Vitronectin regulates focal adhesion turnover and migration of human placenta-derived MSCs under nutrient stress
At sites of tissue damage and wound healing, the mesenchymal stem cells (MSCs) are often challenged by nutrient availability due to blood supply disruption. Thus, it becomes critical to identify novel factors and their mechanism of action in regulating the adhesion and migration of MSCs under nutrie...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-06-01
|
| Series: | European Journal of Cell Biology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0171933525000020 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832573230223720448 |
|---|---|
| author | Srishti Dutta Gupta Nitish Pal Malancha Ta |
| author_facet | Srishti Dutta Gupta Nitish Pal Malancha Ta |
| author_sort | Srishti Dutta Gupta |
| collection | DOAJ |
| description | At sites of tissue damage and wound healing, the mesenchymal stem cells (MSCs) are often challenged by nutrient availability due to blood supply disruption. Thus, it becomes critical to identify novel factors and their mechanism of action in regulating the adhesion and migration of MSCs under nutrient stress condition for successful clinical application. In human placenta-derived MSCs (PL-MSCs), we demonstrated an increase in cell spread area, along with increased adhesion and reduced migration of the cells, when cultured under nutrient stress condition. Correspondingly, an increase in the total number per cell and size of focal adhesions (FAs), together with prominent stress fibers were observed in nutrient-stressed PL-MSCs compared to control PL-MSCs. The FAs were demonstrated to be more stable, exhibiting slower turnover and longer lifespan. Vitronectin (VTN), an ECM glycoprotein, was upregulated under nutrient stress condition. Knockdown of VTN in PL-MSCs led to a significant reduction in the total number per cell and size of FAs, along with their faster turnover and shorter lifespan. Subsequently, a reversal in the cell spread area, adhesion and migration properties of the nutrient-stressed PL-MSCs were noted. Additionally, our findings indicated that VTN, as an upstream regulator, stimulated the phosphorylation of myosin light chain, which possibly promoted the maturation and stability of FAs along with assembly of stress fibers, thereby leading to increased adhesion and reduced migration of the cells. Overall, our study defines a distinct role of VTN as a critical regulator of migration in PL-MSCs under nutrient stress condition. |
| format | Article |
| id | doaj-art-93176b43b4a4465686b970ab385468bc |
| institution | Kabale University |
| issn | 0171-9335 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | European Journal of Cell Biology |
| spelling | doaj-art-93176b43b4a4465686b970ab385468bc2025-02-02T05:26:40ZengElsevierEuropean Journal of Cell Biology0171-93352025-06-011042151477Vitronectin regulates focal adhesion turnover and migration of human placenta-derived MSCs under nutrient stressSrishti Dutta Gupta0Nitish Pal1Malancha Ta2Indian Institute of Science Education and Research, Kolkata (IISER Kolkata), IndiaIndian Institute of Science Education and Research, Kolkata (IISER Kolkata), IndiaCorrespondence to: Department of Biological Sciences, Indian Institute of Science Education and Research (IISER)-Kolkata, Mohanpur Campus, Dist: Nadia, West Bengal 741246, India.; Indian Institute of Science Education and Research, Kolkata (IISER Kolkata), IndiaAt sites of tissue damage and wound healing, the mesenchymal stem cells (MSCs) are often challenged by nutrient availability due to blood supply disruption. Thus, it becomes critical to identify novel factors and their mechanism of action in regulating the adhesion and migration of MSCs under nutrient stress condition for successful clinical application. In human placenta-derived MSCs (PL-MSCs), we demonstrated an increase in cell spread area, along with increased adhesion and reduced migration of the cells, when cultured under nutrient stress condition. Correspondingly, an increase in the total number per cell and size of focal adhesions (FAs), together with prominent stress fibers were observed in nutrient-stressed PL-MSCs compared to control PL-MSCs. The FAs were demonstrated to be more stable, exhibiting slower turnover and longer lifespan. Vitronectin (VTN), an ECM glycoprotein, was upregulated under nutrient stress condition. Knockdown of VTN in PL-MSCs led to a significant reduction in the total number per cell and size of FAs, along with their faster turnover and shorter lifespan. Subsequently, a reversal in the cell spread area, adhesion and migration properties of the nutrient-stressed PL-MSCs were noted. Additionally, our findings indicated that VTN, as an upstream regulator, stimulated the phosphorylation of myosin light chain, which possibly promoted the maturation and stability of FAs along with assembly of stress fibers, thereby leading to increased adhesion and reduced migration of the cells. Overall, our study defines a distinct role of VTN as a critical regulator of migration in PL-MSCs under nutrient stress condition.http://www.sciencedirect.com/science/article/pii/S0171933525000020Placental MSCsVitronectinAdhesionMigrationFocal adhesionsNon-muscle myosin II |
| spellingShingle | Srishti Dutta Gupta Nitish Pal Malancha Ta Vitronectin regulates focal adhesion turnover and migration of human placenta-derived MSCs under nutrient stress European Journal of Cell Biology Placental MSCs Vitronectin Adhesion Migration Focal adhesions Non-muscle myosin II |
| title | Vitronectin regulates focal adhesion turnover and migration of human placenta-derived MSCs under nutrient stress |
| title_full | Vitronectin regulates focal adhesion turnover and migration of human placenta-derived MSCs under nutrient stress |
| title_fullStr | Vitronectin regulates focal adhesion turnover and migration of human placenta-derived MSCs under nutrient stress |
| title_full_unstemmed | Vitronectin regulates focal adhesion turnover and migration of human placenta-derived MSCs under nutrient stress |
| title_short | Vitronectin regulates focal adhesion turnover and migration of human placenta-derived MSCs under nutrient stress |
| title_sort | vitronectin regulates focal adhesion turnover and migration of human placenta derived mscs under nutrient stress |
| topic | Placental MSCs Vitronectin Adhesion Migration Focal adhesions Non-muscle myosin II |
| url | http://www.sciencedirect.com/science/article/pii/S0171933525000020 |
| work_keys_str_mv | AT srishtiduttagupta vitronectinregulatesfocaladhesionturnoverandmigrationofhumanplacentaderivedmscsundernutrientstress AT nitishpal vitronectinregulatesfocaladhesionturnoverandmigrationofhumanplacentaderivedmscsundernutrientstress AT malanchata vitronectinregulatesfocaladhesionturnoverandmigrationofhumanplacentaderivedmscsundernutrientstress |