Application of Real-Time PCR Syndromic Panel on Lower Respiratory Tract Samples: Potential Use for Antimicrobial De-Escalation
Molecular methods allow for a rapid identification of the main causative agents of pneumonia along with the most frequent resistance genes. Prolonged broad-spectrum antibiotic therapy without microbiological evidence of infection drives antimicrobial resistance. We evaluated if the result provided b...
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MDPI AG
2025-07-01
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| author | Christian Leli Paolo Bottino Lidia Ferrara Luigi Di Matteo Franca Gotta Daria Vay Elisa Cornaglia Mattia Zenato Chiara Di Bella Elisabetta Scomparin Cesare Bolla Valeria Bonato Laura Savi Annalisa Roveta Antonio Maconi Andrea Rocchetti |
| author_facet | Christian Leli Paolo Bottino Lidia Ferrara Luigi Di Matteo Franca Gotta Daria Vay Elisa Cornaglia Mattia Zenato Chiara Di Bella Elisabetta Scomparin Cesare Bolla Valeria Bonato Laura Savi Annalisa Roveta Antonio Maconi Andrea Rocchetti |
| author_sort | Christian Leli |
| collection | DOAJ |
| description | Molecular methods allow for a rapid identification of the main causative agents of pneumonia along with the most frequent resistance genes. Prolonged broad-spectrum antibiotic therapy without microbiological evidence of infection drives antimicrobial resistance. We evaluated if the result provided by the molecular method is helpful for antimicrobial de-escalation. All respiratory samples collected and directly processed via Real-Time PCR from patients with suspected pneumonia, of whom clinical data were available, were included in this study. In 82 patients out of a total of 174 (47.1%), antimicrobial therapy was modified after the molecular test, and in 28/82 (34.1%), antimicrobial de-escalation was carried out. Among the 92 patients in whom therapy was not modified, 33 (35.9%) were did not receive any antimicrobial therapy before the molecular test and no antibiotics were prescribed after the test. Therefore, in 61 (28 + 33) out of the 174 (35%) patients, unnecessary antimicrobials were discontinued or avoided. The syndromic panel used at our institution can be of help in better choosing when empiric antibiotic de-escalation therapy could be feasible. |
| format | Article |
| id | doaj-art-92b9937f1beb4be6ba869f930294f9b6 |
| institution | DOAJ |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-92b9937f1beb4be6ba869f930294f9b62025-08-20T03:07:58ZengMDPI AGMicroorganisms2076-26072025-07-01137167810.3390/microorganisms13071678Application of Real-Time PCR Syndromic Panel on Lower Respiratory Tract Samples: Potential Use for Antimicrobial De-EscalationChristian Leli0Paolo Bottino1Lidia Ferrara2Luigi Di Matteo3Franca Gotta4Daria Vay5Elisa Cornaglia6Mattia Zenato7Chiara Di Bella8Elisabetta Scomparin9Cesare Bolla10Valeria Bonato11Laura Savi12Annalisa Roveta13Antonio Maconi14Andrea Rocchetti15Microbiology and Virology Laboratory, University Hospital “SS Antonio e Biagio e C. Arrigo”, Via Venezia 16, 15121 Alessandria, ItalyMicrobiology and Virology Laboratory, University Hospital “SS Antonio e Biagio e C. Arrigo”, Via Venezia 16, 15121 Alessandria, ItalyMicrobiology and Virology Laboratory, University Hospital “SS Antonio e Biagio e C. Arrigo”, Via Venezia 16, 15121 Alessandria, ItalyMicrobiology and Virology Laboratory, University Hospital “SS Antonio e Biagio e C. Arrigo”, Via Venezia 16, 15121 Alessandria, ItalyMicrobiology and Virology Laboratory, University Hospital “SS Antonio e Biagio e C. Arrigo”, Via Venezia 16, 15121 Alessandria, ItalyMicrobiology and Virology Laboratory, University Hospital “SS Antonio e Biagio e C. Arrigo”, Via Venezia 16, 15121 Alessandria, ItalyMicrobiology and Virology Laboratory, University Hospital “SS Antonio e Biagio e C. Arrigo”, Via Venezia 16, 15121 Alessandria, ItalyDepartment of Science and Technological Innovation (DISIT), University of Eastern Piedmont, Viale