Localizing axial dense emitters based on single-helix point spread function and compressed sensing
Among the approaches in three-dimensional (3D) single molecule localization microscopy, there are several point spread function (PSF) engineering approaches, in which depth information of molecules is encoded in 2D images. Usually, the molecules are excited sparsely in each raw image. The consequenc...
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| Format: | Article |
| Language: | English |
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De Gruyter
2025-02-01
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| Series: | Nanophotonics |
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| Online Access: | https://doi.org/10.1515/nanoph-2024-0516 |
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| author | Wu Hanzhe Chen Danni Ji Yihong Xiang Gan Ni Yanxiang Li Heng Yu Bin Qu Junle |
| author_facet | Wu Hanzhe Chen Danni Ji Yihong Xiang Gan Ni Yanxiang Li Heng Yu Bin Qu Junle |
| author_sort | Wu Hanzhe |
| collection | DOAJ |
| description | Among the approaches in three-dimensional (3D) single molecule localization microscopy, there are several point spread function (PSF) engineering approaches, in which depth information of molecules is encoded in 2D images. Usually, the molecules are excited sparsely in each raw image. The consequence is that the temporal resolution has to be sacrificed. In order to improve temporal resolution and ensure localization accuracy, we propose a method, SH-CS, based on light needle excitation, detection system with single helix-point spread function (SH-PSF), and compressed sensing (CS). Although the SH-CS method still has a limitation about the molecule density, it is suited for relatively dense molecules. For each light needle scanning position, an SH image of excited molecules is processed with CS algorithm to decode their axial information. Simulations demonstrated, for random distributed 1–15 molecules in depth range of 4 μm, the axial localization accuracy is 12.1–73.5 nm. The feasibility of this method is validated with a designed 3D sample composed of fluorescent beads. |
| format | Article |
| id | doaj-art-9343ffd5cef047bb808056e5b0ebbb8e |
| institution | DOAJ |
| issn | 2192-8614 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Nanophotonics |
| spelling | doaj-art-9343ffd5cef047bb808056e5b0ebbb8e2025-08-20T03:01:57ZengDe GruyterNanophotonics2192-86142025-02-0114453554310.1515/nanoph-2024-0516Localizing axial dense emitters based on single-helix point spread function and compressed sensingWu Hanzhe0Chen Danni1Ji Yihong2Xiang Gan3Ni Yanxiang4Li Heng5Yu Bin6Qu Junle7Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education, College of Physics and Optoelectronic Engineering, 47890Shenzhen University, Shenzhen518060, Guangdong Province, ChinaKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education, College of Physics and Optoelectronic Engineering, 47890Shenzhen University, Shenzhen518060, Guangdong Province, ChinaKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education, College of Physics and Optoelectronic Engineering, 47890Shenzhen University, Shenzhen518060, Guangdong Province, ChinaKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education, College of Physics and Optoelectronic Engineering, 47890Shenzhen University, Shenzhen518060, Guangdong Province, ChinaKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education, College of Physics and Optoelectronic Engineering, 47890Shenzhen University, Shenzhen518060, Guangdong Province, ChinaTsinghua-Berkeley Shenzhen Institute (TBSI), Tsinghua University, Shenzhen, 518055, ChinaKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education, College of Physics and Optoelectronic Engineering, 47890Shenzhen University, Shenzhen518060, Guangdong Province, ChinaKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education, College of Physics and Optoelectronic Engineering, 47890Shenzhen University, Shenzhen518060, Guangdong Province, ChinaAmong the approaches in three-dimensional (3D) single molecule localization microscopy, there are several point spread function (PSF) engineering approaches, in which depth information of molecules is encoded in 2D images. Usually, the molecules are excited sparsely in each raw image. The consequence is that the temporal resolution has to be sacrificed. In order to improve temporal resolution and ensure localization accuracy, we propose a method, SH-CS, based on light needle excitation, detection system with single helix-point spread function (SH-PSF), and compressed sensing (CS). Although the SH-CS method still has a limitation about the molecule density, it is suited for relatively dense molecules. For each light needle scanning position, an SH image of excited molecules is processed with CS algorithm to decode their axial information. Simulations demonstrated, for random distributed 1–15 molecules in depth range of 4 μm, the axial localization accuracy is 12.1–73.5 nm. The feasibility of this method is validated with a designed 3D sample composed of fluorescent beads.https://doi.org/10.1515/nanoph-2024-0516three-dimensional single molecule localization microscopycompressed sensingsingle-helix point spread function |
| spellingShingle | Wu Hanzhe Chen Danni Ji Yihong Xiang Gan Ni Yanxiang Li Heng Yu Bin Qu Junle Localizing axial dense emitters based on single-helix point spread function and compressed sensing Nanophotonics three-dimensional single molecule localization microscopy compressed sensing single-helix point spread function |
| title | Localizing axial dense emitters based on single-helix point spread function and compressed sensing |
| title_full | Localizing axial dense emitters based on single-helix point spread function and compressed sensing |
| title_fullStr | Localizing axial dense emitters based on single-helix point spread function and compressed sensing |
| title_full_unstemmed | Localizing axial dense emitters based on single-helix point spread function and compressed sensing |
| title_short | Localizing axial dense emitters based on single-helix point spread function and compressed sensing |
| title_sort | localizing axial dense emitters based on single helix point spread function and compressed sensing |
| topic | three-dimensional single molecule localization microscopy compressed sensing single-helix point spread function |
| url | https://doi.org/10.1515/nanoph-2024-0516 |
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