Combatting glufosinate-induced pepper toxicity: jasmonic acid recruiting rhizosphere bacterial strain Rhodococcus gordoniae
Abstract Background Plant-microbe interactions are essential for mitigating abiotic and biotic stressors by shaping the rhizosphere environment. However, how rhizosphere beneficial bacteria and plant metabolites respond to glufosinate (GLU)-induced toxicity remains largely unknown. Results Our study...
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BMC
2025-07-01
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| Series: | Microbiome |
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| Online Access: | https://doi.org/10.1186/s40168-025-02155-1 |
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| author | Jialing Wang Ziyi Liu Xiaoyi Wang Zhijia Zhang Tianbing Zhou Mengmeng Li Shuai Wang Zhan Hu Ranfeng Sun Dong Li |
| author_facet | Jialing Wang Ziyi Liu Xiaoyi Wang Zhijia Zhang Tianbing Zhou Mengmeng Li Shuai Wang Zhan Hu Ranfeng Sun Dong Li |
| author_sort | Jialing Wang |
| collection | DOAJ |
| description | Abstract Background Plant-microbe interactions are essential for mitigating abiotic and biotic stressors by shaping the rhizosphere environment. However, how rhizosphere beneficial bacteria and plant metabolites respond to glufosinate (GLU)-induced toxicity remains largely unknown. Results Our study investigates the impact of GLU on chili plant growth and rhizosphere microbiome, emphasizing GLU-induced alterations in amino acid profiles, secondary metabolites, and microbial community composition, with notable enrichment of the Rhodococcus genus. To uncover the underlying mechanisms of Rhodococcus genus-root exudate interactions under GLU stress, we successfully isolated an efficient Rhodococcus gordoniae strain TR-5 from soil samples contaminated with GLU. This strain, isolated from GLU-contaminated soil, demonstrates potential for bioremediation and achieved over 95% GLU degradation efficiency at 35 °C, pH 6.38, and 1% inoculation rate. Through growth analysis, chemotaxis analysis, and molecular docking, caffeic acid disrupts the bacterial strain’s metabolic pathways and impedes TR-5 development. In contrast, jasmonic acid (JA) acts as a chemoattractant, promoting bacterial growth and metabolic activity to degrade GLU residues, thereby effectively degrading GLU residues in the soil. Conclusions This research indicates that GLU significantly influences the metabolic mechanisms of pepper plants. The optimization of microbial remediation strategies may improve soil remediation efficiency and reduce environmental impacts, highlighting opportunities for integrating microbial remediation into sustainable agricultural practices. Our findings provide insights into the role of JA in attracting and promoting the growth and metabolic activities of the Rhodococcus genus, which could be harnessed to improve soil remediation and plant health under GLU stress. Video Abstract Graphical Abstract |
| format | Article |
| id | doaj-art-937352f33a0d4213aa6f46174da8edd6 |
| institution | Kabale University |
| issn | 2049-2618 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbiome |
| spelling | doaj-art-937352f33a0d4213aa6f46174da8edd62025-08-20T03:45:34ZengBMCMicrobiome2049-26182025-07-0113112210.1186/s40168-025-02155-1Combatting glufosinate-induced pepper toxicity: jasmonic acid recruiting rhizosphere bacterial strain Rhodococcus gordoniaeJialing Wang0Ziyi Liu1Xiaoyi Wang2Zhijia Zhang3Tianbing Zhou4Mengmeng Li5Shuai Wang6Zhan Hu7Ranfeng Sun8Dong Li9Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, School of Tropical Agriculture and Forestry, Hainan University, Ministry of EducationKey Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, School of Tropical Agriculture and Forestry, Hainan University, Ministry of EducationKey Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, School of Tropical Agriculture and Forestry, Hainan University, Ministry of EducationKey Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, School of Tropical Agriculture and Forestry, Hainan University, Ministry of EducationKey Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, School of Tropical Agriculture and