Clinical and genetic analysis of oral and nasal staphylococcus aureus isolates in dental patients
Abstract Staphylococcus aureus is a common bacterium that sometimes causes various pyogenic diseases. Methicillin resistant S. aureus (MRSA) infections are particularly difficult to treat. Recently, MRSA has been spreading in the community, so it is important to determine the prevalence of MRSA in t...
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Nature Portfolio
2025-04-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-025-93773-0 |
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| author | Tomoki Kawayanagi Miki Kawada-Matsuo Satoru Kusaka Yoshimasa Yasutomi Yujin Suzuki Saki Nishihama Mi Nguyen-Tra Le Yo Sugawara Junzo Hisatsune Shoko Kutsuno Mikari Asakawa Toru Takeshita Ryota Nomura Hideki Shiba Motoyuki Sugai Hitoshi Komatsuzawa |
| author_facet | Tomoki Kawayanagi Miki Kawada-Matsuo Satoru Kusaka Yoshimasa Yasutomi Yujin Suzuki Saki Nishihama Mi Nguyen-Tra Le Yo Sugawara Junzo Hisatsune Shoko Kutsuno Mikari Asakawa Toru Takeshita Ryota Nomura Hideki Shiba Motoyuki Sugai Hitoshi Komatsuzawa |
| author_sort | Tomoki Kawayanagi |
| collection | DOAJ |
| description | Abstract Staphylococcus aureus is a common bacterium that sometimes causes various pyogenic diseases. Methicillin resistant S. aureus (MRSA) infections are particularly difficult to treat. Recently, MRSA has been spreading in the community, so it is important to determine the prevalence of MRSA in the community and to conduct epidemiological studies from genetic and statistical perspectives. In this study, S. aureus/MRSA was isolated from the oral and nasal cavities of 504 dental patients (65 inpatients and 439 outpatients). Sixty-two S. aureus strains and 9 MRSA strains were isolated from the oral cavity, and 112 S. aureus strains and 21 MRSA strains were isolated from the nasal cavity. Multi-locus sequence typing (MLST) analysis showed ST8 was high (18 isolates) among 30 MRSA isolates, whereas among 144 methicillin sensitive isolates, ST15 (25 isolates) and ST8 (20 isolates) were high. Statistical analysis of the patients’ clinical status revealed a correlation between oral S. aureus and denture use. Among the 34 patients from whom S. aureus was isolated from both sites, 25 had the same ST, and 23 showed below 40 single-nucleotide polymorphisms which are considered to be identical strains. Our study revealed various properties of S. aureus/MRSA in the oral and nasal cavities as commensals. |
| format | Article |
| id | doaj-art-9319541a2b494f39ac8d74ddd7b503f6 |
| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-9319541a2b494f39ac8d74ddd7b503f62025-08-20T02:24:29ZengNature PortfolioScientific Reports2045-23222025-04-0115111010.1038/s41598-025-93773-0Clinical and genetic analysis of oral and nasal staphylococcus aureus isolates in dental patientsTomoki Kawayanagi0Miki Kawada-Matsuo1Satoru Kusaka2Yoshimasa Yasutomi3Yujin Suzuki4Saki Nishihama5Mi Nguyen-Tra Le6Yo Sugawara7Junzo Hisatsune8Shoko Kutsuno9Mikari Asakawa10Toru Takeshita11Ryota Nomura12Hideki Shiba13Motoyuki Sugai14Hitoshi Komatsuzawa15Department of Biological Endodontics, Graduate School of Biomedical and Health Sciences, Hiroshima UniversityDepartment of Bacteriology, Graduate School of Biomedical and Health Sciences, Hiroshima UniversityDepartment of Bacteriology, Graduate School of Biomedical and Health Sciences, Hiroshima UniversityDepartment of Bacteriology, Graduate School of Biomedical and Health Sciences, Hiroshima UniversityDepartment of Bacteriology, Graduate School of Biomedical and Health Sciences, Hiroshima UniversityDepartment of Biological Endodontics, Graduate School of Biomedical and Health Sciences, Hiroshima UniversityDepartment of Bacteriology, Graduate School of Biomedical and Health Sciences, Hiroshima UniversityAntimicrobial Resistance Research Center, National Institute of Infectious DiseasesProject Research Center for Nosocomial Infectious Diseases, Hiroshima UniversityAntimicrobial Resistance Research Center, National Institute of Infectious DiseasesSection of Preventive and Public Health Dentistry, Division of Oral Health, Growth and Development, Faculty of Dental Science, Kyushu UniversitySection of Preventive and Public Health Dentistry, Division of Oral Health, Growth and Development, Faculty of Dental Science, Kyushu UniversityDepartment of Pediatric Dentistry, Graduate School of Biomedical and Health Sciences, Hiroshima UniversityDepartment of Biological Endodontics, Graduate School of Biomedical and Health Sciences, Hiroshima UniversityProject Research Center for Nosocomial Infectious Diseases, Hiroshima UniversityDepartment of Bacteriology, Graduate School of Biomedical and Health Sciences, Hiroshima UniversityAbstract Staphylococcus aureus is a common bacterium that sometimes causes various pyogenic diseases. Methicillin resistant S. aureus (MRSA) infections are particularly difficult to treat. Recently, MRSA has been spreading in the community, so it is important to determine the prevalence of MRSA in the community and to conduct epidemiological studies from genetic and statistical perspectives. In this study, S. aureus/MRSA was isolated from the oral and nasal cavities of 504 dental patients (65 inpatients and 439 outpatients). Sixty-two S. aureus strains and 9 MRSA strains were isolated from the oral cavity, and 112 S. aureus strains and 21 MRSA strains were isolated from the nasal cavity. Multi-locus sequence typing (MLST) analysis showed ST8 was high (18 isolates) among 30 MRSA isolates, whereas among 144 methicillin sensitive isolates, ST15 (25 isolates) and ST8 (20 isolates) were high. Statistical analysis of the patients’ clinical status revealed a correlation between oral S. aureus and denture use. Among the 34 patients from whom S. aureus was isolated from both sites, 25 had the same ST, and 23 showed below 40 single-nucleotide polymorphisms which are considered to be identical strains. Our study revealed various properties of S. aureus/MRSA in the oral and nasal cavities as commensals.https://doi.org/10.1038/s41598-025-93773-0 |
| spellingShingle | Tomoki Kawayanagi Miki Kawada-Matsuo Satoru Kusaka Yoshimasa Yasutomi Yujin Suzuki Saki Nishihama Mi Nguyen-Tra Le Yo Sugawara Junzo Hisatsune Shoko Kutsuno Mikari Asakawa Toru Takeshita Ryota Nomura Hideki Shiba Motoyuki Sugai Hitoshi Komatsuzawa Clinical and genetic analysis of oral and nasal staphylococcus aureus isolates in dental patients Scientific Reports |
| title | Clinical and genetic analysis of oral and nasal staphylococcus aureus isolates in dental patients |
| title_full | Clinical and genetic analysis of oral and nasal staphylococcus aureus isolates in dental patients |
| title_fullStr | Clinical and genetic analysis of oral and nasal staphylococcus aureus isolates in dental patients |
| title_full_unstemmed | Clinical and genetic analysis of oral and nasal staphylococcus aureus isolates in dental patients |
| title_short | Clinical and genetic analysis of oral and nasal staphylococcus aureus isolates in dental patients |
| title_sort | clinical and genetic analysis of oral and nasal staphylococcus aureus isolates in dental patients |
| url | https://doi.org/10.1038/s41598-025-93773-0 |
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