Exploring oxysterols and protein carbonylation in cervicovaginal secretions as biomarkers for cervical cancer development
Cervical cancer, a major global health issue and the fourth most common cancer among women, is strongly linked to Human Papillomavirus (HPV) infection. Emerging evidence indicates that oxidative stress plays a critical role in the carcinogenesis of cervical tissue. This study investigates the relati...
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Elsevier
2024-12-01
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| Series: | Advances in Redox Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667137924000183 |
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| author | Busra Kose Serkan Erkanlı Alper Koçak Coskun Guzel Theo Luider Irundika H.K. Dias Ahmet Tarik Baykal |
| author_facet | Busra Kose Serkan Erkanlı Alper Koçak Coskun Guzel Theo Luider Irundika H.K. Dias Ahmet Tarik Baykal |
| author_sort | Busra Kose |
| collection | DOAJ |
| description | Cervical cancer, a major global health issue and the fourth most common cancer among women, is strongly linked to Human Papillomavirus (HPV) infection. Emerging evidence indicates that oxidative stress plays a critical role in the carcinogenesis of cervical tissue. This study investigates the relationship between oxidative stress markers—specifically oxysterols, lipid oxidation, and protein carbonylation—and the progression of cervical neoplasia.Oxysterols, which are elevated in various inflammatory diseases and cancers, were measured in cervicovaginal fluid samples using LC-MS/MS. The targeted oxysterols included 27-hydroxycholesterol (27-OHC), 7β-hydroxycholesterol (7β-OHC), 7-ketocholesterol (7-KC), and 7α,27-dihydroxycholesterol (7α,27-diOHC). Among these, 7α,27-dihydroxycholesterol was significantly increased in correlation with the severity of neoplastic stages. In parallel, protein carbonylation, an indicator of cellular oxidative stress, was assessed. Results revealed higher levels of protein carbonylation in neoplastic samples compared to non-neoplastic controls. These modifications were further analysed through redox proteomics to identify the specific proteins affected.The study demonstrates that elevated lipid oxidation and protein carbonylation in cervicovaginal secretions are linked to the development and progression of cervical cancer. Identifying these biomarkers may improve screening strategies, enabling the identification of individuals at increased risk for cervical neoplasia and guiding timely interventions. |
| format | Article |
| id | doaj-art-92b5fd7e8b704c55a4dbccf35de247bb |
| institution | OA Journals |
| issn | 2667-1379 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Advances in Redox Research |
| spelling | doaj-art-92b5fd7e8b704c55a4dbccf35de247bb2025-08-20T01:59:09ZengElsevierAdvances in Redox Research2667-13792024-12-011310011110.1016/j.arres.2024.100111Exploring oxysterols and protein carbonylation in cervicovaginal secretions as biomarkers for cervical cancer developmentBusra Kose0Serkan Erkanlı1Alper Koçak2Coskun Guzel3Theo Luider4Irundika H.K. Dias5Ahmet Tarik Baykal6Department of Biochemistry and Molecular Biology, Institute of Health Sciences, Acibadem Mehmet Ali Aydinlar University, Istanbul, TurkeyDepartment of Obstetrics and Gynecology, Faculty of Medicine, Acibadem Mehmet Ali Aydinlar University, Istanbul, Turkey; Department of Gynecological Oncology, Acibadem Altunizade Hospital, Istanbul, TurkeyDepartment of Gynecological Oncology, Acibadem Altunizade Hospital, Istanbul, TurkeyClinical & Cancer Proteomics, Department of Neurology, Erasmus MC, Rotterdam, The NetherlandsClinical & Cancer Proteomics, Department of Neurology, Erasmus MC, Rotterdam, The NetherlandsAston Medical School, College of Health and Life Sciences, Aston University, Birmingham, United Kingdom; Corresponding author.Department of Medical Biochemistry, Faculty of Medicine, Acibadem Mehmet Ali Aydinlar University, Istanbul, TurkeyCervical cancer, a major global health issue and the fourth most common cancer among women, is strongly linked to Human Papillomavirus (HPV) infection. Emerging evidence indicates that oxidative stress plays a critical role in the carcinogenesis of cervical tissue. This study investigates the relationship between oxidative stress markers—specifically oxysterols, lipid oxidation, and protein carbonylation—and the progression of cervical neoplasia.Oxysterols, which are elevated in various inflammatory diseases and cancers, were measured in cervicovaginal fluid samples using LC-MS/MS. The targeted oxysterols included 27-hydroxycholesterol (27-OHC), 7β-hydroxycholesterol (7β-OHC), 7-ketocholesterol (7-KC), and 7α,27-dihydroxycholesterol (7α,27-diOHC). Among these, 7α,27-dihydroxycholesterol was significantly increased in correlation with the severity of neoplastic stages. In parallel, protein carbonylation, an indicator of cellular oxidative stress, was assessed. Results revealed higher levels of protein carbonylation in neoplastic samples compared to non-neoplastic controls. These modifications were further analysed through redox proteomics to identify the specific proteins affected.The study demonstrates that elevated lipid oxidation and protein carbonylation in cervicovaginal secretions are linked to the development and progression of cervical cancer. Identifying these biomarkers may improve screening strategies, enabling the identification of individuals at increased risk for cervical neoplasia and guiding timely interventions.http://www.sciencedirect.com/science/article/pii/S2667137924000183Cervical cancerOxidative stressHydroxycholesterolCervicovaginal fluidProtein carbonylation |
| spellingShingle | Busra Kose Serkan Erkanlı Alper Koçak Coskun Guzel Theo Luider Irundika H.K. Dias Ahmet Tarik Baykal Exploring oxysterols and protein carbonylation in cervicovaginal secretions as biomarkers for cervical cancer development Advances in Redox Research Cervical cancer Oxidative stress Hydroxycholesterol Cervicovaginal fluid Protein carbonylation |
| title | Exploring oxysterols and protein carbonylation in cervicovaginal secretions as biomarkers for cervical cancer development |
| title_full | Exploring oxysterols and protein carbonylation in cervicovaginal secretions as biomarkers for cervical cancer development |
| title_fullStr | Exploring oxysterols and protein carbonylation in cervicovaginal secretions as biomarkers for cervical cancer development |
| title_full_unstemmed | Exploring oxysterols and protein carbonylation in cervicovaginal secretions as biomarkers for cervical cancer development |
| title_short | Exploring oxysterols and protein carbonylation in cervicovaginal secretions as biomarkers for cervical cancer development |
| title_sort | exploring oxysterols and protein carbonylation in cervicovaginal secretions as biomarkers for cervical cancer development |
| topic | Cervical cancer Oxidative stress Hydroxycholesterol Cervicovaginal fluid Protein carbonylation |
| url | http://www.sciencedirect.com/science/article/pii/S2667137924000183 |
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