. Black yeasts in the family Herpotrichiellaceae (Chaetothyriales) are colonizers of human-dominated habitats, particularly those rich in toxins and hydrocarbon pollutants, and may cause severe infections in susceptible human hosts. The present study assessed the diversity of Herpotrichiellaceae associated with sugarcane, using in silico identification and selective isolation. Using metagenomics, we identified 5833 fungal sequences, while 639 black yeast-like isolates were recovered in vitro. In both strategies, the latter fungi were identified as members of the genera Cladophialophora, Exophiala, and Rhinocladiella (Herpotrichiellaceae), Cyphellophora (Cyphellophoraceae), and Knufia (Trichomeriaceae). In addition, we discovered new species of Cladophialophora and Exophiala from sugarcane and its rhizosphere. The first environmental isolation of Cladophialophora bantiana is particularly noteworthy, because this species up to now is exclusively known from the human host where it mostly causes fatal brain disease in otherwise healthy patients. |
| format | Article |
| id | doaj-art-937cbf728d644872b93a997407926d1c |
| institution | Kabale University |
| issn | 2210-6359 |
| language | English |
| publishDate | 2023-10-01 |
| publisher | BMC |
| record_format | Article |
| series | IMA Fungus |
| spelling | doaj-art-937cbf728d644872b93a997407926d1c2025-02-03T01:21:50ZengBMCIMA Fungus2210-63592023-10-0114112210.1186/s43008-023-00124-7Sugarcane: an unexpected habitat for black yeasts in ChaetothyrialesFlávia de F. Costa0Rafael S. C. de Souza1Morgana F. Voidaleski2Renata R. Gomes3Guilherme F. Reis4Bruna J. F. de S. Lima5Giovanna Z. Candido6Marlon R. Geraldo7Jade M. B. Soares8Gabriela X. Schneider9Edvaldo da S. Trindade10Israel H. Bini11Leandro F. Moreno12Amanda Bombassaro13Flávio Queiroz-Telles14Roberto T. Raittz15Yu Quan16Paulo Arruda17Derlene Attili-Angelis18Sybren de Hoog19Vania A. Vicente20Engineering Bioprocess and Biotechnology Post-Graduation Program, Department of Bioprocess Engineering and Biotechnology, Federal University of ParanáMolecular Biology and Genetics Engineering Center, State University of Campinas (UNICAMP)Microbiology, Parasitology and Pathology Post-Graduation Program, Department of Basic Pathology, Federal University of ParanáMicrobiology, Parasitology and Pathology Post-Graduation Program, Department of Basic Pathology, Federal University of ParanáEngineering Bioprocess and Biotechnology Post-Graduation Program, Department of Bioprocess Engineering and Biotechnology, Federal University of ParanáMicrobiology, Parasitology and Pathology Post-Graduation Program, Department of Basic Pathology, Federal University of ParanáMicrobiology, Parasitology and Pathology Post-Graduation Program, Department of Basic Pathology, Federal University of ParanáMicrobiology, Parasitology and Pathology Post-Graduation Program, Department of Basic Pathology, Federal University of ParanáBiological Sciences Graduation, Federal University of ParanáMicrobiology, Parasitology and Pathology Post-Graduation Program, Department of Basic Pathology, Federal University of ParanáDepartment of Cell Biology, Federal University of ParanáDepartment of Cell Biology, Federal University of ParanáMicrobiology, Parasitology and Pathology Post-Graduation Program, Department of Basic Pathology, Federal University of ParanáMicrobiology, Parasitology and Pathology Post-Graduation Program, Department of Basic Pathology, Federal University of ParanáMicrobiology, Parasitology and Pathology Post-Graduation Program, Department of Basic Pathology, Federal University of ParanáLaboratory of Bioinformatics, Professional and Technological Education Sector, Federal University of ParanáCenter of Expertise in Mycology of Radboud, University Medical Center / Canisius Wilhelmina HospitalMolecular Biology and Genetics Engineering Center, State University of Campinas (UNICAMP)Division of Microbial Resources (DRM/CPQBA), State University of Campinas (UNICAMP)Microbiology, Parasitology and Pathology Post-Graduation Program, Department of Basic Pathology, Federal University of ParanáEngineering Bioprocess and Biotechnology Post-Graduation Program, Department of Bioprocess Engineering and Biotechnology, Federal University of ParanáAbstract Sugarcane (Saccharum officinarum, Poaceae) is cultivated on a large scale in (sub)tropical regions such as Brazil and has considerable economic value for sugar and biofuel production. The plant is a rich substrate for endo- and epiphytic fungi. Black yeasts in the family Herpotrichiellaceae (Chaetothyriales) are colonizers of human-dominated habitats, particularly those rich in toxins and hydrocarbon pollutants, and may cause severe infections in susceptible human hosts. The present study assessed the diversity of Herpotrichiellaceae associated with sugarcane, using in silico identification and selective isolation. Using metagenomics, we identified 5833 fungal sequences, while 639 black yeast-like isolates were recovered in vitro. In both strategies, the latter fungi were identified as members of the genera Cladophialophora, Exophiala, and Rhinocladiella (Herpotrichiellaceae), Cyphellophora (Cyphellophoraceae), and Knufia (Trichomeriaceae). In addition, we discovered new species of Cladophialophora and Exophiala from sugarcane and its rhizosphere. The first environmental isolation of Cladophialophora bantiana is particularly noteworthy, because this species up to now is exclusively known from the human host where it mostly causes fatal brain disease in otherwise healthy patients.https://doi.org/10.1186/s43008-023-00124-7MetagenomicsSelective isolationCladophialophora bantiana |
| spellingShingle | Flávia de F. Costa Rafael S. C. de Souza Morgana F. Voidaleski Renata R. Gomes Guilherme F. Reis Bruna J. F. de S. Lima Giovanna Z. Candido Marlon R. Geraldo Jade M. B. Soares Gabriela X. Schneider Edvaldo da S. Trindade Israel H. Bini Leandro F. Moreno Amanda Bombassaro Flávio Queiroz-Telles Roberto T. Raittz Yu Quan Paulo Arruda Derlene Attili-Angelis Sybren de Hoog Vania A. Vicente Sugarcane: an unexpected habitat for black yeasts in Chaetothyriales IMA Fungus Metagenomics Selective isolation Cladophialophora bantiana |
| title | Sugarcane: an unexpected habitat for black yeasts in Chaetothyriales |
| title_full | Sugarcane: an unexpected habitat for black yeasts in Chaetothyriales |
| title_fullStr | Sugarcane: an unexpected habitat for black yeasts in Chaetothyriales |
| title_full_unstemmed | Sugarcane: an unexpected habitat for black yeasts in Chaetothyriales |
| title_short | Sugarcane: an unexpected habitat for black yeasts in Chaetothyriales |
| title_sort | sugarcane an unexpected habitat for black yeasts in chaetothyriales |
| topic | Metagenomics Selective isolation Cladophialophora bantiana |
| url | https://doi.org/10.1186/s43008-023-00124-7 |
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