Screening for High-Yielding Pyruvate and Acetaldehyde Yeasts and Their Application in Improving the Stability of Anthocyanin in Mulberry Wine

The structure of anthocyanins in mulberry wine is highly unstable and, therefore, degrades during the fermentation and aging process. This instability hinders the maintenance of color, affects the sensory quality, and impedes the development of the mulberry wine industry. In this study, high-yieldin...

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Main Authors: Hui Zhou, Yajie Chai, Weidong Huang, Jicheng Zhan, Yilin You
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Foods
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Online Access:https://www.mdpi.com/2304-8158/14/13/2278
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author Hui Zhou
Yajie Chai
Weidong Huang
Jicheng Zhan
Yilin You
author_facet Hui Zhou
Yajie Chai
Weidong Huang
Jicheng Zhan
Yilin You
author_sort Hui Zhou
collection DOAJ
description The structure of anthocyanins in mulberry wine is highly unstable and, therefore, degrades during the fermentation and aging process. This instability hinders the maintenance of color, affects the sensory quality, and impedes the development of the mulberry wine industry. In this study, high-yielding acetaldehyde yeasts <i>Saccharomyces cerevisiae</i> GS32 and <i>Candida glabrata</i> GS13, as well as high-yielding pyruvate yeast <i>Candida xestobii</i> D1, were selected from our laboratory’s strain bank for application in mulberry wine fermentation. The objective was to determine the impact of these high-yielding yeasts on improving anthocyanin content and color stability. The results revealed that different strains and inoculation methods significantly influenced anthocyanin content and color stability in mulberry wine. The GS32 exhibited the highest increase in total anthocyanin content, while the D1 showed a superior retention rate for C3G (a specific type of anthocyanin). Over a period of 1–5 weeks of aging time, minimal changes in color were observed across all treatment groups. These findings suggest that fermenting with yeast strains that yield high levels of pyruvate and acetaldehyde is an effective approach to address issues related to the poor stability of anthocyanins in mulberry wine.
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spelling doaj-art-93570df2bb5844f9bd4af37e051c586b2025-08-20T03:16:43ZengMDPI AGFoods2304-81582025-06-011413227810.3390/foods14132278Screening for High-Yielding Pyruvate and Acetaldehyde Yeasts and Their Application in Improving the Stability of Anthocyanin in Mulberry WineHui Zhou0Yajie Chai1Weidong Huang2Jicheng Zhan3Yilin You4Yantai Institute of China Agricultural University, Binhai Middle Road 2006, Laishan District, Yantai 264670, ChinaYantai Institute of China Agricultural University, Binhai Middle Road 2006, Laishan District, Yantai 264670, ChinaYantai Institute of China Agricultural University, Binhai Middle Road 2006, Laishan District, Yantai 264670, ChinaYantai Institute of China Agricultural University, Binhai Middle Road 2006, Laishan District, Yantai 264670, ChinaYantai Institute of China Agricultural University, Binhai Middle Road 2006, Laishan District, Yantai 264670, ChinaThe structure of anthocyanins in mulberry wine is highly unstable and, therefore, degrades during the fermentation and aging process. This instability hinders the maintenance of color, affects the sensory quality, and impedes the development of the mulberry wine industry. In this study, high-yielding acetaldehyde yeasts <i>Saccharomyces cerevisiae</i> GS32 and <i>Candida glabrata</i> GS13, as well as high-yielding pyruvate yeast <i>Candida xestobii</i> D1, were selected from our laboratory’s strain bank for application in mulberry wine fermentation. The objective was to determine the impact of these high-yielding yeasts on improving anthocyanin content and color stability. The results revealed that different strains and inoculation methods significantly influenced anthocyanin content and color stability in mulberry wine. The GS32 exhibited the highest increase in total anthocyanin content, while the D1 showed a superior retention rate for C3G (a specific type of anthocyanin). Over a period of 1–5 weeks of aging time, minimal changes in color were observed across all treatment groups. These findings suggest that fermenting with yeast strains that yield high levels of pyruvate and acetaldehyde is an effective approach to address issues related to the poor stability of anthocyanins in mulberry wine.https://www.mdpi.com/2304-8158/14/13/2278wild wineyeastcolor stabilitypyruvate acidacetaldehydemulberry wine
spellingShingle Hui Zhou
Yajie Chai
Weidong Huang
Jicheng Zhan
Yilin You
Screening for High-Yielding Pyruvate and Acetaldehyde Yeasts and Their Application in Improving the Stability of Anthocyanin in Mulberry Wine
Foods
wild wineyeast
color stability
pyruvate acid
acetaldehyde
mulberry wine
title Screening for High-Yielding Pyruvate and Acetaldehyde Yeasts and Their Application in Improving the Stability of Anthocyanin in Mulberry Wine
title_full Screening for High-Yielding Pyruvate and Acetaldehyde Yeasts and Their Application in Improving the Stability of Anthocyanin in Mulberry Wine
title_fullStr Screening for High-Yielding Pyruvate and Acetaldehyde Yeasts and Their Application in Improving the Stability of Anthocyanin in Mulberry Wine
title_full_unstemmed Screening for High-Yielding Pyruvate and Acetaldehyde Yeasts and Their Application in Improving the Stability of Anthocyanin in Mulberry Wine
title_short Screening for High-Yielding Pyruvate and Acetaldehyde Yeasts and Their Application in Improving the Stability of Anthocyanin in Mulberry Wine
title_sort screening for high yielding pyruvate and acetaldehyde yeasts and their application in improving the stability of anthocyanin in mulberry wine
topic wild wineyeast
color stability
pyruvate acid
acetaldehyde
mulberry wine
url https://www.mdpi.com/2304-8158/14/13/2278
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