Antimicrobial Resistance Genes in Clinical <i>Escherichia coli</i> Strains from Livestock and Poultry in Shandong Province, China During 2015–2020
Antimicrobial resistant (AMR) <i>Escherichia coli</i> (<i>E. coli</i>) isolated from animals may lead to antibiotic treatment failure and economic losses to farmers. The co-existence of antimicrobial resistant genes (ARGs) in the same isolate presents a major challenge for th...
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2025-01-01
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| author | Miaoli Wang Shaopeng Wu Yao Wang Feng Chen Zhangqi Shen Zouran Lan |
| author_facet | Miaoli Wang Shaopeng Wu Yao Wang Feng Chen Zhangqi Shen Zouran Lan |
| author_sort | Miaoli Wang |
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| description | Antimicrobial resistant (AMR) <i>Escherichia coli</i> (<i>E. coli</i>) isolated from animals may lead to antibiotic treatment failure and economic losses to farmers. The co-existence of antimicrobial resistant genes (ARGs) in the same isolate presents a major challenge for the prevention and control of infection in multidrug-resistant (MDR) Gram-negative organisms. There have been a lot of studies on the antibiotic resistance of <i>E. coli</i> in livestock and poultry, but few of them have focused on clinical pathogens. <b>Objective:</b> The aim of this study was to explore the genetic characteristics, co-occurrence, and correlations between ARGs of <i>E. coli</i> isolated from the pathological tissues of livestock and poultry in Shandong Province, East China during 2015–2020. <b>Methods:</b> A total of 158 <i>E. coli</i> strains were collected and subjected to antimicrobial susceptibility testing and sequencing by whole-genome Next Generation Sequencing (NGS). <b>Results:</b> MDR strains accounted for 46.20% of the 158 <i>E. coli</i> strains with the highest resistant rate of ciprofloxacin (71.52%). In addition, strains with <i>bla<sub>NDM-5</sub>/mcr-1.1</i> and <i>mcr-1.1/mcr-3.24</i> were found in chickens, while three strains with <i>Tet(X4)</i> were found in pigs. In addition, the most common serotypes detected were the O serotype (76/158) and H serotype (36/158). Moreover, seventy-one STs were found and the most common STs were ST10 (6.33%), ST155 (6.33%), and ST101 (5.69%). The genetic environment analysis of the phylogroups revealed that <i>E. coli</i> belonging to phylogroup B1, phylogroup A, and phylogroup C constituted 39.87%, 27.85%, and 15.19%, respectively. Through the correlation analysis, <i>mcr</i> genes were observed to have certain relationships with ARGS such as <i>bla<sub>TEM</sub></i>, <i>floR</i>, <i>catA/B</i>, and <i>oqx</i>. <b>Conclusions:</b> This study demonstrates the high prevalence and gene diversity of MDR <i>E. coli</i> isolated from a clinic in Shandong Province, East China. We predicted the transmission risk of animal-borne <i>Tet(X4)</i>-bearing and <i>mcr</i>-harboring <i>E. coli</i> to public health and provided insight into the relationship of co-existence or co-transfer between <i>mcr</i> with ARGS. These relationships present a great challenge for the infection control of MDR Gram-negative organisms. |
| format | Article |
| id | doaj-art-93042826deb24d28abb0bb07ac7601aa |
| institution | Kabale University |
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| spelling | doaj-art-93042826deb24d28abb0bb07ac7601aa2025-01-24T13:18:58ZengMDPI AGAntibiotics2079-63822025-01-011419510.3390/antibiotics14010095Antimicrobial Resistance Genes in Clinical <i>Escherichia coli</i> Strains from Livestock and Poultry in Shandong Province, China During 2015–2020Miaoli Wang0Shaopeng Wu1Yao Wang2Feng Chen3Zhangqi Shen4Zouran Lan5National Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing 100193, ChinaShandong Provincial Key Laboratory of Zoonoses, College of Veterinary Medicine, Shandong Agricultural University, Tai’an 271018, ChinaShandong Center for Animal Disease Control and Prevention, Shandong Centre for Zoonotic Disease Surveillance, Jinan 250100, ChinaShandong Center for Animal Disease Control and Prevention, Shandong Centre for Zoonotic Disease Surveillance, Jinan 