Microbial dynamics of acute pancreatitis: integrating culture, sequencing, and bile impact on bacterial populations and gaseous metabolites
BackgroundOur study examined the composition of the intestinal microflora in a hospitalized patient with AP symptoms treated several months earlier for diverticulitis. The therapeutic intervention necessitated Hartmann's procedure, culminating in colostomy creation.AimsEmploying a thorough micr...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1544124/full |
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| author | Agnieszka Chmielarczyk Edyta Golińska Anna Tomusiak-Plebanek Natalia Żeber-Lubecka Natalia Żeber-Lubecka Maria Kulecka Maria Kulecka Antoni Szczepanik Katarzyna Jedlińska Krzysztof Mech Konrad Szaciłowski Agata Kuziak Agata Pietrzyk Magdalena Strus |
| author_facet | Agnieszka Chmielarczyk Edyta Golińska Anna Tomusiak-Plebanek Natalia Żeber-Lubecka Natalia Żeber-Lubecka Maria Kulecka Maria Kulecka Antoni Szczepanik Katarzyna Jedlińska Krzysztof Mech Konrad Szaciłowski Agata Kuziak Agata Pietrzyk Magdalena Strus |
| author_sort | Agnieszka Chmielarczyk |
| collection | DOAJ |
| description | BackgroundOur study examined the composition of the intestinal microflora in a hospitalized patient with AP symptoms treated several months earlier for diverticulitis. The therapeutic intervention necessitated Hartmann's procedure, culminating in colostomy creation.AimsEmploying a thorough microbiological analysis we attempted to demonstrate whether the microflora isolated from the peripancreatic fluid exhibited a stronger correlation with the contents of the stoma or with the rectal swab. Additionally, we sought to determine the association between later onset of AP and diverticulitis.MethodsFollowing clinical materials from the patient in the initial phase of AP were collected: rectal swab, colostomy bag contents (in the publication referred to as stoma content/stool) and peripancreatic fluid. Microbiological analysis was performed, including classic culture methodology, NGS techniques, and genotyping methodologies. Furthermore, the effect of bile on the shift in the population of selected bacterial species was examined.ResultsThe NGS technique confirmed greater consistency in bacteria percentage (phyla/family) between stoma content and peripancreatic fluid. In both samples, a clear dominance of the Proteobacteria phyla (over 75%) and the Enterobacteriaceae family was demonstrated. Moreover, NGS verified the presence of the Fusobacteriota phylum and Fusobacteriaceae family only in rectal swabs, which may indicate a link between this type of bacteria and the etiology of diverticulitis. We observed that Escherichia coli 33 isolated from stool exhibited active gaseous metabolite production (mainly hydrogen).ConclusionsThe abundant production of hydrogen may substantially impact enzymatic processes, inducing specific alterations in disulfide bonds and trypsin inactivation. Our investigation alludes to the conceivable active involvement of bile in effecting qualitative and quantitative modifications in the peripancreatic microbiota composition, establishing a correlation between released bile and bacterial generation of gaseous metabolites. |
| format | Article |
| id | doaj-art-9315daf939dc4feeb5e5401579c98dab |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj-art-9315daf939dc4feeb5e5401579c98dab2025-02-12T07:25:30ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-02-011610.3389/fmicb.2025.15441241544124Microbial dynamics of acute pancreatitis: integrating culture, sequencing, and bile impact on bacterial populations and gaseous metabolitesAgnieszka Chmielarczyk0Edyta Golińska1Anna Tomusiak-Plebanek2Natalia Żeber-Lubecka3Natalia Żeber-Lubecka4Maria Kulecka5Maria Kulecka6Antoni Szczepanik7Katarzyna Jedlińska8Krzysztof Mech9Konrad Szaciłowski10Agata Kuziak11Agata Pietrzyk12Magdalena Strus13Department of Microbiology, Faculty of Medicine, Jagiellonian University Medical College, Kraków, PolandDepartment of Microbiology, Faculty of Medicine, Jagiellonian University Medical College, Kraków, PolandDepartment of Microbiology, Faculty of Medicine, Jagiellonian University Medical College, Kraków, PolandDepartment of Gastroenterology, Hepatology and Clinical Oncology, Center of Postgraduate Medical Education, Warsaw, PolandDepartment of Genetics, Maria Sklodowska-Curie National Research Institute of Oncology, Warsaw, PolandDepartment of Gastroenterology, Hepatology and Clinical Oncology, Center of Postgraduate Medical Education, Warsaw, PolandDepartment of Genetics, Maria Sklodowska-Curie National Research Institute of