Effects of Fermented Liquid Feed with Compound Probiotics on Growth Performance, Meat Quality, and Fecal Microbiota of Growing Pigs

Feed fermentation enhances feed nutrition and animal health, but its impact on the gut microbiota of young pigs remains unclear. This study aimed to evaluate the effects of a probiotic fermented feed, which includes <i>Lactobacillus plantarum</i>, <i>Pediococcus pentosaceus</i&g...

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Main Authors: Mengting Ji, Xiaoyin Rong, Yifan Wu, Haonan Li, Xiaolei Zhao, Yan Zhao, Xiaohong Guo, Guoqing Cao, Yang Yang, Bugao Li
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:Animals
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Online Access:https://www.mdpi.com/2076-2615/15/5/733
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author Mengting Ji
Xiaoyin Rong
Yifan Wu
Haonan Li
Xiaolei Zhao
Yan Zhao
Xiaohong Guo
Guoqing Cao
Yang Yang
Bugao Li
author_facet Mengting Ji
Xiaoyin Rong
Yifan Wu
Haonan Li
Xiaolei Zhao
Yan Zhao
Xiaohong Guo
Guoqing Cao
Yang Yang
Bugao Li
author_sort Mengting Ji
collection DOAJ
description Feed fermentation enhances feed nutrition and animal health, but its impact on the gut microbiota of young pigs remains unclear. This study aimed to evaluate the effects of a probiotic fermented feed, which includes <i>Lactobacillus plantarum</i>, <i>Pediococcus pentosaceus</i>, <i>Bacillus subtilis</i>, and <i>Bacillus coagulans</i>, on the growth performance, meat quality, and intestinal microbiota of growing pigs. We randomly assigned 24 Duroc × Landrace × Landrace pigs to two groups: a control (Ctrl) group and the fermented liquid feed (FLF) group, with three replicate pens per group and four pigs per pen. Results indicated that the FLF group experienced a significant decrease in anti-nutritional factors like α-conglycinin and β-conglycinin. In addition, the average daily gain of pigs in the FLF group increased significantly, while the feed conversion ratio and shear force decreased. HE staining showed that the FLF group had notably enhanced villus height in the jejunum and ileum. 16S rRNA sequencing revealed a marked increase in the relative abundance of certain microbes in the FLF group, which were predominantly associated with carbohydrate and amino acid metabolism. These results indicated that compound probiotic FLF can elevate feed quality, enhance the growth performance of growing pigs, and ameliorate the structure of the gut microbiota.
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spelling doaj-art-92cfc83eae9e4b3bbeb258f03171f00d2025-08-20T02:04:34ZengMDPI AGAnimals2076-26152025-03-0115573310.3390/ani15050733Effects of Fermented Liquid Feed with Compound Probiotics on Growth Performance, Meat Quality, and Fecal Microbiota of Growing PigsMengting Ji0Xiaoyin Rong1Yifan Wu2Haonan Li3Xiaolei Zhao4Yan Zhao5Xiaohong Guo6Guoqing Cao7Yang Yang8Bugao Li9Key Laboratory of Animal Science, College of Animal Science, Shanxi Agricultural University, No.1, Mingxian South Road, Taigu, Jinzhong 030801, ChinaKey Laboratory of Animal Science, College of Animal Science, Shanxi Agricultural University, No.1, Mingxian South Road, Taigu, Jinzhong 030801, ChinaKey Laboratory of Animal Science, College of Animal Science, Shanxi Agricultural University, No.1, Mingxian South Road, Taigu, Jinzhong 030801, ChinaKey Laboratory of Animal Science, College of Animal Science, Shanxi Agricultural University, No.1, Mingxian South Road, Taigu, Jinzhong 030801, ChinaKey Laboratory of Animal Science, College of Animal Science, Shanxi Agricultural University, No.1, Mingxian South Road, Taigu, Jinzhong 030801, ChinaKey Laboratory of Animal Science, College of Animal Science, Shanxi Agricultural University, No.1, Mingxian South Road, Taigu, Jinzhong 030801, ChinaKey Laboratory of Animal Science, College of Animal Science, Shanxi Agricultural University, No.1, Mingxian South Road, Taigu, Jinzhong 030801, ChinaKey Laboratory of Animal Science, College of Animal Science, Shanxi Agricultural University, No.1, Mingxian South Road, Taigu, Jinzhong 030801, ChinaKey Laboratory of Animal Science, College of Animal Science, Shanxi Agricultural University, No.1, Mingxian South Road, Taigu, Jinzhong 030801, ChinaKey Laboratory of Animal Science, College of Animal Science, Shanxi Agricultural University, No.1, Mingxian South Road, Taigu, Jinzhong 030801, ChinaFeed fermentation enhances feed nutrition and animal health, but its impact on the gut microbiota of young pigs remains unclear. This study aimed to evaluate the effects of a probiotic fermented feed, which includes <i>Lactobacillus plantarum</i>, <i>Pediococcus pentosaceus</i>, <i>Bacillus subtilis</i>, and <i>Bacillus coagulans</i>, on the growth performance, meat quality, and intestinal microbiota of growing pigs. We randomly assigned 24 Duroc × Landrace × Landrace pigs to two groups: a control (Ctrl) group and the fermented liquid feed (FLF) group, with three replicate pens per group and four pigs per pen. Results indicated that the FLF group experienced a significant decrease in anti-nutritional factors like α-conglycinin and β-conglycinin. In addition, the average daily gain of pigs in the FLF group increased significantly, while the feed conversion ratio and shear force decreased. HE staining showed that the FLF group had notably enhanced villus height in the jejunum and ileum. 16S rRNA sequencing revealed a marked increase in the relative abundance of certain microbes in the FLF group, which were predominantly associated with carbohydrate and amino acid metabolism. These results indicated that compound probiotic FLF can elevate feed quality, enhance the growth performance of growing pigs, and ameliorate the structure of the gut microbiota.https://www.mdpi.com/2076-2615/15/5/733fermented liquid feedgrowing pigsmeat qualitygrowth performancegut microorganism
spellingShingle Mengting Ji
Xiaoyin Rong
Yifan Wu
Haonan Li
Xiaolei Zhao
Yan Zhao
Xiaohong Guo
Guoqing Cao
Yang Yang
Bugao Li
Effects of Fermented Liquid Feed with Compound Probiotics on Growth Performance, Meat Quality, and Fecal Microbiota of Growing Pigs
Animals
fermented liquid feed
growing pigs
meat quality
growth performance
gut microorganism
title Effects of Fermented Liquid Feed with Compound Probiotics on Growth Performance, Meat Quality, and Fecal Microbiota of Growing Pigs
title_full Effects of Fermented Liquid Feed with Compound Probiotics on Growth Performance, Meat Quality, and Fecal Microbiota of Growing Pigs
title_fullStr Effects of Fermented Liquid Feed with Compound Probiotics on Growth Performance, Meat Quality, and Fecal Microbiota of Growing Pigs
title_full_unstemmed Effects of Fermented Liquid Feed with Compound Probiotics on Growth Performance, Meat Quality, and Fecal Microbiota of Growing Pigs
title_short Effects of Fermented Liquid Feed with Compound Probiotics on Growth Performance, Meat Quality, and Fecal Microbiota of Growing Pigs
title_sort effects of fermented liquid feed with compound probiotics on growth performance meat quality and fecal microbiota of growing pigs
topic fermented liquid feed
growing pigs
meat quality
growth performance
gut microorganism
url https://www.mdpi.com/2076-2615/15/5/733
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