An osteoblast-derived proteinase controls tumor cell survival via TGF-beta activation in the bone microenvironment.
<h4>Background</h4>Breast to bone metastases frequently induce a "vicious cycle" in which osteoclast mediated bone resorption and proteolysis results in the release of bone matrix sequestered factors that drive tumor growth. While osteoclasts express numerous proteinases, analy...
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0029862&type=printable |
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| author | Sophie Thiolloy James R Edwards Barbara Fingleton Daniel B Rifkin Lynn M Matrisian Conor C Lynch |
| author_facet | Sophie Thiolloy James R Edwards Barbara Fingleton Daniel B Rifkin Lynn M Matrisian Conor C Lynch |
| author_sort | Sophie Thiolloy |
| collection | DOAJ |
| description | <h4>Background</h4>Breast to bone metastases frequently induce a "vicious cycle" in which osteoclast mediated bone resorption and proteolysis results in the release of bone matrix sequestered factors that drive tumor growth. While osteoclasts express numerous proteinases, analysis of human breast to bone metastases unexpectedly revealed that bone forming osteoblasts were consistently positive for the proteinase, MMP-2. Given the role of MMP-2 in extracellular matrix degradation and growth factor/cytokine processing, we tested whether osteoblast derived MMP-2 contributed to the vicious cycle of tumor progression in the bone microenvironment.<h4>Methodology/principal findings</h4>To test our hypothesis, we utilized murine models of the osteolytic tumor-bone microenvironment in immunocompetent wild type and MMP-2 null mice. In longitudinal studies, we found that host MMP-2 significantly contributed to tumor progression in bone by protecting against apoptosis and promoting cancer cell survival (caspase-3; immunohistochemistry). Our data also indicate that host MMP-2 contributes to tumor induced osteolysis (μCT, histomorphometry). Further ex vivo/in vitro experiments with wild type and MMP-2 null osteoclast and osteoblast cultures identified that 1) the absence of MMP-2 did not have a deleterious effect on osteoclast function (cd11B isolation, osteoclast differentiation, transwell migration and dentin resorption assay); and 2) that osteoblast derived MMP-2 promoted tumor survival by regulating the bioavailability of TGFβ, a factor critical for cell-cell communication in the bone (ELISA, immunoblot assay, clonal and soft agar assays).<h4>Conclusion/significance</h4>Collectively, these studies identify a novel "mini-vicious cycle" between the osteoblast and metastatic cancer cells that is key for initial tumor survival in the bone microenvironment. In conclusion, the findings of our study suggest that the targeted inhibition of MMP-2 and/or TGFβ would be beneficial for the treatment of bone metastases. |
| format | Article |
| id | doaj-art-92a678089e744f57baf73db6171a78ed |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj-art-92a678089e744f57baf73db6171a78ed2025-08-20T02:30:56ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0171e2986210.1371/journal.pone.0029862An osteoblast-derived proteinase controls tumor cell survival via TGF-beta activation in the bone microenvironment.Sophie ThiolloyJames R EdwardsBarbara FingletonDaniel B RifkinLynn M MatrisianConor C Lynch<h4>Background</h4>Breast to bone metastases frequently induce a "vicious cycle" in which osteoclast mediated bone resorption and proteolysis results in the release of bone matrix sequestered factors that drive tumor growth. While osteoclasts express numerous proteinases, analysis of human breast to bone metastases unexpectedly revealed that bone forming osteoblasts were consistently positive for the proteinase, MMP-2. Given the role of MMP-2 in extracellular matrix degradation and growth factor/cytokine processing, we tested whether osteoblast derived MMP-2 contributed to the vicious cycle of tumor progression in the bone microenvironment.<h4>Methodology/principal findings</h4>To test our hypothesis, we utilized murine models of the osteolytic tumor-bone microenvironment in immunocompetent wild type and MMP-2 null mice. In longitudinal studies, we found that host MMP-2 significantly contributed to tumor progression in bone by protecting against apoptosis and promoting cancer cell survival (caspase-3; immunohistochemistry). Our data also indicate that host MMP-2 contributes to tumor induced osteolysis (μCT, histomorphometry). Further ex vivo/in vitro experiments with wild type and MMP-2 null osteoclast and osteoblast cultures identified that 1) the absence of MMP-2 did not have a deleterious effect on osteoclast function (cd11B isolation, osteoclast differentiation, transwell migration and dentin resorption assay); and 2) that osteoblast derived MMP-2 promoted tumor survival by regulating the bioavailability of TGFβ, a factor critical for cell-cell communication in the bone (ELISA, immunoblot assay, clonal and soft agar assays).<h4>Conclusion/significance</h4>Collectively, these studies identify a novel "mini-vicious cycle" between the osteoblast and metastatic cancer cells that is key for initial tumor survival in the bone microenvironment. In conclusion, the findings of our study suggest that the targeted inhibition of MMP-2 and/or TGFβ would be beneficial for the treatment of bone metastases.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0029862&type=printable |
| spellingShingle | Sophie Thiolloy James R Edwards Barbara Fingleton Daniel B Rifkin Lynn M Matrisian Conor C Lynch An osteoblast-derived proteinase controls tumor cell survival via TGF-beta activation in the bone microenvironment. PLoS ONE |
| title | An osteoblast-derived proteinase controls tumor cell survival via TGF-beta activation in the bone microenvironment. |
| title_full | An osteoblast-derived proteinase controls tumor cell survival via TGF-beta activation in the bone microenvironment. |
| title_fullStr | An osteoblast-derived proteinase controls tumor cell survival via TGF-beta activation in the bone microenvironment. |
| title_full_unstemmed | An osteoblast-derived proteinase controls tumor cell survival via TGF-beta activation in the bone microenvironment. |
| title_short | An osteoblast-derived proteinase controls tumor cell survival via TGF-beta activation in the bone microenvironment. |
| title_sort | osteoblast derived proteinase controls tumor cell survival via tgf beta activation in the bone microenvironment |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0029862&type=printable |
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