Isolation and characterization of potential amylolytic, proteolytic, gelatinolytic and chitinolytic properties in bacterial antagonists obtained from chilli plants (Colletotrichum spp.)
Several bacteria are found to be potential as antagonists due to their capacity in producing extracellular enzymes amylase, protease, gelatinase, cellulose and chitinase. In this research, we collected bacteria from both healthy and anthracnose-infecting chili plants (root, stem and leaf). The resul...
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| Format: | Article |
| Language: | English |
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EDP Sciences
2025-01-01
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| Series: | BIO Web of Conferences |
| Online Access: | https://www.bio-conferences.org/articles/bioconf/pdf/2025/09/bioconf_icsard2024_03021.pdf |
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| author | Tarigan Rasiska Lisnawita Lisnawita Tantawi A.R. Hermanto Catur |
| author_facet | Tarigan Rasiska Lisnawita Lisnawita Tantawi A.R. Hermanto Catur |
| author_sort | Tarigan Rasiska |
| collection | DOAJ |
| description | Several bacteria are found to be potential as antagonists due to their capacity in producing extracellular enzymes amylase, protease, gelatinase, cellulose and chitinase. In this research, we collected bacteria from both healthy and anthracnose-infecting chili plants (root, stem and leaf). The results revealed that 27 bacterial isolates are non-pathogenic and non-hemolytic to plants, which are 10 bacteria isolated from roots, 8 bacteria isolated from leaves, 7 bacteria isolated from stems and 2 bacteria isolated from fruits. Morphology identification showed that most of non-pathogenic bacteria are circular, irregular and punctiform, with elevation flat, raised and umbonate. White and milky bacteria are dominant, with varied opacity (transparent, translucent and opaque) and textures (slimy, mucoid, shiny and dry). Enzymatic activity test revealed 11 isolates produced protease, amylase and chitinase enzymes (isolates 11, 34, 42, 48, 111,118, 139, 188, 215, 216 and 218). |
| format | Article |
| id | doaj-art-93382f1eb3be490ebfbe0282c6b2316a |
| institution | OA Journals |
| issn | 2117-4458 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | BIO Web of Conferences |
| spelling | doaj-art-93382f1eb3be490ebfbe0282c6b2316a2025-08-20T02:13:41ZengEDP SciencesBIO Web of Conferences2117-44582025-01-011580302110.1051/bioconf/202515803021bioconf_icsard2024_03021Isolation and characterization of potential amylolytic, proteolytic, gelatinolytic and chitinolytic properties in bacterial antagonists obtained from chilli plants (Colletotrichum spp.)Tarigan Rasiska0Lisnawita Lisnawita1Tantawi A.R.2Hermanto Catur3Research Center for Horticulture, National Research and Innovation AgencyDepartment of Agrotechnology, Faculty of Agriculture, Universitas Medan AreaDepartment of Agrotechnology, Faculty of Agriculture, Universitas Medan AreaResearch Center for Horticulture, National Research and Innovation AgencySeveral bacteria are found to be potential as antagonists due to their capacity in producing extracellular enzymes amylase, protease, gelatinase, cellulose and chitinase. In this research, we collected bacteria from both healthy and anthracnose-infecting chili plants (root, stem and leaf). The results revealed that 27 bacterial isolates are non-pathogenic and non-hemolytic to plants, which are 10 bacteria isolated from roots, 8 bacteria isolated from leaves, 7 bacteria isolated from stems and 2 bacteria isolated from fruits. Morphology identification showed that most of non-pathogenic bacteria are circular, irregular and punctiform, with elevation flat, raised and umbonate. White and milky bacteria are dominant, with varied opacity (transparent, translucent and opaque) and textures (slimy, mucoid, shiny and dry). Enzymatic activity test revealed 11 isolates produced protease, amylase and chitinase enzymes (isolates 11, 34, 42, 48, 111,118, 139, 188, 215, 216 and 218).https://www.bio-conferences.org/articles/bioconf/pdf/2025/09/bioconf_icsard2024_03021.pdf |
| spellingShingle | Tarigan Rasiska Lisnawita Lisnawita Tantawi A.R. Hermanto Catur Isolation and characterization of potential amylolytic, proteolytic, gelatinolytic and chitinolytic properties in bacterial antagonists obtained from chilli plants (Colletotrichum spp.) BIO Web of Conferences |
| title | Isolation and characterization of potential amylolytic, proteolytic, gelatinolytic and chitinolytic properties in bacterial antagonists obtained from chilli plants (Colletotrichum spp.) |
| title_full | Isolation and characterization of potential amylolytic, proteolytic, gelatinolytic and chitinolytic properties in bacterial antagonists obtained from chilli plants (Colletotrichum spp.) |
| title_fullStr | Isolation and characterization of potential amylolytic, proteolytic, gelatinolytic and chitinolytic properties in bacterial antagonists obtained from chilli plants (Colletotrichum spp.) |
| title_full_unstemmed | Isolation and characterization of potential amylolytic, proteolytic, gelatinolytic and chitinolytic properties in bacterial antagonists obtained from chilli plants (Colletotrichum spp.) |
| title_short | Isolation and characterization of potential amylolytic, proteolytic, gelatinolytic and chitinolytic properties in bacterial antagonists obtained from chilli plants (Colletotrichum spp.) |
| title_sort | isolation and characterization of potential amylolytic proteolytic gelatinolytic and chitinolytic properties in bacterial antagonists obtained from chilli plants colletotrichum spp |
| url | https://www.bio-conferences.org/articles/bioconf/pdf/2025/09/bioconf_icsard2024_03021.pdf |
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