Forestry, Hainan University, Ministry of EducationKey Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, School of Tropical Agriculture and Forestry, Hainan University, Ministry of EducationKey Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, School of Tropical Agriculture and Forestry, Hainan University, Ministry of EducationKey Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, School of Tropical Agriculture and Forestry, Hainan University, Ministry of EducationKey Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, School of Tropical Agriculture and Forestry, Hainan University, Ministry of EducationKey Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, School of Tropical Agriculture and Forestry, Hainan University, Ministry of EducationAbstract Background Plant-microbe interactions are essential for mitigating abiotic and biotic stressors by shaping the rhizosphere environment. However, how rhizosphere beneficial bacteria and plant metabolites respond to glufosinate (GLU)-induced toxicity remains largely unknown. Results Our study investigates the impact of GLU on chili plant growth and rhizosphere microbiome, emphasizing GLU-induced alterations in amino acid profiles, secondary metabolites, and microbial community composition, with notable enrichment of the Rhodococcus genus. To uncover the underlying mechanisms of Rhodococcus genus-root exudate interactions under GLU stress, we successfully isolated an efficient Rhodococcus gordoniae strain TR-5 from soil samples contaminated with GLU. This strain, isolated from GLU-contaminated soil, demonstrates potential for bioremediation and achieved over 95% GLU degradation efficiency at 35 °C, pH 6.38, and 1% inoculation rate. Through growth analysis, chemotaxis analysis, and molecular docking, caffeic acid disrupts the bacterial strain’s metabolic pathways and impedes TR-5 development. In contrast, jasmonic acid (JA) acts as a chemoattractant, promoting bacterial growth and metabolic activity to degrade GLU residues, thereby effectively degrading GLU residues in the soil. Conclusions This research indicates that GLU significantly influences the metabolic mechanisms of pepper plants. The optimization of microbial remediation strategies may improve soil remediation efficiency and reduce environmental impacts, highlighting opportunities for integrating microbial remediation into sustainable agricultural practices. Our findings provide insights into the role of JA in attracting and promoting the growth and metabolic activities of the Rhodococcus genus, which could be harnessed to improve soil remediation and plant health under GLU stress. Video Abstract Graphical Abstracthttps://doi.org/10.1186/s40168-025-02155-1Glufosinate stressPlant growthBacterial communityRoot exudatesMicrobial remediation |
| spellingShingle | Jialing Wang Ziyi Liu Xiaoyi Wang Zhijia Zhang Tianbing Zhou Mengmeng Li Shuai Wang Zhan Hu Ranfeng Sun Dong Li Combatting glufosinate-induced pepper toxicity: jasmonic acid recruiting rhizosphere bacterial strain Rhodococcus gordoniae Microbiome Glufosinate stress Plant growth Bacterial community Root exudates Microbial remediation |
| title | Combatting glufosinate-induced pepper toxicity: jasmonic acid recruiting rhizosphere bacterial strain Rhodococcus gordoniae |
| title_full | Combatting glufosinate-induced pepper toxicity: jasmonic acid recruiting rhizosphere bacterial strain Rhodococcus gordoniae |
| title_fullStr | Combatting glufosinate-induced pepper toxicity: jasmonic acid recruiting rhizosphere bacterial strain Rhodococcus gordoniae |
| title_full_unstemmed | Combatting glufosinate-induced pepper toxicity: jasmonic acid recruiting rhizosphere bacterial strain Rhodococcus gordoniae |
| title_short | Combatting glufosinate-induced pepper toxicity: jasmonic acid recruiting rhizosphere bacterial strain Rhodococcus gordoniae |
| title_sort | combatting glufosinate induced pepper toxicity jasmonic acid recruiting rhizosphere bacterial strain rhodococcus gordoniae |
| topic | Glufosinate stress Plant growth Bacterial community Root exudates Microbial remediation |
| url | https://doi.org/10.1186/s40168-025-02155-1 |
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