Oncology, Warsaw, PolandClinical Department of General Surgery and Oncology, Narutowicz City Speciality Hospital at Krakow, Krakow, PolandDepartment of Analytical Chemistry and Biochemistry, Faculty of Materials Science and Ceramics, AGH University of Science and Technology of Krakow, Krakow, PolandAcademic Center for Materials and Nanotechnology, AGH University of Krakow, Krakow, PolandAcademic Center for Materials and Nanotechnology, AGH University of Krakow, Krakow, PolandDepartment of Microbiology, Faculty of Medicine, Jagiellonian University Medical College, Kraków, PolandDepartment of Microbiology, Faculty of Medicine, Jagiellonian University Medical College, Kraków, PolandDepartment of Microbiology, Faculty of Medicine, Jagiellonian University Medical College, Kraków, PolandBackgroundOur study examined the composition of the intestinal microflora in a hospitalized patient with AP symptoms treated several months earlier for diverticulitis. The therapeutic intervention necessitated Hartmann's procedure, culminating in colostomy creation.AimsEmploying a thorough microbiological analysis we attempted to demonstrate whether the microflora isolated from the peripancreatic fluid exhibited a stronger correlation with the contents of the stoma or with the rectal swab. Additionally, we sought to determine the association between later onset of AP and diverticulitis.MethodsFollowing clinical materials from the patient in the initial phase of AP were collected: rectal swab, colostomy bag contents (in the publication referred to as stoma content/stool) and peripancreatic fluid. Microbiological analysis was performed, including classic culture methodology, NGS techniques, and genotyping methodologies. Furthermore, the effect of bile on the shift in the population of selected bacterial species was examined.ResultsThe NGS technique confirmed greater consistency in bacteria percentage (phyla/family) between stoma content and peripancreatic fluid. In both samples, a clear dominance of the Proteobacteria phyla (over 75%) and the Enterobacteriaceae family was demonstrated. Moreover, NGS verified the presence of the Fusobacteriota phylum and Fusobacteriaceae family only in rectal swabs, which may indicate a link between this type of bacteria and the etiology of diverticulitis. We observed that Escherichia coli 33 isolated from stool exhibited active gaseous metabolite production (mainly hydrogen).ConclusionsThe abundant production of hydrogen may substantially impact enzymatic processes, inducing specific alterations in disulfide bonds and trypsin inactivation. Our investigation alludes to the conceivable active involvement of bile in effecting qualitative and quantitative modifications in the peripancreatic microbiota composition, establishing a correlation between released bile and bacterial generation of gaseous metabolites.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1544124/fullacute pancreatitismicrobiomemicrobiotabileNGSgaseous metabolites |
| spellingShingle | Agnieszka Chmielarczyk Edyta Golińska Anna Tomusiak-Plebanek Natalia Żeber-Lubecka Natalia Żeber-Lubecka Maria Kulecka Maria Kulecka Antoni Szczepanik Katarzyna Jedlińska Krzysztof Mech Konrad Szaciłowski Agata Kuziak Agata Pietrzyk Magdalena Strus Microbial dynamics of acute pancreatitis: integrating culture, sequencing, and bile impact on bacterial populations and gaseous metabolites Frontiers in Microbiology acute pancreatitis microbiome microbiota bile NGS gaseous metabolites |
| title | Microbial dynamics of acute pancreatitis: integrating culture, sequencing, and bile impact on bacterial populations and gaseous metabolites |
| title_full | Microbial dynamics of acute pancreatitis: integrating culture, sequencing, and bile impact on bacterial populations and gaseous metabolites |
| title_fullStr | Microbial dynamics of acute pancreatitis: integrating culture, sequencing, and bile impact on bacterial populations and gaseous metabolites |
| title_full_unstemmed | Microbial dynamics of acute pancreatitis: integrating culture, sequencing, and bile impact on bacterial populations and gaseous metabolites |
| title_short | Microbial dynamics of acute pancreatitis: integrating culture, sequencing, and bile impact on bacterial populations and gaseous metabolites |
| title_sort | microbial dynamics of acute pancreatitis integrating culture sequencing and bile impact on bacterial populations and gaseous metabolites |
| topic | acute pancreatitis microbiome microbiota bile NGS gaseous metabolites |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1544124/full |
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