Redefining Rhipicephalus sanguineus (sensu lato) species complex in Greece focusing on the mitogenome of Rhipicephalus secundus

Rhipicephalus sanguineus species complex, referred to as R. sanguineus (sensu lato), is distributed globally with some species distributed in specific regions and others spread globally. In Greece, R. sanguineus (s.l.) in dogs, and Rhipicephalus turanicus and Rhipicephalus bursa in livestock, have b...

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Main Authors: Panagiota Ligda, Jan Šlapeta, Anastasios Saratsis, Vaia Kantzoura, Jaisy Chong, Smaragda Sotiraki
Format: Article
Language:English
Published: Elsevier 2025-01-01
Series:Current Research in Parasitology and Vector-Borne Diseases
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Online Access:http://www.sciencedirect.com/science/article/pii/S2667114X24000621
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author Panagiota Ligda
Jan Šlapeta
Anastasios Saratsis
Vaia Kantzoura
Jaisy Chong
Smaragda Sotiraki
author_facet Panagiota Ligda
Jan Šlapeta
Anastasios Saratsis
Vaia Kantzoura
Jaisy Chong
Smaragda Sotiraki
author_sort Panagiota Ligda
collection DOAJ
description Rhipicephalus sanguineus species complex, referred to as R. sanguineus (sensu lato), is distributed globally with some species distributed in specific regions and others spread globally. In Greece, R. sanguineus (s.l.) in dogs, and Rhipicephalus turanicus and Rhipicephalus bursa in livestock, have been repeatedly reported however only based on morphological identification. Recently, there has been a great effort to accurately identify the species belonging to the R. sanguineus species complex, using modern molecular tools and describe their spatial distribution. The aim of this study was to expand the studies on the molecular characterisation of species of the R. sanguineus (s.l.) complex in the Mediterranean region, by molecularly characterising ticks collected from various locations and host species in Greece. Results confirm the presence of Rhipicephalus rutilus, Rhipicephalus secundus, R. bursa, and R. turanicus. The complete mitochondrial genome of R. secundus (approx. 15 kb) from sheep (n = 3) in Greece was sequenced and matched to sequences and morphological data from the type-material from Israel. Additionally, the mitogenome of R. bursa from goats (n = 2) in Greece was sequenced. This study provides a molecular reference for R. secundus, a tick species distributed in the Eastern Mediterranean region.
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spelling doaj-art-9300a9c2a639496bb360b1fbdd8f6b2e2025-08-20T02:36:57ZengElsevierCurrent Research in Parasitology and Vector-Borne Diseases2667-114X2025-01-01710023110.1016/j.crpvbd.2024.100231Redefining Rhipicephalus sanguineus (sensu lato) species complex in Greece focusing on the mitogenome of Rhipicephalus secundusPanagiota Ligda0Jan Šlapeta1Anastasios Saratsis2Vaia Kantzoura3Jaisy Chong4Smaragda Sotiraki5Veterinary Research Institute, Hellenic Agricultural Organization (ELGO) -DIMITRA, Campus ELGO, Thermi, 57001, Thessaloniki, Greece; Corresponding author.Sydney School of Veterinary Science, Faculty of Science, University of Sydney, Sydney, New South Wales, 2006, Australia; The Sydney Infectious Diseases Institute, University of Sydney, New South Wales, 2006, AustraliaVeterinary Research Institute, Hellenic Agricultural Organization (ELGO) -DIMITRA, Campus ELGO, Thermi, 57001, Thessaloniki, GreeceVeterinary Research Institute, Hellenic Agricultural Organization (ELGO) -DIMITRA, Campus ELGO, Thermi, 57001, Thessaloniki, GreeceSydney School of Veterinary Science, Faculty of Science, University of Sydney, Sydney, New South Wales, 2006, AustraliaVeterinary Research Institute, Hellenic Agricultural Organization (ELGO) -DIMITRA, Campus ELGO, Thermi, 57001, Thessaloniki, GreeceRhipicephalus sanguineus species complex, referred to as R. sanguineus (sensu lato), is distributed globally with some species distributed in specific regions and others spread globally. In Greece, R. sanguineus (s.l.) in dogs, and Rhipicephalus turanicus and Rhipicephalus bursa in livestock, have been repeatedly reported however only based on morphological identification. Recently, there has been a great effort to accurately identify the species belonging to the R. sanguineus species complex, using modern molecular tools and describe their spatial distribution. The aim of this study was to expand the studies on the molecular characterisation of species of the R. sanguineus (s.l.) complex in the Mediterranean region, by molecularly characterising ticks collected from various locations and host species in Greece. Results confirm the presence of Rhipicephalus rutilus, Rhipicephalus secundus, R. bursa, and R. turanicus. The complete mitochondrial genome of R. secundus (approx. 15 kb) from sheep (n = 3) in Greece was sequenced and matched to sequences and morphological data from the type-material from Israel. Additionally, the mitogenome of R. bursa from goats (n = 2) in Greece was sequenced. This study provides a molecular reference for R. secundus, a tick species distributed in the Eastern Mediterranean region.http://www.sciencedirect.com/science/article/pii/S2667114X24000621Rhipicephalus sanguineus species complexMolecular characterisationMitogenomeGreece
spellingShingle Panagiota Ligda
Jan Šlapeta
Anastasios Saratsis
Vaia Kantzoura
Jaisy Chong
Smaragda Sotiraki
Redefining Rhipicephalus sanguineus (sensu lato) species complex in Greece focusing on the mitogenome of Rhipicephalus secundus
Current Research in Parasitology and Vector-Borne Diseases
Rhipicephalus sanguineus species complex
Molecular characterisation
Mitogenome
Greece
title Redefining Rhipicephalus sanguineus (sensu lato) species complex in Greece focusing on the mitogenome of Rhipicephalus secundus
title_full Redefining Rhipicephalus sanguineus (sensu lato) species complex in Greece focusing on the mitogenome of Rhipicephalus secundus
title_fullStr Redefining Rhipicephalus sanguineus (sensu lato) species complex in Greece focusing on the mitogenome of Rhipicephalus secundus
title_full_unstemmed Redefining Rhipicephalus sanguineus (sensu lato) species complex in Greece focusing on the mitogenome of Rhipicephalus secundus
title_short Redefining Rhipicephalus sanguineus (sensu lato) species complex in Greece focusing on the mitogenome of Rhipicephalus secundus
title_sort redefining rhipicephalus sanguineus sensu lato species complex in greece focusing on the mitogenome of rhipicephalus secundus
topic Rhipicephalus sanguineus species complex
Molecular characterisation
Mitogenome
Greece
url http://www.sciencedirect.com/science/article/pii/S2667114X24000621
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