Oxylipins in Aqueous Humor of Primary Open-Angle Glaucoma Patients
Purpose: Investigate the oxylipin profiles in the aqueous humor of primary open-angle glaucoma (POAG) patients. Methods: Aqueous humor samples were collected from 17 POAG patients and 15 cataract subjects and subjected to a liquid chromatography/mass spectrometry (LC-MS) analysis to detect the oxyli...
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2024-09-01
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| Series: | Biomolecules |
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| author | Jianming Xu Kewen Zhou Changzhen Fu Chong-Bo Chen Yaru Sun Xin Wen Luxi Yang Tsz-Kin Ng Qingping Liu Mingzhi Zhang |
| author_facet | Jianming Xu Kewen Zhou Changzhen Fu Chong-Bo Chen Yaru Sun Xin Wen Luxi Yang Tsz-Kin Ng Qingping Liu Mingzhi Zhang |
| author_sort | Jianming Xu |
| collection | DOAJ |
| description | Purpose: Investigate the oxylipin profiles in the aqueous humor of primary open-angle glaucoma (POAG) patients. Methods: Aqueous humor samples were collected from 17 POAG patients and 15 cataract subjects and subjected to a liquid chromatography/mass spectrometry (LC-MS) analysis to detect the oxylipins. The prediction potential of the differential abundant oxylipins was assessed by the receiver operating characteristic (ROC) curves. Pathway and correlation analyses on the oxylipins and clinical and biochemical parameters were also conducted. Results: The LC-MS analysis detected a total of 76 oxylipins, of which 29 oxylipins reached the detection limit. The multivariate analysis identified five differential abundant oxylipins, 15-keto-prostaglandin F2 alpha (15-kPGF2α), Leukotriene B4 (LTB4), 12,13-Epoxyoctadecenoic acid (12,13-Epome), 15-Hydroxyeicosatetraenoic acid (15-HETE) and 11-Hydroxyeicosatetraenoic acid (11-HETE). The five oxylipins are enriched in the arachidonic acid metabolism and linoleic acid metabolism pathways. Pearson correlation analysis showed that 11-HETE was positively correlated with intraocular pressure and central corneal thickness and negatively with cup/disk area ratio in the POAG patients. In addition, 15-kPGF2α was moderately and positively correlated with the mean deviation (MD) of visual field defect, and LTB4 was moderately and negatively correlated with macular thickness. Conclusions: This study revealed the oxylipin profile in the aqueous humor of POAG patients. Oxylipins involved in the arachidonic acid metabolism pathway could play a role in POAG, and anti-inflammatory therapies could be potential treatment strategies for POAG. |
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| language | English |
| publishDate | 2024-09-01 |
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| series | Biomolecules |
| spelling | doaj-art-933499e1d14745a18acbfdf4701e8d752025-08-20T01:56:11ZengMDPI AGBiomolecules2218-273X2024-09-01149112710.3390/biom14091127Oxylipins in Aqueous Humor of Primary Open-Angle Glaucoma PatientsJianming Xu0Kewen Zhou1Changzhen Fu2Chong-Bo Chen3Yaru Sun4Xin Wen5Luxi Yang6Tsz-Kin Ng7Qingping Liu8Mingzhi Zhang9Joint Shantou International Eye Center of Shantou University and The Chinese University of Hong Kong, Shantou 515041, ChinaJoint Shantou International Eye Center of Shantou University and The Chinese University of Hong Kong, Shantou 515041, ChinaJoint Shantou International Eye Center of Shantou University and The Chinese University of Hong Kong, Shantou 515041, ChinaJoint Shantou International Eye Center of Shantou University and The Chinese University of Hong Kong, Shantou 515041, ChinaJoint Shantou International Eye Center of Shantou University and The Chinese University of Hong Kong, Shantou 515041, ChinaJoint Shantou International Eye Center of Shantou University and The Chinese University of Hong Kong, Shantou 515041, ChinaJoint Shantou International Eye Center of Shantou University and The Chinese University of Hong Kong, Shantou 515041, ChinaJoint Shantou International Eye Center of Shantou University and The Chinese University of Hong Kong, Shantou 515041, ChinaJoint Shantou International Eye Center of Shantou University and The Chinese University of Hong Kong, Shantou 515041, ChinaJoint Shantou International Eye Center of Shantou University and The Chinese University of Hong Kong, Shantou 515041, ChinaPurpose: Investigate the oxylipin profiles in the aqueous humor of primary open-angle glaucoma (POAG) patients. Methods: Aqueous humor samples were collected from 17 POAG patients and 15 cataract subjects and subjected to a liquid chromatography/mass spectrometry (LC-MS) analysis to detect the oxylipins. The prediction potential of the differential abundant oxylipins was assessed by the receiver operating characteristic (ROC) curves. Pathway and correlation analyses on the oxylipins and clinical and biochemical parameters were also conducted. Results: The LC-MS analysis detected a total of 76 oxylipins, of which 29 oxylipins reached the detection limit. The multivariate analysis identified five differential abundant oxylipins, 15-keto-prostaglandin F2 alpha (15-kPGF2α), Leukotriene B4 (LTB4), 12,13-Epoxyoctadecenoic acid (12,13-Epome), 15-Hydroxyeicosatetraenoic acid (15-HETE) and 11-Hydroxyeicosatetraenoic acid (11-HETE). The five oxylipins are enriched in the arachidonic acid metabolism and linoleic acid metabolism pathways. Pearson correlation analysis showed that 11-HETE was positively correlated with intraocular pressure and central corneal thickness and negatively with cup/disk area ratio in the POAG patients. In addition, 15-kPGF2α was moderately and positively correlated with the mean deviation (MD) of visual field defect, and LTB4 was moderately and negatively correlated with macular thickness. Conclusions: This study revealed the oxylipin profile in the aqueous humor of POAG patients. Oxylipins involved in the arachidonic acid metabolism pathway could play a role in POAG, and anti-inflammatory therapies could be potential treatment strategies for POAG.https://www.mdpi.com/2218-273X/14/9/1127primary open-angle glaucomaaqueous humoroxylipinsarachidonic acid |
| spellingShingle | Jianming Xu Kewen Zhou Changzhen Fu Chong-Bo Chen Yaru Sun Xin Wen Luxi Yang Tsz-Kin Ng Qingping Liu Mingzhi Zhang Oxylipins in Aqueous Humor of Primary Open-Angle Glaucoma Patients Biomolecules primary open-angle glaucoma aqueous humor oxylipins arachidonic acid |
| title | Oxylipins in Aqueous Humor of Primary Open-Angle Glaucoma Patients |
| title_full | Oxylipins in Aqueous Humor of Primary Open-Angle Glaucoma Patients |
| title_fullStr | Oxylipins in Aqueous Humor of Primary Open-Angle Glaucoma Patients |
| title_full_unstemmed | Oxylipins in Aqueous Humor of Primary Open-Angle Glaucoma Patients |
| title_short | Oxylipins in Aqueous Humor of Primary Open-Angle Glaucoma Patients |
| title_sort | oxylipins in aqueous humor of primary open angle glaucoma patients |
| topic | primary open-angle glaucoma aqueous humor oxylipins arachidonic acid |
| url | https://www.mdpi.com/2218-273X/14/9/1127 |
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