Heracleum persicum L. extract protects gentamicin-induced testicular toxicity
Objective: The objective of this study was to assess the protective effects of Heracleum persicum L. leaves extract (HPE) against oxidative damage induced by gentamicin (GM) in the testes of rats through biochemical, histopathological, and immunohistochemical approaches. Materials and Methods: Thirt...
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Mashhad University of Medical Sciences
2024-09-01
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| Series: | Avicenna Journal of Phytomedicine |
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| Online Access: | https://ajp.mums.ac.ir/article_24347_f419cc4819aa436350d708d36a1e47a7.pdf |
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| author | Elnaz Khordad Mohsen Akbaribazm Seyed Moein Hosseini |
| author_facet | Elnaz Khordad Mohsen Akbaribazm Seyed Moein Hosseini |
| author_sort | Elnaz Khordad |
| collection | DOAJ |
| description | Objective: The objective of this study was to assess the protective effects of Heracleum persicum L. leaves extract (HPE) against oxidative damage induced by gentamicin (GM) in the testes of rats through biochemical, histopathological, and immunohistochemical approaches. Materials and Methods: Thirty-six male Wistar rats were divided into six groups (n=6/group) for 50 days. On day 51, the study assessed serum levels of luteinizing hormone (LH), follicle-stimulating hormone (FSH), and testosterone (TT), as well as antioxidant enzyme activity, nitric oxide levels, and various parameters related to testicular tissue (including the ferric reducing ability of plasma (FRAP), thiol, and thiobarbituric acid reactive substances (TBARS) levels). The stereological indices of seminiferous tubules were measured using serial sections of testicular tissue stained with hematoxylin and eosin, while the apoptosis rate of testicular parenchymal cells (p53, Caspase-3, and Bcl-2 positive cells) was also determined.Results: In the groups treated with HPE, particularly at 750 mg/kg, there was a significant increase (p<0.05) in LH, FSH, and TT hormone levels, an enhanced serum antioxidant enzyme activity and significantly reduced (p<0.05) nitric oxide levels. HPE inhibited the apoptotic pathway involving Bax/p53/Caspase-3 (significantly decreased (p<0.05) all three genes), thereby preserving the structure and function of the testicular tissue. Consequently, the number of p53 and Caspase-3 positive testicular cells decreased significantly (p<0.05), while the number of Bcl-2 positive cells increased. Conclusion: HPE demonstrated potential in protecting the function and structure of testis against toxic and oxidative damages. |
| format | Article |
| id | doaj-art-935a56ac002a4cc0a18f44a0a00b82c2 |
| institution | OA Journals |
| issn | 2228-7930 2228-7949 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Avicenna Journal of Phytomedicine |
| spelling | doaj-art-935a56ac002a4cc0a18f44a0a00b82c22025-08-20T02:15:24ZengMashhad University of Medical SciencesAvicenna Journal of Phytomedicine2228-79302228-79492024-09-0114558559910.22038/ajp.2024.2434724347Heracleum persicum L. extract protects gentamicin-induced testicular toxicityElnaz Khordad0Mohsen Akbaribazm1Seyed Moein Hosseini2Department of Anatomy, Torbat Heidariyeh University of Medical Sciences, Torbat Heidariyeh, IranDepartment of Anatomy, Torbat Heidariyeh University of Medical Sciences, Torbat Heidariyeh, IranStudent Research Committee, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, IranObjective: The objective of this study was to assess the protective effects of Heracleum persicum L. leaves extract (HPE) against oxidative damage induced by gentamicin (GM) in the testes of rats through biochemical, histopathological, and immunohistochemical approaches. Materials and Methods: Thirty-six male Wistar rats were divided into six groups (n=6/group) for 50 days. On day 51, the study assessed serum levels of luteinizing hormone (LH), follicle-stimulating hormone (FSH), and testosterone (TT), as well as antioxidant enzyme activity, nitric oxide levels, and various parameters related to testicular tissue (including the ferric reducing ability of plasma (FRAP), thiol, and thiobarbituric acid reactive substances (TBARS) levels). The stereological indices of seminiferous tubules were measured using serial sections of testicular tissue stained with hematoxylin and eosin, while the apoptosis rate of testicular parenchymal cells (p53, Caspase-3, and Bcl-2 positive cells) was also determined.Results: In the groups treated with HPE, particularly at 750 mg/kg, there was a significant increase (p<0.05) in LH, FSH, and TT hormone levels, an enhanced serum antioxidant enzyme activity and significantly reduced (p<0.05) nitric oxide levels. HPE inhibited the apoptotic pathway involving Bax/p53/Caspase-3 (significantly decreased (p<0.05) all three genes), thereby preserving the structure and function of the testicular tissue. Consequently, the number of p53 and Caspase-3 positive testicular cells decreased significantly (p<0.05), while the number of Bcl-2 positive cells increased. Conclusion: HPE demonstrated potential in protecting the function and structure of testis against toxic and oxidative damages.https://ajp.mums.ac.ir/article_24347_f419cc4819aa436350d708d36a1e47a7.pdfapoptosismale fertilityantioxidantgentamicinheracleum persicum l |
| spellingShingle | Elnaz Khordad Mohsen Akbaribazm Seyed Moein Hosseini Heracleum persicum L. extract protects gentamicin-induced testicular toxicity Avicenna Journal of Phytomedicine apoptosis male fertility antioxidant gentamicin heracleum persicum l |
| title | Heracleum persicum L. extract protects gentamicin-induced testicular toxicity |
| title_full | Heracleum persicum L. extract protects gentamicin-induced testicular toxicity |
| title_fullStr | Heracleum persicum L. extract protects gentamicin-induced testicular toxicity |
| title_full_unstemmed | Heracleum persicum L. extract protects gentamicin-induced testicular toxicity |
| title_short | Heracleum persicum L. extract protects gentamicin-induced testicular toxicity |
| title_sort | heracleum persicum l extract protects gentamicin induced testicular toxicity |
| topic | apoptosis male fertility antioxidant gentamicin heracleum persicum l |
| url | https://ajp.mums.ac.ir/article_24347_f419cc4819aa436350d708d36a1e47a7.pdf |
| work_keys_str_mv | AT elnazkhordad heracleumpersicumlextractprotectsgentamicininducedtesticulartoxicity AT mohsenakbaribazm heracleumpersicumlextractprotectsgentamicininducedtesticulartoxicity AT seyedmoeinhosseini heracleumpersicumlextractprotectsgentamicininducedtesticulartoxicity |