Modulating p56Lck in T-Cells by a Chimeric Peptide Comprising Two Functionally Different Motifs of Tip from Herpesvirus saimiri
The Lck interacting protein Tip of Herpesvirus saimiri is responsible for T-cell transformation both in vitro and in vivo. Here we designed the chimeric peptide hTip-CSKH, comprising the Lck specific interacting motif CSKH of Tip and its hydrophobic transmembrane sequence (hTip), the latter as a vec...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2015/395371 |
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| author | Jean-Paul Vernot Ana María Perdomo-Arciniegas Luis Alberto Pérez-Quintero Diego Fernando Martínez |
| author_facet | Jean-Paul Vernot Ana María Perdomo-Arciniegas Luis Alberto Pérez-Quintero Diego Fernando Martínez |
| author_sort | Jean-Paul Vernot |
| collection | DOAJ |
| description | The Lck interacting protein Tip of Herpesvirus saimiri is responsible for T-cell transformation both in vitro and in vivo. Here we designed the chimeric peptide hTip-CSKH, comprising the Lck specific interacting motif CSKH of Tip and its hydrophobic transmembrane sequence (hTip), the latter as a vector targeting lipid rafts. We found that hTip-CSKH can induce a fivefold increase in proliferation of human and Aotus sp. T-cells. Costimulation with PMA did not enhance this proliferation rate, suggesting that hTip-CSKH is sufficient and independent of further PKC stimulation. We also found that human Lck phosphorylation was increased earlier after stimulation when T-cells were incubated previously with hTip-CSKH, supporting a strong signalling and proliferative effect of the chimeric peptide. Additionally, Lck downstream signalling was evident with hTip-CSKH but not with control peptides. Importantly, hTip-CSKH could be identified in heavy lipid rafts membrane fractions, a compartment where important T-cell signalling molecules (LAT, Ras, and Lck) are present during T-cell activation. Interestingly, hTip-CSKH was inhibitory to Jurkat cells, in total agreement with the different signalling pathways and activation requirements of this leukemic cell line. These results provide the basis for the development of new compounds capable of modulating therapeutic targets present in lipid rafts. |
| format | Article |
| id | doaj-art-937abf0fa86b4b07b216fe26f0432796 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-937abf0fa86b4b07b216fe26f04327962025-02-03T01:31:26ZengWileyJournal of Immunology Research2314-88612314-71562015-01-01201510.1155/2015/395371395371Modulating p56Lck in T-Cells by a Chimeric Peptide Comprising Two Functionally Different Motifs of Tip from Herpesvirus saimiriJean-Paul Vernot0Ana María Perdomo-Arciniegas1Luis Alberto Pérez-Quintero2Diego Fernando Martínez3Fisiología Celular y Molecular, Facultad de Medicina, Universidad Nacional de Colombia, Bogotá 11001, ColombiaFisiología Celular y Molecular, Facultad de Medicina, Universidad Nacional de Colombia, Bogotá 11001, ColombiaFisiología Celular y Molecular, Facultad de Medicina, Universidad Nacional de Colombia, Bogotá 11001, ColombiaFisiología Celular y Molecular, Facultad de Medicina, Universidad Nacional de Colombia, Bogotá 11001, ColombiaThe Lck interacting protein Tip of Herpesvirus saimiri is responsible for T-cell transformation both in vitro and in vivo. Here we designed the chimeric peptide hTip-CSKH, comprising the Lck specific interacting motif CSKH of Tip and its hydrophobic transmembrane sequence (hTip), the latter as a vector targeting lipid rafts. We found that hTip-CSKH can induce a fivefold increase in proliferation of human and Aotus sp. T-cells. Costimulation with PMA did not enhance this proliferation rate, suggesting that hTip-CSKH is sufficient and independent of further PKC stimulation. We also found that human Lck phosphorylation was increased earlier after stimulation when T-cells were incubated previously with hTip-CSKH, supporting a strong signalling and proliferative effect of the chimeric peptide. Additionally, Lck downstream signalling was evident with hTip-CSKH but not with control peptides. Importantly, hTip-CSKH could be identified in heavy lipid rafts membrane fractions, a compartment where important T-cell signalling molecules (LAT, Ras, and Lck) are present during T-cell activation. Interestingly, hTip-CSKH was inhibitory to Jurkat cells, in total agreement with the different signalling pathways and activation requirements of this leukemic cell line. These results provide the basis for the development of new compounds capable of modulating therapeutic targets present in lipid rafts.http://dx.doi.org/10.1155/2015/395371 |
| spellingShingle | Jean-Paul Vernot Ana María Perdomo-Arciniegas Luis Alberto Pérez-Quintero Diego Fernando Martínez Modulating p56Lck in T-Cells by a Chimeric Peptide Comprising Two Functionally Different Motifs of Tip from Herpesvirus saimiri Journal of Immunology Research |
| title | Modulating p56Lck in T-Cells by a Chimeric Peptide Comprising Two Functionally Different Motifs of Tip from Herpesvirus saimiri |
| title_full | Modulating p56Lck in T-Cells by a Chimeric Peptide Comprising Two Functionally Different Motifs of Tip from Herpesvirus saimiri |
| title_fullStr | Modulating p56Lck in T-Cells by a Chimeric Peptide Comprising Two Functionally Different Motifs of Tip from Herpesvirus saimiri |
| title_full_unstemmed | Modulating p56Lck in T-Cells by a Chimeric Peptide Comprising Two Functionally Different Motifs of Tip from Herpesvirus saimiri |
| title_short | Modulating p56Lck in T-Cells by a Chimeric Peptide Comprising Two Functionally Different Motifs of Tip from Herpesvirus saimiri |
| title_sort | modulating p56lck in t cells by a chimeric peptide comprising two functionally different motifs of tip from herpesvirus saimiri |
| url | http://dx.doi.org/10.1155/2015/395371 |
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