Enhanced sensitivity and efficiency of detection of Staphylococcus aureus based on modified magnetic nanoparticles by photometric systems
Staphylococcus aureus is an important infectious factor in the food industry and hospital infections. Many methods are used for detecting bacteria but they are mostly time-consuming, poorly sensitive. In this study, a nano-biosensor based on iron nanoparticles (MNPs) was designed to detect S. aureus...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2020.1748638 |
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| author | Ebrahim Naderlou Mojtaba Salouti Bahram Amini Ali Amini Asghar Narmani Ahmad Jalilvand Reza Shahbazi Saeid Zabihian |
| author_facet | Ebrahim Naderlou Mojtaba Salouti Bahram Amini Ali Amini Asghar Narmani Ahmad Jalilvand Reza Shahbazi Saeid Zabihian |
| author_sort | Ebrahim Naderlou |
| collection | DOAJ |
| description | Staphylococcus aureus is an important infectious factor in the food industry and hospital infections. Many methods are used for detecting bacteria but they are mostly time-consuming, poorly sensitive. In this study, a nano-biosensor based on iron nanoparticles (MNPs) was designed to detect S. aureus. MNPs were synthesized and conjugated to Biosensors. Then S. aureus was lysed and nano-biosensor (MNP-TiO2-AP-SMCC-Biosensors) was added to the lysed bacteria. After bonding the bacterial genome to the nano-biosensor, MNPs were separated by a magnet. Bacterial DNA was released from the surface of nano-biosensor and researched by Nano-drop spectrophotometry. The results of SEM and DLS revealed that the size of MNPs was 20–25 nm which increased to 38–43 nm after modification and addition of biosensors. The designed nano-biosensor was highly sensitive and specific for the detection of S. aureus. The limit of detection (LOD) was determined as 230 CFU mL−1. There was an acceptable linear correlation between bacterial concentration and absorption at 3.7 × 102–3.7× 107 whose linear diagram and regression was Y = 0.242X + 2.08 and R2 = .996. Further, in the presence of other bacteria as a negative control, it was absolutely specific. The sensitivity of the designed nano-biosensor was investigated and compared through PCR. |
| format | Article |
| id | doaj-art-934b6e2323ee40608149fa1c7f3c0cde |
| institution | Kabale University |
| issn | 2169-1401 2169-141X |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj-art-934b6e2323ee40608149fa1c7f3c0cde2025-08-20T04:02:41ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2020-01-0148181081710.1080/21691401.2020.1748638Enhanced sensitivity and efficiency of detection of Staphylococcus aureus based on modified magnetic nanoparticles by photometric systemsEbrahim Naderlou0Mojtaba Salouti1Bahram Amini2Ali Amini3Asghar Narmani4Ahmad Jalilvand5Reza Shahbazi6Saeid Zabihian7Faculty of Sciences, Department of Microbiology, Islamic Azad University, Zanjan, IranFaculty of Sciences, Department of Microbiology, Islamic Azad University, Zanjan, IranDepartment of Biochemistry, Zanjan University of Medical Sciences, Zanjan, IranDepartment of Biochemistry, Zanjan University of Medical Sciences, Zanjan, IranFaculty of New Sciences and Technologies, Department of Life Science Engineering, University of Tehran, Tehran, IranDepartment of Pathology, Zanjan University of Medical Sciences, Zanjan, IranFaculty of Sciences, Department of Microbiology, Islamic Azad University, Zanjan, IranDepartment of Pathology, Zanjan University of Medical Sciences, Zanjan, IranStaphylococcus aureus is an important infectious factor in the food industry and hospital infections. Many methods are used for detecting bacteria but they are mostly time-consuming, poorly sensitive. In this study, a nano-biosensor based on iron nanoparticles (MNPs) was designed to detect S. aureus. MNPs were synthesized and conjugated to Biosensors. Then S. aureus was lysed and nano-biosensor (MNP-TiO2-AP-SMCC-Biosensors) was added to the lysed bacteria. After bonding the bacterial genome to the nano-biosensor, MNPs were separated by a magnet. Bacterial DNA was released from the surface of nano-biosensor and researched by Nano-drop spectrophotometry. The results of SEM and DLS revealed that the size of MNPs was 20–25 nm which increased to 38–43 nm after modification and addition of biosensors. The designed nano-biosensor was highly sensitive and specific for the detection of S. aureus. The limit of detection (LOD) was determined as 230 CFU mL−1. There was an acceptable linear correlation between bacterial concentration and absorption at 3.7 × 102–3.7× 107 whose linear diagram and regression was Y = 0.242X + 2.08 and R2 = .996. Further, in the presence of other bacteria as a negative control, it was absolutely specific. The sensitivity of the designed nano-biosensor was investigated and compared through PCR.https://www.tandfonline.com/doi/10.1080/21691401.2020.1748638Nano-biosensorsMNPsStaphylococcus aureusdetectionnanoparticles |
| spellingShingle | Ebrahim Naderlou Mojtaba Salouti Bahram Amini Ali Amini Asghar Narmani Ahmad Jalilvand Reza Shahbazi Saeid Zabihian Enhanced sensitivity and efficiency of detection of Staphylococcus aureus based on modified magnetic nanoparticles by photometric systems Artificial Cells, Nanomedicine, and Biotechnology Nano-biosensors MNPs Staphylococcus aureus detection nanoparticles |
| title | Enhanced sensitivity and efficiency of detection of Staphylococcus aureus based on modified magnetic nanoparticles by photometric systems |
| title_full | Enhanced sensitivity and efficiency of detection of Staphylococcus aureus based on modified magnetic nanoparticles by photometric systems |
| title_fullStr | Enhanced sensitivity and efficiency of detection of Staphylococcus aureus based on modified magnetic nanoparticles by photometric systems |
| title_full_unstemmed | Enhanced sensitivity and efficiency of detection of Staphylococcus aureus based on modified magnetic nanoparticles by photometric systems |
| title_short | Enhanced sensitivity and efficiency of detection of Staphylococcus aureus based on modified magnetic nanoparticles by photometric systems |
| title_sort | enhanced sensitivity and efficiency of detection of staphylococcus aureus based on modified magnetic nanoparticles by photometric systems |
| topic | Nano-biosensors MNPs Staphylococcus aureus detection nanoparticles |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2020.1748638 |
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