The Role of Dectin-1-Mediated M1 Macrophage Polarization in Cerebral Ischemia-Reperfusion Injury
Introduction. The advances in cerebral ischemia treatment have resulted in a larger proportion of patients get the benefits of rebuilding blood flow to the brain. Then, ischemia-reperfusion injury has emerged as a new essential problem. Dectin-1 plays an important role in cerebral ischemia-reperfusi...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Emergency Medicine International |
| Online Access: | http://dx.doi.org/10.1155/2021/6697271 |
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| author | Chen Zongyun Lin Bixia Ding Fadian Hong Xiaoping Chen Hongbin Deng Yu Liu Qicai Ye Xiaoyi Zeng Kai |
| author_facet | Chen Zongyun Lin Bixia Ding Fadian Hong Xiaoping Chen Hongbin Deng Yu Liu Qicai Ye Xiaoyi Zeng Kai |
| author_sort | Chen Zongyun |
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| description | Introduction. The advances in cerebral ischemia treatment have resulted in a larger proportion of patients get the benefits of rebuilding blood flow to the brain. Then, ischemia-reperfusion injury has emerged as a new essential problem. Dectin-1 plays an important role in cerebral ischemia-reperfusion injury by regulating the function of immune cells. Methods. C57BL/6 was blindly divided into four groups including the sham-operated group and the three different kinds of middle cerebral artery occlusion (MCAO) groups (after 6 hours, 12 hours, and 24 hours after plug removal). The protein expression levels of dectin-1, proapoptosis molecule, and antiapoptosis molecule were measured by using western blot analysis. The brain tissue was analyzed by flow cytometry to detect the M1 macrophage levels. Results. The correlation analysis of dectin-1 and infarct areas showed that there was an obviously positive correlation in between them (R = 0.9603). Dectin-1, cleaved caspase-3, and Bax increased, while antiapoptosis molecule, Bcl-2, decreased at three appropriate time points (after 6 hours, 12 hours, and 24 hours). The level of M1 macrophages in the experimental group increased after ischemia-reperfusion injury compared to the control group. Conclusions. The high expression level of dectin-1 may affect M1 macrophage polarization and brain cell apoptosis in cerebral ischemia-reperfusion injury. |
| format | Article |
| id | doaj-art-93036312aeec482d82fe76c990a49d96 |
| institution | Kabale University |
| issn | 2090-2840 2090-2859 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Emergency Medicine International |
| spelling | doaj-art-93036312aeec482d82fe76c990a49d962025-02-03T06:05:27ZengWileyEmergency Medicine International2090-28402090-28592021-01-01202110.1155/2021/66972716697271The Role of Dectin-1-Mediated M1 Macrophage Polarization in Cerebral Ischemia-Reperfusion InjuryChen Zongyun0Lin Bixia1Ding Fadian2Hong Xiaoping3Chen Hongbin4Deng Yu5Liu Qicai6Ye Xiaoyi7Zeng Kai8Department of Laboratory Medicine, Mindong Hospital Affiliated to Fujian Medical University, Fuan 350000, Fujian Province, ChinaDepartment of Pharmacy, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian Province, ChinaDepartment of Surgery, First Affiliated Hospital, Fujian Medical University, Fuzhou 350000, Fujian Province, ChinaDepartment of Ophthalmology, First Affiliated Hospital, Fujian Medical University, Fuzhou 350000, Fujian Province, ChinaDepartment of Laboratory Medicine, Mindong Hospital Affiliated to Fujian Medical University, Fuan 350000, Fujian Province, ChinaNano Medical Technology Research Institute, Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, School of Pharmacy, Fujian Medical University, Fuzhou 350000, Fujian Province, ChinaCenter for Reproductive Medicine, First Affiliated Hospital, Fujian Medical University, Fuzhou 350000, Fujian Province, ChinaDepartment of Nephrology Medicine, Mindong Hospital Affiliated to Fujian Medical University, Fuan 355000, Fujian Province, ChinaDepartment of Anesthesia, The First Affiliated Hospital of Fujian Medical University, Fuzhou 350000, Fujian Province, ChinaIntroduction. The advances in cerebral ischemia treatment have resulted in a larger proportion of patients get the benefits of rebuilding blood flow to the brain. Then, ischemia-reperfusion injury has emerged as a new essential problem. Dectin-1 plays an important role in cerebral ischemia-reperfusion injury by regulating the function of immune cells. Methods. C57BL/6 was blindly divided into four groups including the sham-operated group and the three different kinds of middle cerebral artery occlusion (MCAO) groups (after 6 hours, 12 hours, and 24 hours after plug removal). The protein expression levels of dectin-1, proapoptosis molecule, and antiapoptosis molecule were measured by using western blot analysis. The brain tissue was analyzed by flow cytometry to detect the M1 macrophage levels. Results. The correlation analysis of dectin-1 and infarct areas showed that there was an obviously positive correlation in between them (R = 0.9603). Dectin-1, cleaved caspase-3, and Bax increased, while antiapoptosis molecule, Bcl-2, decreased at three appropriate time points (after 6 hours, 12 hours, and 24 hours). The level of M1 macrophages in the experimental group increased after ischemia-reperfusion injury compared to the control group. Conclusions. The high expression level of dectin-1 may affect M1 macrophage polarization and brain cell apoptosis in cerebral ischemia-reperfusion injury.http://dx.doi.org/10.1155/2021/6697271 |
| spellingShingle | Chen Zongyun Lin Bixia Ding Fadian Hong Xiaoping Chen Hongbin Deng Yu Liu Qicai Ye Xiaoyi Zeng Kai The Role of Dectin-1-Mediated M1 Macrophage Polarization in Cerebral Ischemia-Reperfusion Injury Emergency Medicine International |
| title | The Role of Dectin-1-Mediated M1 Macrophage Polarization in Cerebral Ischemia-Reperfusion Injury |
| title_full | The Role of Dectin-1-Mediated M1 Macrophage Polarization in Cerebral Ischemia-Reperfusion Injury |
| title_fullStr | The Role of Dectin-1-Mediated M1 Macrophage Polarization in Cerebral Ischemia-Reperfusion Injury |
| title_full_unstemmed | The Role of Dectin-1-Mediated M1 Macrophage Polarization in Cerebral Ischemia-Reperfusion Injury |
| title_short | The Role of Dectin-1-Mediated M1 Macrophage Polarization in Cerebral Ischemia-Reperfusion Injury |
| title_sort | role of dectin 1 mediated m1 macrophage polarization in cerebral ischemia reperfusion injury |
| url | http://dx.doi.org/10.1155/2021/6697271 |
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