Teresa Michel 12 11, 15121 Alessandria, ItalyDepartment of Science and Technological Innovation (DISIT), University of Eastern Piedmont, Viale Teresa Michel 12 11, 15121 Alessandria, ItalyMicrobiology and Virology Laboratory, University Hospital “SS Antonio e Biagio e C. Arrigo”, Via Venezia 16, 15121 Alessandria, ItalyInfection and Prevention Control Unit and Antimicrobial Stewardship, University Hospital “SS Antonio e Biagio e C. Arrigo”, Via Venezia 16, 15121 Alessandria, ItalyIntensive Care Unit, University Hospital “SS Antonio e Biagio e C. Arrigo”, Via Venezia 16, 15121 Alessandria, ItalyHospital Pharmacy Management of Devices and Drugs, University Hospital “SS Antonio e Biagio e C. Arrigo”, Via Venezia 16, 15121 Alessandria, ItalyResearch and Innovation Department (DAIRI), University Hospital “SS. Antonio e Biagio e C. Arrigo”, Via Venezia 16, 15121 Alessandria, ItalyResearch and Innovation Department (DAIRI), University Hospital “SS. Antonio e Biagio e C. Arrigo”, Via Venezia 16, 15121 Alessandria, ItalyMicrobiology and Virology Laboratory, University Hospital “SS Antonio e Biagio e C. Arrigo”, Via Venezia 16, 15121 Alessandria, ItalyMolecular methods allow for a rapid identification of the main causative agents of pneumonia along with the most frequent resistance genes. Prolonged broad-spectrum antibiotic therapy without microbiological evidence of infection drives antimicrobial resistance. We evaluated if the result provided by the molecular method is helpful for antimicrobial de-escalation. All respiratory samples collected and directly processed via Real-Time PCR from patients with suspected pneumonia, of whom clinical data were available, were included in this study. In 82 patients out of a total of 174 (47.1%), antimicrobial therapy was modified after the molecular test, and in 28/82 (34.1%), antimicrobial de-escalation was carried out. Among the 92 patients in whom therapy was not modified, 33 (35.9%) were did not receive any antimicrobial therapy before the molecular test and no antibiotics were prescribed after the test. Therefore, in 61 (28 + 33) out of the 174 (35%) patients, unnecessary antimicrobials were discontinued or avoided. The syndromic panel used at our institution can be of help in better choosing when empiric antibiotic de-escalation therapy could be feasible.https://www.mdpi.com/2076-2607/13/7/1678real-time PCRmicrobiologypneumoniaantimicrobial stewardshipdiagnostics |
| spellingShingle | Christian Leli Paolo Bottino Lidia Ferrara Luigi Di Matteo Franca Gotta Daria Vay Elisa Cornaglia Mattia Zenato Chiara Di Bella Elisabetta Scomparin Cesare Bolla Valeria Bonato Laura Savi Annalisa Roveta Antonio Maconi Andrea Rocchetti Application of Real-Time PCR Syndromic Panel on Lower Respiratory Tract Samples: Potential Use for Antimicrobial De-Escalation Microorganisms real-time PCR microbiology pneumonia antimicrobial stewardship diagnostics |
| title | Application of Real-Time PCR Syndromic Panel on Lower Respiratory Tract Samples: Potential Use for Antimicrobial De-Escalation |
| title_full | Application of Real-Time PCR Syndromic Panel on Lower Respiratory Tract Samples: Potential Use for Antimicrobial De-Escalation |
| title_fullStr | Application of Real-Time PCR Syndromic Panel on Lower Respiratory Tract Samples: Potential Use for Antimicrobial De-Escalation |
| title_full_unstemmed | Application of Real-Time PCR Syndromic Panel on Lower Respiratory Tract Samples: Potential Use for Antimicrobial De-Escalation |
| title_short | Application of Real-Time PCR Syndromic Panel on Lower Respiratory Tract Samples: Potential Use for Antimicrobial De-Escalation |
| title_sort | application of real time pcr syndromic panel on lower respiratory tract samples potential use for antimicrobial de escalation |
| topic | real-time PCR microbiology pneumonia antimicrobial stewardship diagnostics |
| url | https://www.mdpi.com/2076-2607/13/7/1678 |
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