Impact of isothiazolinone biocides on gonadal steroid production: Focus on 3β-hydroxysteroid dehydrogenase inhibition
Isothiazolinone biocides have been widely used as disinfectants during the COVID-19 pandemic. However, their effects on gonadal steroidogenesis remain unclear. This study investigated the endocrine-disrupting potential of isothiazolinone biocides, focusing on their inhibition of gonadal 3β-hydroxyst...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-06-01
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| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651325005627 |
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| Summary: | Isothiazolinone biocides have been widely used as disinfectants during the COVID-19 pandemic. However, their effects on gonadal steroidogenesis remain unclear. This study investigated the endocrine-disrupting potential of isothiazolinone biocides, focusing on their inhibition of gonadal 3β-hydroxysteroid dehydrogenases (3β-HSDs). Enzymatic assays, molecular docking, and 3D-QSAR analyses evaluated the impact of seven isothiazolinones on human KGN cell 3β-HSD2 (h3β-HSD2) and rat testicular 3β-HSD1 (r3β-HSD1). Dichlorooctylisothiazolinone, butylbenzo[d]isothiazol-3(2 H)-one, octylisothiazolinone, and benzisothiazolinone inhibited h3β-HSD2 with IC50 values of 4.02, 15.35, 12.12, and 30.55 μM, respectively. These compounds also inhibited r3β-HSD1 with IC50 values of 1.67, 16.51, 13.25, and 29.28 μM, respectively. Whereas, methylchloroisothiazolinone selectively inhibited r3β-HSD1 with IC50 value of 40.11 μM. The compounds acted as mixed/noncompetitive inhibitors, targeting the NAD+ binding pocket. Inhibitory potency correlated with compound hydrophobicity, heavy atom count, and molecular weight. A 3D-QSAR pharmacophore model achieved high predictive accuracy (R2 = 0.98) for h3β-HSD2 inhibition. These biocides suppressed progesterone production in human KGN granulosa tumor cells. The binding free energies were ranged from −6.52 to −7.19 kcal/mol for h3β-HSD2 and −6.0 to −7.04 kcal/mol for r3β-HSD1. The results underscore the endocrine-disrupting capabilities of isothiazolinones via inhibition of critical steroidogenic enzymes, offering insights into their structure-activity relationships and potential side effects. |
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| ISSN: | 0147-6513 |