Assessment of Damage to Nucleic Acids and Repair Machinery in Salmonella typhimurium Exposed to Chlorine
Water disinfection is usually evaluated using mandatory methods based on cell culturability. However, such methods do not consider the potential of cells to recover, which should also be kept as low as possible. In this paper, we hypothesized that a successful disinfection is achieved only when the...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2009-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2009/201868 |
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| author | M. H. Phe M. Hajj Chehade H. Guilloteau C. Merlin J. C. Block |
| author_facet | M. H. Phe M. Hajj Chehade H. Guilloteau C. Merlin J. C. Block |
| author_sort | M. H. Phe |
| collection | DOAJ |
| description | Water disinfection is usually evaluated using mandatory methods based on cell culturability. However, such methods do not consider the potential of cells to recover, which should also be kept as low as possible. In this paper, we hypothesized that a successful disinfection is achieved only when the applied chlorine leads to both intracellular nucleic acid damage and strong alterations of the DNA repair machinery. Monitoring the SOS system responsiveness with a umuC'-‘lacZ reporter fusion, we found that the expression of this important cellular machinery was altered after the beginning of membrane permeabilization but prior to the total decline of both the cell culturability and the nucleic acid integrity as revealed by Sybr-II staining. Rapid measurement of such nucleic acid alterations by fluorochrome-based staining could be used as an alternative method for assessing the effectiveness of disinfection with chlorine. |
| format | Article |
| id | doaj-art-92bd29faf61747cf86a569de4ace2739 |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-92bd29faf61747cf86a569de4ace27392025-02-03T01:28:55ZengWileyInternational Journal of Microbiology1687-918X1687-91982009-01-01200910.1155/2009/201868201868Assessment of Damage to Nucleic Acids and Repair Machinery in Salmonella typhimurium Exposed to ChlorineM. H. Phe0M. Hajj Chehade1H. Guilloteau2C. Merlin3J. C. Block4Laboratoire de Chimie Physique et Microbiologie pour l'Environnement (LCPME), UMR 7564 CNRS, Nancy-Université, 405 rue de Vandoeuvre, 54600 Villers-lès-Nancy, FranceLaboratoire de Chimie Physique et Microbiologie pour l'Environnement (LCPME), UMR 7564 CNRS, Nancy-Université, 405 rue de Vandoeuvre, 54600 Villers-lès-Nancy, FranceLaboratoire de Chimie Physique et Microbiologie pour l'Environnement (LCPME), UMR 7564 CNRS, Nancy-Université, 405 rue de Vandoeuvre, 54600 Villers-lès-Nancy, FranceLaboratoire de Chimie Physique et Microbiologie pour l'Environnement (LCPME), UMR 7564 CNRS, Nancy-Université, 405 rue de Vandoeuvre, 54600 Villers-lès-Nancy, FranceLaboratoire de Chimie Physique et Microbiologie pour l'Environnement (LCPME), UMR 7564 CNRS, Nancy-Université, 405 rue de Vandoeuvre, 54600 Villers-lès-Nancy, FranceWater disinfection is usually evaluated using mandatory methods based on cell culturability. However, such methods do not consider the potential of cells to recover, which should also be kept as low as possible. In this paper, we hypothesized that a successful disinfection is achieved only when the applied chlorine leads to both intracellular nucleic acid damage and strong alterations of the DNA repair machinery. Monitoring the SOS system responsiveness with a umuC'-‘lacZ reporter fusion, we found that the expression of this important cellular machinery was altered after the beginning of membrane permeabilization but prior to the total decline of both the cell culturability and the nucleic acid integrity as revealed by Sybr-II staining. Rapid measurement of such nucleic acid alterations by fluorochrome-based staining could be used as an alternative method for assessing the effectiveness of disinfection with chlorine.http://dx.doi.org/10.1155/2009/201868 |
| spellingShingle | M. H. Phe M. Hajj Chehade H. Guilloteau C. Merlin J. C. Block Assessment of Damage to Nucleic Acids and Repair Machinery in Salmonella typhimurium Exposed to Chlorine International Journal of Microbiology |
| title | Assessment of Damage to Nucleic Acids and Repair Machinery in Salmonella typhimurium Exposed to Chlorine |
| title_full | Assessment of Damage to Nucleic Acids and Repair Machinery in Salmonella typhimurium Exposed to Chlorine |
| title_fullStr | Assessment of Damage to Nucleic Acids and Repair Machinery in Salmonella typhimurium Exposed to Chlorine |
| title_full_unstemmed | Assessment of Damage to Nucleic Acids and Repair Machinery in Salmonella typhimurium Exposed to Chlorine |
| title_short | Assessment of Damage to Nucleic Acids and Repair Machinery in Salmonella typhimurium Exposed to Chlorine |
| title_sort | assessment of damage to nucleic acids and repair machinery in salmonella typhimurium exposed to chlorine |
| url | http://dx.doi.org/10.1155/2009/201868 |
| work_keys_str_mv | AT mhphe assessmentofdamagetonucleicacidsandrepairmachineryinsalmonellatyphimuriumexposedtochlorine AT mhajjchehade assessmentofdamagetonucleicacidsandrepairmachineryinsalmonellatyphimuriumexposedtochlorine AT hguilloteau assessmentofdamagetonucleicacidsandrepairmachineryinsalmonellatyphimuriumexposedtochlorine AT cmerlin assessmentofdamagetonucleicacidsandrepairmachineryinsalmonellatyphimuriumexposedtochlorine AT jcblock assessmentofdamagetonucleicacidsandrepairmachineryinsalmonellatyphimuriumexposedtochlorine |