Microfluidic techniques for separation of bacterial cells via taxis
The microbial environment is typically within a fluid and the key processes happen at the microscopic scale where viscosity dominates over inertial forces. Microfluidic tools are thus well suited to study microbial motility because they offer precise control of spatial structures and are ideal for t...
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| Format: | Article |
| Language: | English |
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Shared Science Publishers OG
2020-01-01
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| Series: | Microbial Cell |
| Subjects: | |
| Online Access: | http://microbialcell.com/researcharticles/2020a-gurung-microbial-cell/ |
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| _version_ | 1850225519378825216 |
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| author | Jyoti P. Gurung Murat Gel Matthew A. B. Baker |
| author_facet | Jyoti P. Gurung Murat Gel Matthew A. B. Baker |
| author_sort | Jyoti P. Gurung |
| collection | DOAJ |
| description | The microbial environment is typically within a fluid and the key processes happen at the microscopic scale where viscosity dominates over inertial forces. Microfluidic tools are thus well suited to study microbial motility because they offer precise control of spatial structures and are ideal for the generation of laminar fluid flows with low Reynolds numbers at microbial lengthscales. These tools have been used in combination with microscopy platforms to visualise and study various microbial taxes. These include establishing concentration and temperature gradients to influence motility via chemotaxis and thermotaxis, or controlling the surrounding microenvironment to influence rheotaxis, magnetotaxis, and phototaxis. Improvements in microfluidic technology have allowed fine separation of cells based on subtle differences in motility traits and have applications in synthetic biology, directed evolution, and applied medical microbiology. |
| format | Article |
| id | doaj-art-937bd808dfd24069b74c0384893ad8fa |
| institution | OA Journals |
| issn | 2311-2638 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Shared Science Publishers OG |
| record_format | Article |
| series | Microbial Cell |
| spelling | doaj-art-937bd808dfd24069b74c0384893ad8fa2025-08-20T02:05:20ZengShared Science Publishers OGMicrobial Cell2311-26382020-01-0173667910.15698/mic2020.03.710Microfluidic techniques for separation of bacterial cells via taxisJyoti P. Gurung0Murat Gel1Matthew A. B. Baker2School of Biotechnology and Biomolecular Science, UNSW Sydney.CSIRO Manufacturing, Clayton.School of Biotechnology and Biomolecular Science, UNSW Sydney.The microbial environment is typically within a fluid and the key processes happen at the microscopic scale where viscosity dominates over inertial forces. Microfluidic tools are thus well suited to study microbial motility because they offer precise control of spatial structures and are ideal for the generation of laminar fluid flows with low Reynolds numbers at microbial lengthscales. These tools have been used in combination with microscopy platforms to visualise and study various microbial taxes. These include establishing concentration and temperature gradients to influence motility via chemotaxis and thermotaxis, or controlling the surrounding microenvironment to influence rheotaxis, magnetotaxis, and phototaxis. Improvements in microfluidic technology have allowed fine separation of cells based on subtle differences in motility traits and have applications in synthetic biology, directed evolution, and applied medical microbiology.http://microbialcell.com/researcharticles/2020a-gurung-microbial-cell/flagellar motorchemotaxismicrofluidicsmotilitythermotaxis |
| spellingShingle | Jyoti P. Gurung Murat Gel Matthew A. B. Baker Microfluidic techniques for separation of bacterial cells via taxis Microbial Cell flagellar motor chemotaxis microfluidics motility thermotaxis |
| title | Microfluidic techniques for separation of bacterial cells via taxis |
| title_full | Microfluidic techniques for separation of bacterial cells via taxis |
| title_fullStr | Microfluidic techniques for separation of bacterial cells via taxis |
| title_full_unstemmed | Microfluidic techniques for separation of bacterial cells via taxis |
| title_short | Microfluidic techniques for separation of bacterial cells via taxis |
| title_sort | microfluidic techniques for separation of bacterial cells via taxis |
| topic | flagellar motor chemotaxis microfluidics motility thermotaxis |
| url | http://microbialcell.com/researcharticles/2020a-gurung-microbial-cell/ |
| work_keys_str_mv | AT jyotipgurung microfluidictechniquesforseparationofbacterialcellsviataxis AT muratgel microfluidictechniquesforseparationofbacterialcellsviataxis AT matthewabbaker microfluidictechniquesforseparationofbacterialcellsviataxis |