250100, ChinaNational Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing 100193, ChinaShandong Center for Animal Disease Control and Prevention, Shandong Centre for Zoonotic Disease Surveillance, Jinan 250100, ChinaAntimicrobial resistant (AMR) <i>Escherichia coli</i> (<i>E. coli</i>) isolated from animals may lead to antibiotic treatment failure and economic losses to farmers. The co-existence of antimicrobial resistant genes (ARGs) in the same isolate presents a major challenge for the prevention and control of infection in multidrug-resistant (MDR) Gram-negative organisms. There have been a lot of studies on the antibiotic resistance of <i>E. coli</i> in livestock and poultry, but few of them have focused on clinical pathogens. <b>Objective:</b> The aim of this study was to explore the genetic characteristics, co-occurrence, and correlations between ARGs of <i>E. coli</i> isolated from the pathological tissues of livestock and poultry in Shandong Province, East China during 2015–2020. <b>Methods:</b> A total of 158 <i>E. coli</i> strains were collected and subjected to antimicrobial susceptibility testing and sequencing by whole-genome Next Generation Sequencing (NGS). <b>Results:</b> MDR strains accounted for 46.20% of the 158 <i>E. coli</i> strains with the highest resistant rate of ciprofloxacin (71.52%). In addition, strains with <i>bla<sub>NDM-5</sub>/mcr-1.1</i> and <i>mcr-1.1/mcr-3.24</i> were found in chickens, while three strains with <i>Tet(X4)</i> were found in pigs. In addition, the most common serotypes detected were the O serotype (76/158) and H serotype (36/158). Moreover, seventy-one STs were found and the most common STs were ST10 (6.33%), ST155 (6.33%), and ST101 (5.69%). The genetic environment analysis of the phylogroups revealed that <i>E. coli</i> belonging to phylogroup B1, phylogroup A, and phylogroup C constituted 39.87%, 27.85%, and 15.19%, respectively. Through the correlation analysis, <i>mcr</i> genes were observed to have certain relationships with ARGS such as <i>bla<sub>TEM</sub></i>, <i>floR</i>, <i>catA/B</i>, and <i>oqx</i>. <b>Conclusions:</b> This study demonstrates the high prevalence and gene diversity of MDR <i>E. coli</i> isolated from a clinic in Shandong Province, East China. We predicted the transmission risk of animal-borne <i>Tet(X4)</i>-bearing and <i>mcr</i>-harboring <i>E. coli</i> to public health and provided insight into the relationship of co-existence or co-transfer between <i>mcr</i> with ARGS. These relationships present a great challenge for the infection control of MDR Gram-negative organisms.https://www.mdpi.com/2079-6382/14/1/95MDR <i>E. coli</i>ARGslivestock and poultrycorrelation analysis |
| spellingShingle | Miaoli Wang Shaopeng Wu Yao Wang Feng Chen Zhangqi Shen Zouran Lan Antimicrobial Resistance Genes in Clinical <i>Escherichia coli</i> Strains from Livestock and Poultry in Shandong Province, China During 2015–2020 Antibiotics MDR <i>E. coli</i> ARGs livestock and poultry correlation analysis |
| title | Antimicrobial Resistance Genes in Clinical <i>Escherichia coli</i> Strains from Livestock and Poultry in Shandong Province, China During 2015–2020 |
| title_full | Antimicrobial Resistance Genes in Clinical <i>Escherichia coli</i> Strains from Livestock and Poultry in Shandong Province, China During 2015–2020 |
| title_fullStr | Antimicrobial Resistance Genes in Clinical <i>Escherichia coli</i> Strains from Livestock and Poultry in Shandong Province, China During 2015–2020 |
| title_full_unstemmed | Antimicrobial Resistance Genes in Clinical <i>Escherichia coli</i> Strains from Livestock and Poultry in Shandong Province, China During 2015–2020 |
| title_short | Antimicrobial Resistance Genes in Clinical <i>Escherichia coli</i> Strains from Livestock and Poultry in Shandong Province, China During 2015–2020 |
| title_sort | antimicrobial resistance genes in clinical i escherichia coli i strains from livestock and poultry in shandong province china during 2015 2020 |
| topic | MDR <i>E. coli</i> ARGs livestock and poultry correlation analysis |
| url | https://www.mdpi.com/2079-6382/14/1/95 |
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