Spatial and temporal distribution of p21 senescent cells in periodontal tissue of diabetic mice
[Objective:] To investigate the spatial and temporal distribution of p21 senescent cells in periodontal tissue and femur of diabetic mice. [Methods:] We constructed transgenic mice capable of tracing p21 senescent cells, used gel electrophoresis assay to screen 24 homozygous mice, and randomly divid...
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Editorial Office of Journal of Oral and Maxillofacial Surgery
2024-12-01
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| Series: | Kouqiang hemian waike zazhi |
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| Online Access: | https://journal06.magtech.org.cn/Jweb_joms/EN/10.12439/kqhm.1005-4979.2024.06.004 |
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| author | YI Zimei REN Le WEI Yu CHEN Siyi YAN Jiayu WU Junhua |
| author_facet | YI Zimei REN Le WEI Yu CHEN Siyi YAN Jiayu WU Junhua |
| author_sort | YI Zimei |
| collection | DOAJ |
| description | [Objective:] To investigate the spatial and temporal distribution of p21 senescent cells in periodontal tissue and femur of diabetic mice. [Methods:] We constructed transgenic mice capable of tracing p21 senescent cells, used gel electrophoresis assay to screen 24 homozygous mice, and randomly divided them into control group and diabetic group, with 12 mice in each one. The control group was given a normal diet, while the diabetic group was given a high fat diet for 4 weeks, followed by streptozocin (STZ) injection to induce the diabetic model. Periodontal tissue samples were collected from mice at 4, 8, 12, and 16 weeks respectively. We used the tracer function of p21-3MR mice and real-time quantitative polymerase chain reaction (RT-qPCR) to investigate the spatial and temporal expression changes of p21 senescent cells and periodontal changes in inflammation levels. [Results:] The expression levels of p21 increase over time during the progression of diabetes, and the distribution of p21 senescent cells gradually extends from the gingiva towards the alveolar bone. [Conclusion:] p21 senescent cells are involved in the progression of diabetes and may play a role in the regulation of bone homeostasiss by secretion of senescence-associated secretory phenotype (SASP) factors, but their specific mechanisms require further investigation. |
| format | Article |
| id | doaj-art-931a7df20a594af7a20e4cf33bfa499c |
| institution | Kabale University |
| issn | 1005-4979 |
| language | zho |
| publishDate | 2024-12-01 |
| publisher | Editorial Office of Journal of Oral and Maxillofacial Surgery |
| record_format | Article |
| series | Kouqiang hemian waike zazhi |
| spelling | doaj-art-931a7df20a594af7a20e4cf33bfa499c2025-08-25T06:11:31ZzhoEditorial Office of Journal of Oral and Maxillofacial SurgeryKouqiang hemian waike zazhi1005-49792024-12-0134643544110.12439/kqhm.1005-4979.2024.06.004Spatial and temporal distribution of p21 senescent cells in periodontal tissue of diabetic miceYI Zimei0REN Le1WEI Yu2CHEN Siyi3YAN Jiayu4WU Junhua5Shanghai Engineering Research Center of Tooth Restoration and Regeneration & Tongji Research Institute of Stomatology & Department of Prosthodontics, Shanghai Tongji Stomatological Hospital and Dental School, Tongji University, Shanghai 200072, ChinaShanghai Engineering Research Center of Tooth Restoration and Regeneration & Tongji Research Institute of Stomatology & Department of Prosthodontics, Shanghai Tongji Stomatological Hospital and Dental School, Tongji University, Shanghai 200072, ChinaShanghai Engineering Research Center of Tooth Restoration and Regeneration & Tongji Research Institute of Stomatology & Department of Prosthodontics, Shanghai Tongji Stomatological Hospital and Dental School, Tongji University, Shanghai 200072, ChinaShanghai Engineering Research Center of Tooth Restoration and Regeneration & Tongji Research Institute of Stomatology & Department of Prosthodontics, Shanghai Tongji Stomatological Hospital and Dental School, Tongji University, Shanghai 200072, ChinaShanghai Engineering Research Center of Tooth Restoration and Regeneration & Tongji Research Institute of Stomatology & Department of Prosthodontics, Shanghai Tongji Stomatological Hospital and Dental School, Tongji University, Shanghai 200072, ChinaShanghai Engineering Research Center of Tooth Restoration and Regeneration & Tongji Research Institute of Stomatology & Department of Prosthodontics, Shanghai Tongji Stomatological Hospital and Dental School, Tongji University, Shanghai 200072, China[Objective:] To investigate the spatial and temporal distribution of p21 senescent cells in periodontal tissue and femur of diabetic mice. [Methods:] We constructed transgenic mice capable of tracing p21 senescent cells, used gel electrophoresis assay to screen 24 homozygous mice, and randomly divided them into control group and diabetic group, with 12 mice in each one. The control group was given a normal diet, while the diabetic group was given a high fat diet for 4 weeks, followed by streptozocin (STZ) injection to induce the diabetic model. Periodontal tissue samples were collected from mice at 4, 8, 12, and 16 weeks respectively. We used the tracer function of p21-3MR mice and real-time quantitative polymerase chain reaction (RT-qPCR) to investigate the spatial and temporal expression changes of p21 senescent cells and periodontal changes in inflammation levels. [Results:] The expression levels of p21 increase over time during the progression of diabetes, and the distribution of p21 senescent cells gradually extends from the gingiva towards the alveolar bone. [Conclusion:] p21 senescent cells are involved in the progression of diabetes and may play a role in the regulation of bone homeostasiss by secretion of senescence-associated secretory phenotype (SASP) factors, but their specific mechanisms require further investigation.https://journal06.magtech.org.cn/Jweb_joms/EN/10.12439/kqhm.1005-4979.2024.06.004cellular senescencep21diabetic periodontitis |
| spellingShingle | YI Zimei REN Le WEI Yu CHEN Siyi YAN Jiayu WU Junhua Spatial and temporal distribution of p21 senescent cells in periodontal tissue of diabetic mice Kouqiang hemian waike zazhi cellular senescence p21 diabetic periodontitis |
| title | Spatial and temporal distribution of p21 senescent cells in periodontal tissue of diabetic mice |
| title_full | Spatial and temporal distribution of p21 senescent cells in periodontal tissue of diabetic mice |
| title_fullStr | Spatial and temporal distribution of p21 senescent cells in periodontal tissue of diabetic mice |
| title_full_unstemmed | Spatial and temporal distribution of p21 senescent cells in periodontal tissue of diabetic mice |
| title_short | Spatial and temporal distribution of p21 senescent cells in periodontal tissue of diabetic mice |
| title_sort | spatial and temporal distribution of p21 senescent cells in periodontal tissue of diabetic mice |
| topic | cellular senescence p21 diabetic periodontitis |
| url | https://journal06.magtech.org.cn/Jweb_joms/EN/10.12439/kqhm.1005-4979.2024.06.004 |
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